Combined treatment with minocycline and prednisone attenuates experimental autoimmune encephalomyelitis in C57 BL/6 mice

Chen, Xiaohong; Zou, Yan; Liu, Mei; Wang, Tieqiao; Zheng, Xueping; Li, Meng; Lin, Mingxing; Liu, Peiqing; Tao, Liang

doi:10.1016/j.jneuroim.2009.02.016

Cited by 29 publications

(14 citation statements)

References 35 publications

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“…The present data confirm the previously reported susceptibility of MOG‐induced EAE in C57BL/6 mice to prophylactic and early therapeutic treatment with GCs [30–32]. However, the lack of effect of CY in this model that may mirror more closely PP or SP forms of MS according to the criteria and definitions as proposed by Lublin et al.…”

Section: Discussionsupporting

confidence: 91%

Specific and Strain-Independent Effects of Dexamethasone in the Prevention and Treatment of Experimental Autoimmune Encephalomyelitis in Rodents

Donia

Mangano

Quattrocchi

et al. 2010

Scandinavian Journal of Immunology

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Experimental autoimmune encephalomyelitis in rodents (EAE) is a generally accepted in vivo model for immunopathogenic mechanisms underlying multiple sclerosis (MS). There are, however, different forms of rodent EAE, and therapeutic regimens may affect these forms differently. We have therefore tested the effects of dexamethasone (Dex) and found that both prophylactic and early therapeutic regimens were effective in suppressing the development of monophasic EAE in myelin basic protein‐immunized Lewis rats, the relapsing–remitting forms of EAE induced in SJL mice by proteolipid protein and in DA rats by syngeneic spinal cord homogenate, and the progressive forms induced in C57BL/6 and DBA/1 mice by immunization with myelin oligodendrocyte glycoprotein. In addition, prophylactically administered Dex suppressed histological and immunological features of EAE such as spinal cord infiltration of inflammatory cells and the increased frequency of autoantigen‐specific interferon‐gamma‐secreting lymph node mononuclear cells. The present data reproduced in rodent EAE models some of the beneficial effects observed with glucocorticoids in MS. This strengthens the validity of these five models as in vivo predictors of drug efficacy in at least some variants of human MS. Better understanding of the clinical and immunopharmacologic features of these models might prove useful when testing new drug candidates for MS treatment.

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Section: Discussionsupporting

confidence: 91%

Specific and Strain-Independent Effects of Dexamethasone in the Prevention and Treatment of Experimental Autoimmune Encephalomyelitis in Rodents

Donia

Mangano

Quattrocchi

et al. 2010

Scandinavian Journal of Immunology

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“…EAE was induced in six to eight-week-old female C57 BL/6 mice by immunization with MOG 35-55 as previously described [16]. Berberine was intragastric administration via per os and the therapy was started at the disease onset and medication was administered daily until mice were sacrificed at day 35 post immunization (p.i.).…”

Section: Resultsmentioning

confidence: 99%

“…EAE was induced in six to eight-week-old female C57 BL/6 mice by the procedure which had been described previously [16]. Briefly, mice were immunized subcutaneously in the flanks with 200 mg of MOG35-55 peptide per animal emulsified in complete Freund's adjuvant (CFA) containing 500 µg of Mycobacterium tuberculosis H37RA.…”

Section: Methodsmentioning

confidence: 99%

Berberine Attenuates Experimental Autoimmune Encephalomyelitis in C57 BL/6 Mice

Jiang

et al. 2010

PLoS ONE

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BackgroundBerberine, an isoquinoline derivative alkaloid, has a wide range of pharmacological properties and is considered to have anti-inflammatory and neuroprotective effects. However, there are no reports about the effects and mechanisms of berberine in experimental autoimmune encephalomyelitis (EAE), an established model of multiple sclerosis (MS).Methodology/Principal FindingsFemale C57 BL/6 mice immunized with myelin oligodendrocyte glycoprotein 35–55 amino acid peptide were treated with berberine at the day of disease onset and medication was administered daily until mice were sacrificed. Blood–brain barrier (BBB) permeability and the alteration of matrix metalloproteinase-2 (MMP-2, 72 kDa) and matrix metalloproteinase-9 (MMP-9, 92 kDa) in the brain and cerebrospinal fluid (CSF) of EAE mice were detected by quantitative measurement for Evan's blue (EB) content, Western blot and gelatin zymography respectively. The results showed that berberine attenuated clinical and pathological parameters of EAE, reduced the permeability of BBB, inhibited the activity and expression of MMP-9 but not MMP-2 in the CSF and brain of EAE mice.Conclusions/SignificanceThese findings suggest that berberine is effective to attenuate the clinical severity of EAE in C57 BL/6 mice by reducing the permeability of BBB, decreasing the expression and activity of MMP-9, and decreasing the inflammatory infiltration. We think that berberine might be a potential therapeutic agent for MS.

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“…The procedure used for the induction of EAE has been described previously (Chen et al, 2009). Briefly, mice received a subcutaneous injection in the flanks with 200 μg MOG 35-55 peptide emulsified in CFA containing 500 μg mycobacterium tuberculosis H37RA.…”

Section: Induction and Assessment Of Eaementioning

confidence: 99%

Total glucosides of peony attenuates experimental autoimmune encephalomyelitis in C57BL/6 mice

Huang

Zhu

et al. 2015

Journal of Neuroimmunology

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Combined treatment with minocycline and prednisone attenuates experimental autoimmune encephalomyelitis in C57 BL/6 mice

Cited by 29 publications

References 35 publications

Specific and Strain-Independent Effects of Dexamethasone in the Prevention and Treatment of Experimental Autoimmune Encephalomyelitis in Rodents

Specific and Strain-Independent Effects of Dexamethasone in the Prevention and Treatment of Experimental Autoimmune Encephalomyelitis in Rodents

Berberine Attenuates Experimental Autoimmune Encephalomyelitis in C57 BL/6 Mice

Total glucosides of peony attenuates experimental autoimmune encephalomyelitis in C57BL/6 mice

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