Combined X-ray Microfluorescence and Atomic Force Microscopy Studies of Mg Distribution in Whole Cells

Lagomarsino, S.; Iotti, Stefano; Farruggia, Giovanna; Trapani, Valentina; Cedola, Alessia; Fratini, Michela; Bukreeva, Inna; Mastrototaro, Lucia; Notargiacomo, A.; Vogt, Stefan; Kim, S.; Legnini, D.; Maier, Jeanette A.M.; Wolf, Federica I.; McNulty, Ian; Eyberger, Catherine; Lai, Barry

doi:10.1063/1.3625386

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…[143,144] To this end, SRnCT and XRF can be used, which can reveal the intracellular distribution even of light elements Mg, C, N, O, and Na in whole cells. [145,146] Outside the context of Mg implants, it was thus shown, for example, that a colocalization of Zn and Ca and P can be observed during the mineralization process of mesenchymal stem cells, which strengthens the understanding of the role of Zn as nucleation site. [147] Moreover, a combination of SRnCT and X-ray absorption near edge structure (XANES) showed that after 10 days of cell differentiation Ca depositions are mostly present in the form of crystalline hydroxyapatite.…”

Section: In Vitro Degradation Analysismentioning

confidence: 76%

Utilizing Synchrotron Radiation for the Characterization of Biodegradable Magnesium Alloys—From Alloy Development to the Application as Implant Material

et al. 2021

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Magnesium (Mg)-based alloys are investigated for the use as lightweight structural metals, e.g., in the automotive industry, [1] as biodegradable implant materials [2] and for hydrogen storage. [3] The choice of alloying system and subsequent processing of the material is a crucial factor for the degradation profile of the alloy and its mechanical properties. [4][5][6][7] This review focuses on use of Mg alloys as implant material, with some references to its application as a structural metal.The degradation profile of Mg alloys is of particular importance for their application as implant materials, as a controlled degradation is required to ensure cell viability and implant stability in vivo. [8,9] During the development of Mg alloys for the application as a biodegradable implant, alloying systems are carefully selected and manufactured, with their microstructure being tailored according to specifications by selecting the appropriate processing route. The microstructure and the mechanical properties of the alloy will be evaluated and the alloy is then tested for in vitro degradation in an aqueous environment under physiological conditions (pH %7.4, 37 C, 5% CO 2 , 21% O 2 , 95% rel. humidity) with and without cells to assess its general degradation properties and cell viability. [10] The mechanical properties and degradation profile need to be tailored depending on the application of the implant. Mg alloys for bone support, for example, require mechanical properties close to that of bone. For bone, the average Young's modulus is between 7 and 31 GPa, depending on the type of bone and its hydration state. [11,12] In longitudinal direction, the bone's tensile and compressive ultimate strength have been reported to be between 93 and 135 MPa, and 154 and 205 MPa, respectively. [13,14] Finally, the elongation to failure of the clinically approved MAGNEZIX screw by Syntellix AG (Hanover, Germany) was determined to be 8%. [15] By contrast, Mg alloys designed for the use as stents must possess a minimum ultimate tensile strength of 300 MPa, low yield strength of 200-300 MPa, and higher ductility (min. 15%-18% elongation to failure, preferably 30%) for their successful deployment. [16,17] Finally, in vivo animal experiments are conducted to evaluate the alloy suitability for the translation into the clinic.

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Section: In Vitro Degradation Analysismentioning

confidence: 76%

Utilizing Synchrotron Radiation for the Characterization of Biodegradable Magnesium Alloys—From Alloy Development to the Application as Implant Material

et al. 2021

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“…Correlative scanned-beam imaging techniques (x4.6) such as X-ray phase contrast (Hornberger et al, 2007;de Jonge et al, 2008;Holzner et al, 2010;Kosior et al, 2012) or ptychography (Vine et al, 2012) can be used to determine projected mass, which (when coupled with assumptions of the density of the main consituents of the specimen, or the specimen 'matrix') allow one to go from areal content to projected volume concentration. Alternately, one can add measurements of the specimen's thickness using a method such as atomic force microscopy (Lagomarsino et al, 2011), and thus obtain projected elemental concentration directly. Elemental concentration is of great importance, because concentration gradients drive diffusion-based processes and thus play an important role in the biochemical function of cells.…”

Section: X-ray Fluorescence Tomography Of Biological Specimensmentioning

confidence: 99%

X-ray nanoprobes and diffraction-limited storage rings: opportunities and challenges of fluorescence tomography of biological specimens

2014

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Combined X-ray Microfluorescence and Atomic Force Microscopy Studies of Mg Distribution in Whole Cells

Abstract: Abstract. We present in this paper a novel methodology that combines scanning x-ray fluorescencee microscopy and atomic force microscopy. The combination of these two techniques allows the determination of a concentration map of Mg in whole (not sectioned) cells.

Cited by 2 publications

References 6 publications

Utilizing Synchrotron Radiation for the Characterization of Biodegradable Magnesium Alloys—From Alloy Development to the Application as Implant Material

Utilizing Synchrotron Radiation for the Characterization of Biodegradable Magnesium Alloys—From Alloy Development to the Application as Implant Material

X-ray nanoprobes and diffraction-limited storage rings: opportunities and challenges of fluorescence tomography of biological specimens

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