Combined use of Enterobacter cloacae MB20 and the microelements of copper and manganese to control damping-off of tomato

Abstract

Biocontrol activity of Enterobacter cloacae alone and in combination with copper and mangan on the suppression of tomato damping off was assessed. Rhizoctonia solani damping-off is difficult to control, since its dorman organ sclerotia survive for long periods under various environmental condition. The availability of micronutrients copper and mangan may play important roles in controlling of plant diseases. Results showed that the application of E. cloacae alone decreased damping-off of tomato significantly (P<0.05) by 85%. Combination application of E. clocae with 10μg ml−1 of CuSO4 showed the highest suppression of the disease, followed by the treatment of E. cloacae with 5μg ml−1, as high as 95% and 90% respectively, although the differences was not significant compared to other treatments. Degree of the disease suppression did not differ between application of E. cloacae alone and in combination with both concentration of MnSO4 (μg ml−1 and10μg ml−1), the disease decreased about 85%. Inoculation of R. solani alone into soil resulted significantly highest (P<0.05) of fungal population, it was about 33.7 x 103 CFU g−1 soil, and inoculation of R. solani and E. cloacae reduced significantly fungal population to about 19.0 x103 CFU g−1 soil. E. cloacae produced chitinase and protease, its chitinolytic index was about 0.84, and proteolytic index was 0.61.

Approving the biocontrol method of potato wilt caused by Ralstonia solanacearum (Smith) using Enterobacter cloacae PS14 and Trichoderma asperellum T34

“…For example, Götz et al (2006) used Enterobacter cowanii as biocontrol agents against bacterial wilt of tomato. E. cloacae was used successfully for controlling many plant diseases such as Fusarium wilt of spinach (Tsuda et al 2001), bacterial blight of rice (Yang et al 2000), dry rot of potato (Al-Mughrabi 2010), and damping-off of tomato (Yuliar et al 2019).…”

Variation of the rate of pesticides decomposition used together in the process of agricultural production

“…In all variants using tank mixtures, variation in the rate of compound of individual active substances was recorded in comparison with their separate use. According to some studies, the combined use of copper-containing preparations with other chemical preparations to varying degrees affects the decomposition of active pesticides in the green mass of plants (Yuliar et al, 2019;Zakharychev, 2020). It is also known that copper-based preparations can also affect the permeability of plant cell walls (Yurkevich et al, 2011;Zakharychev, 2020).…”

Rhizobacterial Control of Root Diseases and Stimulation of Plant Growth

“…Chitinases are especially able to antagonize fungi whose cell walls contain chitin, which is essential for structural stability providing anchor points for other components at the cell surface. Bacteria secreting chitinases have been shown control fungal pathogens including Botrytis cinerea and R. solani, reducing the incidence of these related crop diseases in field trials by 76% and 84% respectively [88,247]. In addition to their antagonistic activity, growth-promoting bacteria with chitinolytic activity have also been associated with plant growth; for example, bacteria of the genera Pseudomonas and Bacillus with high chitinolytic activity increased germination in pigeon pea (Cajanus cajan) and in another example, a root inoculation with a chitinolytic strain of Streptomyces was associated with increased biomass in chickpea [245,248].…”