Combining an innovative non-invasive sampling method and high-throughput sequencing to characterize fungal communities on a canvas painting

Carvalho, H. Paiva de; Sequeira, Sílvia; Pinho, Diogo; Trovão, João; Costa, Ricardo M. F. da; Egas, Conceição; Macedo, Maria Filomena; Portugal, António

doi:10.1016/j.ibiod.2019.104816

Cited by 22 publications

(18 citation statements)

References 38 publications

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“…Conservators of the ICPAL (Istituto Centrale per la Patologia degli Archivi e del Libro) collected samples from the recto and the verso of the selected drawings. A micro-sampling method for fragile surfaces has been specifically developed based on a protocol tested mainly on paintings and described by de Carvalho et al (2019). The system is based on a micro-aspiration aimed primarily at collecting dust particles, and microbial cells from small areas of paper or parchment surfaces (Supplementary Figure 1).…”

Section: Objects and Samplingmentioning

confidence: 99%

“…The species A. glaucus, A. clavatus, and A. niger were dominant in all those drawings. Aspergillus species are well known for the production of cellulases and their main role in the biodeterioration of paper (Das et al, 1997). The most dominant species found in the drawings, namely A. glaucus, is known to have a wide environmental distribution due to its physiological hardiness under extreme conditions and to its ability to grow on substrates with a low water activity (Hubka et al, 2013).…”

Section: Fungimentioning

confidence: 99%

“…Alternaria, with the only detected species A. alternata was present in L3 and L5. This fungus is a wellknown paper-spoiler (Das et al, 1997;Michaelsen et al, 2006;Mesquita et al, 2009) and also considered as an allergen, imposing a severe health threat on librarians, conservators, and scholars who might be in contact with infected paper-supported materials (Karakasidou et al, 2018). It must be mentioned, however, that the Alternaria is a fungus that needs water available in the materials and that it typically develops after flooding and wetting events in building materials, including wood and paper.…”

Section: Fungimentioning

confidence: 99%

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The Microbiome of Leonardo da Vinci’s Drawings: A Bio-Archive of Their History

Piñar

Sclocchi²,

Pinzari³

et al. 2020

Front. Microbiol.

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Seven emblematic Leonardo da Vinci’s drawings were investigated through third generation sequencing technology (Nanopore). In addition, SEM analyses were carried out to acquire photographic documentation and to infer the nature of the micro-objects removed from the surface of the drawings. The Nanopore generated microbiomes can be used as a “bio-archive” of the drawings, offering a kind of fingerprint for current and future biological comparisons. This information might help to create a biological catalog of the drawings (cataloging), a microbiome-fingerprint for each single analyzed drawing, as a reference dataset for future studies (monitoring) and last but not least a bio-archive of the history of each single object (added value). Results showed a relatively high contamination with human DNA and a surprising dominance of bacteria over fungi. However, it was possible to identify typical bacteria of the human microbiome, which are mere contaminants introduced by handling of the drawings as well as other microorganisms that seem to have been introduced through vectors, such as insects and their droppings, visible through the SEM analyses. All drawings showed very specific bio-archives, but a core microbiome of bacteria and fungi that are repeatedly found in this type of material as true degraders were identified, such as members of the phyla Proteobacteria, Actinobacteria, and Firmicutes among bacteria, and fungi belonging to the classes Sordariomycetes and Eurotiomycetes. In addition, some similarities were observed that could be influenced by their geographical location (Rome or Turin), indicating the influence of this factor and denoting the importance of environmental and storage conditions on the specific microbiomes.

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Section: Objects and Samplingmentioning

confidence: 99%

Section: Fungimentioning

confidence: 99%

Section: Fungimentioning

confidence: 99%

See 1 more Smart Citation

The Microbiome of Leonardo da Vinci’s Drawings: A Bio-Archive of Their History

Piñar

Sclocchi²,

Pinzari³

et al. 2020

Front. Microbiol.

