Combined treatment of human MCF-7 breast carcinoma with antibody, cationic lipid and hyaluronic acid using ex vivo assays

Bartusik, Dorota; Tomanek, Bogusław; Lattová, Erika; Perreault, Hélène; Fallone, B. G.

doi:10.1016/j.jpba.2009.07.032

Cited by 18 publications

(29 citation statements)

References 32 publications

(22 reference statements)

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“…This undesirable non‐human NeuGc has been discussed as being potentially immunogenic and unacceptable as a therapeutic agent (Jefferis, ). The evaluation of cell viability demonstrated that lipoplex targeted samples generally resulted in a more efficient delivery of the antibody into cancer cells and produced a higher growth‐inhibitory effect than the monoclonal antibody administrated alone (Bartusik et al, ). The results in a combination with MS evaluation of profiles supported the hypothesis that some N ‐glycans detected in cells exposed to antibody without Lipoplex may be associated with the development of cancer cells' resistance against the antibody.…”

Section: Application Of Hydrazine Derivatives In Proteomic Studiesmentioning

confidence: 99%

The usefulness of hydrazine derivatives for mass spectrometric analysis of carbohydrates

Lattová

Perreault

2013

Mass Spectrometry Reviews

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Over the last years, extensive studies have evaluated glycans from different biological samples and validated the importance of glycosylation as one of the most important post-translational modifications of proteins. Although a number of new methods for carbohydrate analysis have been published and there has been significant progress in their identification, the development of new approaches to study these biomolecules and understand their role in living systems are still vivid challenges that intrigue glycobiologists. In the last decade, the success in analyses of oligosaccharides has been driven mainly by the development of innovative, highly sensitive mass spectrometry techniques. For enhanced mass spectrometry detection, carbohydrate molecules are often derivatized. Besides, the type of labeling can influence the fragmentation pattern and make the structural analysis less complicated. In this regard, in 2003 we introduced the low scale, simple non-reductive tagging of glycans employing phenylhydrazine (PHN) as the derivatizing reagent. PHN-labeled glycans showed increased detection and as reported previously they can be analyzed by HPLC, ESI, or MALDI immediately after derivatization. Under tandem mass spectrometry conditions, PHN-derivatives produced useful data for the structural elucidation of oligosaccharides. This approach of analysis has helped to reveal new isomeric structures for glycans of known/unknown composition and has been successfully applied for the profiling of N-glycans obtained from serum samples and cancer cells. The efficacy of this labeling has also been evaluated for different substituted hydrazine reagents. This review summarizes all types of reducing-end labeling based on hydrazone-linkage that have been used for mass spectrometric analyses of oligosaccharides. This review is also aimed at correcting some past misconceptions or interpretations reported in the literature.

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Section: Application Of Hydrazine Derivatives In Proteomic Studiesmentioning

confidence: 99%

The usefulness of hydrazine derivatives for mass spectrometric analysis of carbohydrates

Lattová

Perreault

2013

Mass Spectrometry Reviews

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“…Four different breast carcinoma cell lines, possessing different invasion abilities, were applied in our experiment: two cell lines with low invasion abilities (MCF7 and T-47D) and two with higher invasion abilities (MDA-MB-231 and MCF7-Adr) [13,14].…”

Section: Cell Culture and Transfectionmentioning

confidence: 99%

RACK1 promotes breast carcinoma proliferation and invasion/metastasis in vitro and in vivo

Cao

et al. 2009

Breast Cancer Res Treat

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A yeast two-hybrid system was utilized to identify novel PI3K p110alpha-interacting proteins, of which receptor of activated protein kinase C1 (RACK1) was chosen for successive detailed analyses. Our aim was to investigate the function(s) of RACK1 and its involvement in mechanisms of breast carcinoma proliferation and invasion/metastasis. Experiments in breast carcinoma cell lines stably transfected with RACK1, as well as nude mouse models, showed that RACK1 promotes breast carcinoma proliferation and invasion/metastasis in vitro and in vivo. Conversely, knockdown of RACK1 by siRNA in vitro inhibited proliferation, migration, and invasion. In cell lines stably transfected with RACK1, p-AKT, cyclin D1, cyclin D3, and CD147 expression, as well as MMP2 activity, were elevated. RACK1-induced migration could be inhibited by the addition of Rho-kinase inhibitor. In 160 breast carcinoma cases, survival analyses established that RACK1 is an independent prognostic factor for poor outcome (P < 0.001). In conclusion, RACK1 is an independent prognosis-related factor and promotes breast carcinoma proliferation and invasion/metastasis in vitro and in vivo.

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“…These facts and the consistency of the data presented on two different cancer cell models suggest that all of these new glycosylated peptides described here are results of unknown transformation in the IgG1 molecule itself during treatment. As we have previously shown, transfection of cancer cells with Herceptin/Lipoplex decreased the viability of these cells to a significantly higher degree than treatment with Herceptin alone (29). Lipofectamine is known to alter the plasma membrane but it remains unclear how it enhances the effects of Herceptin.…”

Section: Discussionmentioning

confidence: 89%

“…Both cell samples were cultured in a Hollow Fiber Bioreactor device (HFB, FiberCell Systems, Frederick, MD) as previously described (29,30). Briefly, cells were grown at 37°C under 5% of carbon dioxide and air.…”

Section: Methodsmentioning

confidence: 99%

Alterations in Glycopeptides Associated with Herceptin Treatment of Human Breast Carcinoma MCF-7 and T-Lymphoblastoid Cells

Lattová

Bartusik²,

Spicer

et al. 2011

Molecular & Cellular Proteomics

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The therapeutic humanized monoclonal antibody IgG1 known as Herceptin ® has shown remarkable antitumor effects. Although this type of therapy has increased the cancer-free survival of patients, not all tumors respond to this treatment and cancers often develop resistance to the antibody. Despite the fact that Herceptin function has been extensively studied, the precise mechanism underlying its antitumor activity still remains incompletely defined. We previously demonstrated on human breast MCF-7 carcinoma and T-lymphoblastoid CEM cells that monoclonal antibody in combination with Lipoplex consisting of Lipofectamine mixed with plasmid DNA showed a more profound effect on cancer cell viability than antibody alone. The analyses of N-glycans isolated from cancer cells showed dramatic differences in profiles when cells were exposed to Herceptin. Moreover, the investigation of glycosylated peptides from the same cancer cell models after treatment revealed further alterations in the post-translational modifications. Tandem mass spectra obtained from the samples treated confirmed the presence of a series of glycopeptides bearing characteristic oligosaccharides as described in IgG1. However some of them differed by mass differences that corresponded to peptide backbones not described previously and more of them were detected from Herceptin treated samples than from cells transfected with Heceptin/Lipoplex. The results indicate that the presence of Lipoplex prevents antibody transformation and elongates its proper function. The better understanding of the multipart changes described in the glycoconjugates could provide new insights into the mechanism by which antibody induces regression in

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Combined treatment of human MCF-7 breast carcinoma with antibody, cationic lipid and hyaluronic acid using ex vivo assays

Cited by 18 publications

References 32 publications

The usefulness of hydrazine derivatives for mass spectrometric analysis of carbohydrates

The usefulness of hydrazine derivatives for mass spectrometric analysis of carbohydrates

RACK1 promotes breast carcinoma proliferation and invasion/metastasis in vitro and in vivo

Alterations in Glycopeptides Associated with Herceptin Treatment of Human Breast Carcinoma MCF-7 and T-Lymphoblastoid Cells

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