Combining ability for stem borer resistance and heterotic orientation of maize inbred lines using CIMMYT single cross testers under Chilo partellus infestation

Abstract

The spotted stem borer, Chilo partellus, Swinhoe (Lepidoptera, Crambidae) is one of the most destructive insect pests of maize in tropical environments. However, the combining ability and heterotic orientation of the germplasm for grain yield and borer resistance is limited. The objective of this study was to determine combining ability and heterotic orientation of new maize inbred lines under C. partellus infestation. Sixty six (66) inbred lines were crossed to two single cross testers in a line x tester mating scheme. The 132 testcross hybrids and four checks were evaluated at three locations in Kenya under C. partellus infestation. General and specific combining ability effects were significant for C. partellus stem borer resistance traits and grain yield, suggesting the importance of both additive and non-additive gene effects for these traits. Heterotic classification of lines was done based on both heterosis and specific combining ability data. Based on heterosis for grain yield data at Embu, 15 lines were allocated to group A, 18 to group B and 12 to group AB. At Kakamega, 26 lines were oriented towards group A, 19 to group B and 9 to group AB. At Kiboko, 15 lines were inclined towards group A, 18 to group B and 11 to group AB, whilst the remainder could not be classified. Based on the SCA estimates, at Embu, 10 lines revealed positive SCA effects with both testers and were considered to be AB-oriented while 8, and 1 lines were oriented towards A, and B, respectively. A similar trend was detected at Kakamega and Kiboko. The identified lines and heterotic groups would be used by hybrid maize programs where C. partellus stem borers occur exclusively or in league with other stem borers.