Combining conventional and stroma-derived tumour markers in pancreatic ductal adenocarcinoma

Abstract

Naredi, P. et al. (2015) Combining conventional and stroma-derived tumour markers in pancreatic ductal adenocarcinoma.Cancer Biomarkers, 15 (1) Abstract. BACKGROUND: A lack of disease-specific symptoms and good tumour markers makes early detection and diagnosis of pancreatic ductal adenocarcinoma (PDAC) challenging. OBJECTIVE: To analyse the tissue expression and circulating levels of four stroma-derived substances (type IV collagen, endostatin/type XVIII collagen, osteopontin and tenascin C) and four conventional tumour markers (CA 19-9, TPS, CEA and Ca 125) in a PDAC cohort. METHODS: Tissue expression of markers in normal pancreas and PDAC tissue was analysed with immunofluorescence. Plasma concentrations of markers were measured before and after surgery. Patients with non-malignant disorders served as controls. RESULTS: The conventional and stromal substances were expressed in the cancer cell compartment and the stroma, respectively. Although most patients had increased levels of many markers before surgery, 2/12 (17%) of patients had normal levels of Ca 19-9 at this stage. High preoperative endostatin/type XVIII collagen, and postoperative type IV collagen was associated with short survival. Neither the pre-nor postoperative levels of TPS, Ca 125 or CA 19-9 were associated to survival. CONCLUSIONS: PDAC is characterized by an abundant stroma. These initial observations indicate that the stroma can be a source of PDAC tumour markers that are found in different compartments of the cancer, thus reflecting different aspects of tumour biology.

Diffusion-weighted and dynamic contrast-enhanced MRI of pancreatic adenocarcinoma xenografts: associations with tumor differentiation and collagen content

“…Moreover, the non-differentiated models were found to have more collagen I and collagen IV and a more densely packed matrix of collagen fibers than the differentiated models. Because both poor differentiation and a high level of collagen fibers are associated with poor prognosis in PDAC [ 11 – 13 ], xenografted BxPC-3, Capan-2, MIAPaCa-2, and Panc-1 tumors should be well-suited preclinical models for providing clinically relevant answers to the questions addressed in the present MRI study.…”

“…Collagen is a major component of the stroma, and collagen I is highly up-regulated in PDAC [ 10 ]. Both collagen I and IV have been shown to have prognostic power, and high levels of these matrix components are associated with poor survival rates [ 11 , 12 ].…”

Novel prognostic markers within the CD44‐stromal ligand network in pancreatic cancer

“…Other stromal CD44 ligands include type IV collagen and osteopontin . Both type IV collagen and osteopontin promote pancreatic cancer cell invasion in vitro and are increased in the circulation in pancreatic cancer patients .…”

Biological diagnostic markers and pancreatic cancer risk factors in patients with chronic pancreatitis (literature review)

“…Совсем недавно была опубликована возможность комбинирования обычных и стромальных маркеров в диа гностике РПЖ. Такая комбинация может повысить чувствительность CA 19-9 [10].…”