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“…Per brevità, invece di descrivere in dettaglio ogni metodo, si rimanda il lettore a una serie di lavori pubblicati, vuoi per i metodi strumentali non-invasivi [2-9], vuoi per le metodiche minimamente invasive [10][11][12][13][14][15][16][17][18]. A questi affianchiamo qui alcune analisi pubblicate di recente, il cui contenuto si può trovare nei riferimenti bibliografici qui riportati [19][20][21][22][23].…”

Section: Introduzioneunclassified

NUOVE ESCHE PER PESCARE NEL <em>MARE MAGNUM DELL’EREDITÀ CULTURALE</em>

Righetti¹,

Zilberstein²

2021

SCIENZE

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We describe here a novel tool for exploring documents pertaining to the world Cultural Heritage while avoiding their contamination or damage. Known under the acronym EVA, it consists of a plastic foil of Ethylene Vinyl Acetate studded with strong cation and anion resins admixed with C8 and C18 hydrophobic beads. When applied to any surface such foils can harvest any type of surface material, which is then eluted and analysed via standard means, such as GS/MS (typically for metabolites), MS/MS (for peptide and protein analysis), X-ray (for elemental analysis). We briefly review here a number of past data, such as screening of original documents by Bulgakov, Chekov, Casanova, Kepler, while dealing in extenso with very recent data, pertaining to Orwell and Stalin and analysis of the skin of an Egyptian mummy. The technique was also successfully applied to paintings, such as the Donna Nuda at the Hermitage in St. Petersburg, attributed to Leonardo and his school. This novel methodology represents a formidable tool for exploring the past life of famous authors, scientist and literates in that it can detect traces of their pathologies and even drug consumption left by saliva and sweat traces on their original hand-written documents.

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“…These techniques include different processes and tools aimed at obtaining the maximum amount of information with the minimum invasiveness of the objects. As an example, an innovative approach developed not long ago is the technique of micro-aspiration using small nitrocellulose membranes (de Carvalho et al, 2019), which allows to obtain all the biological information accumulated on the surface of objects together with deposited dust, impurities and dirt. The next step is the detailed documentation and cataloguing of each of the samples taken (Pinzari et al, 2010) and their distribution to the scientific team for the further investigation, which may include material and microbiological analyses.…”

mentioning

confidence: 99%

Natural sciences at the service of art and cultural heritage: an interdisciplinary area in development and important challenges

Piñar

Sterflinger

2021

Microbial Biotechnology

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Our cultural heritage is a common asset that tells the story of our shared past, is part of our origin and identity and has wide social relevance. Our works of art and our heritage must be enjoyed, appreciated and preserved for future generations. To this end, a wide and varied group of professionals, including conservators, restorers, curators, bibliographers, historians, archivists, but also scientists, such as biologists, chemists, physicists and bioinformaticians, work side by side to preserve our cultural heritage. Working together in this wide range of disciplines included in the so-called 'heritage sciences' is the only plausible way to contribute to the sustainable preservation of our heritage. The great progress made in recent years in conservation and restoration work, but also in the natural sciences considered within heritage science, has provided powerful tools and strategies for analytical and experimental research into historical and cultural objects that open up new frontiers for their diagnosis, monitoring and protection. Here we highlight some of the advances and challenges faced by the natural sciences at the service of art.

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Combining an innovative non-invasive sampling method and high-throughput sequencing to characterize fungal communities on a canvas painting

Cited by 22 publications

References 38 publications

The Microbiome of Leonardo da Vinci’s Drawings: A Bio-Archive of Their History

The Microbiome of Leonardo da Vinci’s Drawings: A Bio-Archive of Their History

NUOVE ESCHE PER PESCARE NEL <em>MARE MAGNUM DELL’EREDITÀ CULTURALE</em>

Natural sciences at the service of art and cultural heritage: an interdisciplinary area in development and important challenges

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