Combined Use of Autologous Stromal Vascular Fraction Cells and Platelet-Rich Plasma for Chronic Ulceration of the Diabetic Lower Limb Improves Wound Healing

Abstract

The discovery of stromal vascular fraction cells and platelet-rich plasma in promoting tissue regeneration has prompted a new idea for the treatment of chronic diabetic ulcer of the lower limb. The study aim was to evaluate the clinical efficacy of a new method that applied stromal vascular fraction cells and platelet-rich plasma together in the treatment of recalcitrant chronic diabetic ulcer. We conducted a single-center, prospective, open, noncontrolled study. Four patients (5 ulcers in total) who had received standard treatment for diabetic ulcer for at least 3 months that failed to heal was enrolled. All patients were treated with surgical debridement, cell suspension (stromal vascular fraction cells suspended by platelet-rich plasma) injection into the wound, and platelet-rich plasma gel coverage. Wounds were measured every week after treatment using a 2-dimensional digital camera and a 3-dimensional wound measurement device. All patients were followed-up for 4 months after the treatment. Four of the 5 ulcers healed completely within a mean of 71.75 ± 29.57 days. The average proportion of granulation tissue achieved 100% within 4 weeks for all cases. The wound size decreased to less than half of the original size for all cases 4 weeks after the treatment. Findings revealed that the new treatment is efficient to achieve wound healing in patients with recalcitrant chronic diabetic ulcer of lower limb.

Xenobiotic-Free Medium Guarantees Expansion of Adipose Tissue-Derived Canine Mesenchymal Stem Cells Both in 3D Fibrin-Based Matrices and in 2D Plastic Surface Cultures

“…Interestingly, both PRP and SVF application have been proposed in recent years to improve wound healing [43][44][45]. Yin et al proposed that the synergistic effects of PRP and SVF promote a safe and effective wound healing in chronic ulcers in humans [46]. Our data suggest that the combination of the two regenerative approaches can be accomplished in the dog.…”

Evaluating the quality of studies reporting on clinical applications of stromal vascular fraction: A systematic review and proposed reporting guidelines (CLINIC-STRA-SVF)

“…Of these, 77 articles were selected after reading the titles and abstracts and removing duplicates. Then, 15 studies were removed because they met the exclusion criteria, leaving 62 studies [ [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] , [41] , [42] , [43] , [44] , [45] , [46] , [47] , [48] , [49] , [50] , [51] , [52] , [53] , [54] , [55] , [56] , [57] , [58] , [59] , [60] , [61] , [62] , [63] , [64] , [65] , [66] , [67] , [68] , [69] , [70] , [71] , [72] , [73] , [74] , [75] , [76] , [77] , [78] , [79] , [80] , [81] , [82] , [83] , [84] , [85] , [86] , [87] , [88] , [89] ]. ( Fig.…”

Enhancement and Repair of Degenerative Intervertebral Disc in Rats Using Platelet-Rich Plasma/Ferulic Acid Hydrogel

“…Many studies have shown that PRP promotes the proliferation of NP cells, facilitates the anabolic actions and migration of different cell types, and provides a favorable microenvironment for the regeneration and repair of various soft tissues. 21 , 22 The study by Chen et al 23 cultured human NP cells in PRP and found that PRP could enhance cell proliferation and differentiation through transforming growth factor beta-1, demonstrating for the first time that PRP is a therapeutic option for preventing disc degeneration. In a study of 47 patients with lumbar disc degeneration, Tuakli-wosornu et al 12 found significant improvements in Functional Rating Index, Numerical Rating Scale of Pain, and North American Spine Society satisfaction scores in participants who received intradiscal PRP compared with those who received contrast alone.…”