Combined use of spinal cord-mimicking partition type scaffold architecture and neurotrophin-3 for surgical repair of completely transected spinal cord in rats

Wang, Xuesong; Li, Yi; Gao, Yinfeng; Xue, Chen; Yao, Jian; Wei, Lin; Chen, Ying; Liu, Jie; Yang, Yumin; Xiao-dong, Wang

doi:10.1080/09205063.2012.727267

Cited by 17 publications

(12 citation statements)

References 32 publications

(34 reference statements)

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“…This effect has been reported for a number of anchorage-dependent cells, such as dorsal root ganglion neurons, Schwann cells [86], neural stem cells [109] and mesenchymal stem cells [110][111][112]. Examples for impregnating the nerve conduit wall with neurite outgrowth promoting factors and neurotrophins include the use of laminin [113,114], neurotrophin-3 [115] and transforming growth factor-β1 [116].…”

Section: Chitosan As An Artificial Nerve Graft Scaffold Fulfills Requmentioning

confidence: 74%

Speculations on the Use of Marine Polysaccharides as Scaffolds for Artificial Nerve ‘Side-’Grafts

Amr¹,

Ekram²

2017

Biological Activities and Application of Marine Polysaccharides

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Marine polysaccharides (e.g., chitosan, alginate and agarose) meet the requirements of artificial nerve grafting. These include the following: (1) prerequisites of biopolymers used as scaffolds; (2) conditions required by nerve autografts; (3) macroengineering requirements (form, design); (4) microengineering requirements (microgrooves, inclusion filaments); (5) mechanical conditions required by nerve autografts; (6) molecular aspects of peripheral nerve regeneration; space and adherence for: (i) chondroitin sulfate proteoglycans, (ii) neurite outgrowth promoting factors, (iii) neurotrophic factors, (iv) cells; (7) artificial side grafts should be compatible with autologous nerve grafts; (8) spatial distribution of neurotrophic factor gradients; (9) modulation of fibrosis; (10) renewal of luminal fillers. The mechanical stability of chitosan should be increased by adding other polymeric chains and cross-linking. As a result of deacetylation of chitin, chitosan scaffolds have got numerous positively charged free amino groups that provide adherence for growth factors and cells. To modulate fibrosis, heparin cross-linked chitosan microspheres have proved effective for delivering/transplanting cells, heparin and growth factors. To renew luminal fillers, chitosan microspheres may be injected through an indwelling catheter incorporated into the nerve conduit. Agarose and alginate have gained more acceptance as hydrogel lumen fillers. Interacting with positively charged chitosan, negatively charged alginate may form versatile chitosan-calcium-alginate microspheres.

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Section: Chitosan As An Artificial Nerve Graft Scaffold Fulfills Requmentioning

confidence: 74%

Speculations on the Use of Marine Polysaccharides as Scaffolds for Artificial Nerve ‘Side-’Grafts

Amr¹,

Ekram²

2017

Biological Activities and Application of Marine Polysaccharides

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“…Complete transection or removal of spinal cord segment. This operation is often performed on adult rats [18][19][20]. To study the regeneration differences between the newborn and adult animals, 5-dayold rats are used also [21].…”

Section: Models Of Traumatic Injuries Of the Spinal Cord In Vivomentioning

confidence: 99%

In vivo and in vitro models of traumatic injuries of the spinal cord

Rybachuk¹,

Arkhypchuk²,

Lazarenko³

2017

JCOT

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In recent years, there is a growing interest in the mechanisms of regeneration of damaged nerve tissue, including the spinal cord, as its injuries are quite common due to traffic accidents, industrial injuries and military actions. Damage to the spinal cord results in the loss of functional activity of the body below the injury site, which affects person’s ability to self-service and significantly reduces its efficiency. The effects of spinal injuries annually cause significant social and economic losses worldwide, including Ukraine. The development of new treatments for pathologies of the central nervous system requires mandatory pre-testing of their effectiveness in experiments in vitro and in vivo. Therefore, searching and creation of optimal animal model of spinal cord injury is in order to it meets most complete picture of the damage characteristic of real conditions in humans. This is an important task of modern neurophysiology. Such models can be used, primarily, for a more detailed clarification of the pathogenesis of all levels of nerve tissue damage and research of its own recovery potential by endogenous reparation mechanisms. In addition, experimental models allow to estimate the safety and predict the effectiveness of various therapeutic approaches to spinal cord injury.

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“…Neurotrophic factors may be applied by the following three methods: local injection, cerebrospinal fluid injection and gene modification (80). Of these methods, the most important is gene modification.…”

Section: Growth Factormentioning

confidence: 99%

Tissue engineering is a promising method for the repair of spinal cord injuries (Review)

Zhou

et al. 2013

Experimental and Therapeutic Medicine

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Spinal cord injury (SCI) may lead to a devastating and permanent loss of neurological function, which may place a great economic burden on the family of the patient and society. Methods for reducing the death of neuronal cells, inhibiting immune and inflammatory reactions, and promoting the growth of axons in order to build up synapses with the target cells are the focus of current research. Target cells are located in the damaged spinal cord which create a connect with the scaffold. As tissue engineering technology is developed for use in a variety of different areas, particularly the biomedical field, a clear understanding of the mechanisms of tissue engineering is important. This review establishes how this technology may be used in basic experiments with regard to SCI and considers its potential future clinical use.

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Combined use of spinal cord-mimicking partition type scaffold architecture and neurotrophin-3 for surgical repair of completely transected spinal cord in rats

Cited by 17 publications

References 32 publications

Speculations on the Use of Marine Polysaccharides as Scaffolds for Artificial Nerve ‘Side-’Grafts

Speculations on the Use of Marine Polysaccharides as Scaffolds for Artificial Nerve ‘Side-’Grafts

In vivo and in vitro models of traumatic injuries of the spinal cord

Tissue engineering is a promising method for the repair of spinal cord injuries (Review)

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