Combined utilization of lipase-displaying Pichia pastoris whole-cell biocatalysts to improve biodiesel production in co-solvent media

Jin, Zi; Han, Sang‐Ik; Zhang, Li; Zheng, Suiping; Wang, Yong; Lin, Ying

doi:10.1016/j.biortech.2012.12.020

Cited by 100 publications

(69 citation statements)

References 35 publications

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“…For example, cells expressing CALB-Gcw21p were collected and combined with cells displaying Rhizomucor miehei lipase as whole-cell biocatalysts to improve biodiesel production in cosolvent media. The results showed that the reaction time and biodiesel yield of the displayed lipase-mediated transesterification reaction were approximately equal to those of commercial lipases (43).…”

Section: Resultsmentioning

confidence: 87%

Screening for Glycosylphosphatidylinositol-Modified Cell Wall Proteins in Pichia pastoris and Their Recombinant Expression on the Cell Surface

Zhang

Liang

Zhou

et al. 2013

Appl Environ Microbiol

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Glycosylphosphatidylinositol (GPI)-anchored glycoproteins have various intrinsic functions in yeasts and different uses in vitro.In the present study, the genome of Pichia pastoris GS115 was screened for potential GPI-modified cell wall proteins. Fifty putative GPI-anchored proteins were selected on the basis of (i) the presence of a C-terminal GPI attachment signal sequence, (ii) the presence of an N-terminal signal sequence for secretion, and (iii) the absence of transmembrane domains in mature protein. The predicted GPI-anchored proteins were fused to an alpha-factor secretion signal as a substitute for their own N-terminal signal peptides and tagged with the chimeric reporters FLAG tag and mature Candida antarctica lipase B (CALB). The expression of fusion proteins on the cell surface of P. pastoris GS115 was determined by whole-cell flow cytometry and immunoblotting analysis of the cell wall extracts obtained by ␤-1,3-glucanase digestion. CALB displayed on the cell surface of P. pastoris GS115 with the predicted GPI-anchored proteins was examined on the basis of potential hydrolysis of p-nitrophenyl butyrate. Finally, 13 proteins were confirmed to be GPI-modified cell wall proteins in P. pastoris GS115, which can be used to display heterologous proteins on the yeast cell surface.

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Section: Resultsmentioning

confidence: 87%

Screening for Glycosylphosphatidylinositol-Modified Cell Wall Proteins in Pichia pastoris and Their Recombinant Expression on the Cell Surface

Zhang

Liang

Zhou

et al. 2013

Appl Environ Microbiol

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“…The immobilized lipase has been studied extensively for biodiesel production due to its faster reaction rate and much lower preparation cost. Several commercial immobilized lipases, such as Novozym 435 and Lipozyme RM IM, have been also used to catalyze the methanolysis of oils (Jin et al 2013). In order to obtain the maximum yield of biodiesel, the effect of four main parameters, including temperature, methanol to oil molar rate, amount of biomass and water content were investigated.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Lipase-Catalyzed Transesterification of Cotton Seed Oil for Biodiesel Production Using Response Surface Methodology

Dong

2016

Braz. arch. biol. technol.

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The aim of this work was to study the biodiesel production from cotton seed oil by lipase produced by Pichia guilliermondii lipase, which was immobilized onto hydrophobic magnetic particles (HMPs). The optimum reaction conditions were determined for lipase dosage, methanol-to-oil molar ratio, temperature and water content. Using response surface methodology, a quadratic polynomial equation was obtained for fatty acid methyl esters (FAMEs) content by multiple regression analysis. Verification experiments confirmed the validity of the predicted model. The optimal conditions for the enzymatic transesterification were temperature of 38.76 , 31.3% immobilized lipase, 10.4% water content, and a methanol-to-oil molar ratio of 4.715:1. The gas chromatography-mass spectrometry showed that biodiesel was mainly composed of the methyl esters of hexadecanoic, 9,12-octadecadienoic and 9-octadecadienoic acid.

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“…Further improvement of the design of the enzymatic reaction with faster flow and a longer residence time is desirable. Comparison of the effect of DME on the conversion ratio of triolein as a function of the reaction time with that of other co-solvents showed that t-butanol may also be useful for enhancing the reaction rate (Royon et al, 2007, Jin et al, 2013, even though the conditions (feed component ratio) employed in the other reports were not the same as those used herein. DME played the role of co-solvent, similar to t-butanol or octanol, in the transesterification by Novozym 435, and DME may be preferable in view of recovery from the produced methyl oleate because of its volatility.…”

Section: Continuous Reactor (Pfr) Systemmentioning

confidence: 82%

“…Enzymatic processes with certain solvents have been studied, and the use of t-butanol (Royon et al, 2007;Li et al, 2010;Jin et al, 2013) as a co-solvent was found to increase the reaction rate; whereas, an ionic liquid (Ruzich and Bassi, 2010) was found to enhance the enzymatic activity, selectivity, and stability.…”

Section: Introductionmentioning

confidence: 99%

“…The objective of this study is to apply the homogeneous transesterification method using DME to the enzymatic production of FAME without alkaline catalysts, and to compare the batch process with the continuous process. The kinetic parameters obtained from the batch process are applied to the continuous process with a pipe reactor and other similar processes (Royon et al, 2007;Jin et al, 2013). …”

Section: Introductionmentioning

confidence: 99%

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Transesterification of Triolein and Methanol by Novozym 435 with Dimethyl Ether

Maeda

Kuramochi

Arafune

et al. 2017

J. Chem. Eng. Japan / JCEJ

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The present study evaluates the lipase (Novozym 435)-catalyzed transesteri cation of triolein and methanol mixtures to generate fatty acid methyl esters (FAMEs) using dimethyl ether (DME) in a batch reactor and a continuous pipe reactor. The addition of DME facilitated the preparation of homogeneous mixtures of triolein and methanol. The e ect of the Novozym 435 and DME contents in the feed mixtures on the time-course of FAME production was analyzed. With increasing contents of Novozym 435 and DME in the feed mixtures, the FAME concentration increased more rapidly in the batch reactor than in the continuous reactor. The feed ow rate and DME content were varied, and the FAME concentration at the outlet of the pipe reactor was measured with variation of the residence time and DME content. Increasing the DME content in the feed mixtures and the residence time led to a more rapid increase in the FAME concentration for the batch reactor than that in the continuous pipe reactor.

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Combined utilization of lipase-displaying Pichia pastoris whole-cell biocatalysts to improve biodiesel production in co-solvent media

Cited by 100 publications

References 35 publications

Screening for Glycosylphosphatidylinositol-Modified Cell Wall Proteins in Pichia pastoris and Their Recombinant Expression on the Cell Surface

Screening for Glycosylphosphatidylinositol-Modified Cell Wall Proteins in Pichia pastoris and Their Recombinant Expression on the Cell Surface

Optimization of Lipase-Catalyzed Transesterification of Cotton Seed Oil for Biodiesel Production Using Response Surface Methodology

Transesterification of Triolein and Methanol by Novozym 435 with Dimethyl Ether

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