Combined targeted/untargeted analytical and chemometric approaches in the characterization of Daphnia magna metabolomic changes under bisphenol A exposure

Garreta-Lara, Elba; Gómez‐Canela, Cristian; Campos, Bruno; Barata, Carlos; Tauler, Romà; Lacorte, Sı́lvia

doi:10.1016/j.microc.2021.106150

Cited by 8 publications

(7 citation statements)

References 45 publications

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“…Nagato et al [39] employed different BPA concentrations and reported an increase in the concentration of amino acids for exposure groups with higher concentrations of BPA and attributed this observation to a reduction in protein synthesis to counterbalance the consequences of toxic stress. In a 24-h D. magna exposure to BPA, Garreta-Lara et al [41] did not observe a unique tendency of increase or decrease for the concentration of amino acids. Garreta-Lara et al [41] also reported a downregulation in the biosynthesis of fatty acids and suggested a major disruption to sugar and lipid metabolism was responsible for the metabolic responses, rather than a response to toxic stress.…”

Section: Discussionmentioning

confidence: 90%

“…In a 24-h D. magna exposure to BPA, Garreta-Lara et al [41] did not observe a unique tendency of increase or decrease for the concentration of amino acids. Garreta-Lara et al [41] also reported a downregulation in the biosynthesis of fatty acids and suggested a major disruption to sugar and lipid metabolism was responsible for the metabolic responses, rather than a response to toxic stress. This disturbance may result in amino acids being used as a carbon source for energic requirements, which agrees with our observations for all three bisphenols.…”

Section: Discussionmentioning

confidence: 90%

“…Metabolomic-based investigations identify responses that are directly linked to the functional state of an organism, which can be useful when studying pollutant toxicity at sub-lethal or environmentally relevant concentrations [36,38]. Although studies trying to understand how BPA alters the metabolic profile of D. magna [39][40][41] have reported biochemical perturbations at sub-lethal exposure concentrations, there is no information about the BPA analogues, BPF and BPS. D. magna is a small crustacean that occupies an intermediate level in many aquatic food chains, has a short lifespan and is highly sensitive to many pollutants [36,42].…”

Section: Introductionmentioning

confidence: 99%

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Metabolomics Reveals That Bisphenol Pollutants Impair Protein Synthesis-Related Pathways in Daphnia magna

et al. 2021

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Bisphenols are used in the production of polycarbonate plastics and epoxy resins. Bisphenol A (BPA) has been widely studied and is believed to act as an endocrine disruptor. Bisphenol F (BPF) and bisphenol S (BPS) have increasingly been employed as replacements for BPA, although previous studies suggested that they yield similar physiological responses to several organisms. Daphnia magna is a common model organism for ecotoxicology and was exposed to sub-lethal concentrations of BPA, BPF, and BPS to investigate disruption to metabolic profiles. Targeted metabolite analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to measure polar metabolites extracted from D. magna, which are linked to a range of biochemical pathways. Multivariate analyses and individual metabolite changes showed similar non-monotonic concentration responses for all three bisphenols (BPA, BPF, and BPS). Pathway analyses indicated the perturbation of similar and distinct pathways, mostly associated with protein synthesis, amino acid metabolism, and energy metabolism. Overall, we observed responses that can be linked to a chemical class (bisphenols) as well as distinct responses that can be related to each individual bisphenol type (A, F, and S). These findings further demonstrate the need for using metabolomic analyses in exposure assessment, especially for chemicals within the same class which may disrupt the biochemistry uniquely at the molecular-level.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

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Metabolomics Reveals That Bisphenol Pollutants Impair Protein Synthesis-Related Pathways in Daphnia magna

et al. 2021

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“…Mass spectrometry (MS) has received attentions for its advantages of simple operation, fast speed, high resolution and high sensitivity, and has become a necessary method for the identification of microorganisms. [16][17][18] With the rapid development of MS technology in microbial identification, MS has been gradually applied to the studies on bacterial drug resistance in microbiology laboratory. [19,20] Lau et al [21] used a matrix-assisted laser desorption ionization timeof-flight mass spectrometry (MALDI-TOF MS) to realize the identification and direct tracking of plasmids carrying carbapenemase genes.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rapid Identification of Polypeptide from Carbapenem‐Resistant and SusceptibleEscherichia colivia Orbitrap‐MS and Pattern Recognition Analyses

Sun

Wang

et al. 2022

Chemistry & Biodiversity

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A rapid and accurate analytical method was established to identify CREC and CSEC. Orbitrap-MS was used to detect the polypeptide of CREC and CSEC strains, and MS data were analyzed by pattern recognition analyses such as hierarchical cluster analysis (HCA), principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and orthogonal partial least squares discriminant analysis (OPLS-DA). HCA based on the farthest distance method could well distinguish the two types of E. coli, and the cophenetic correlation coefficient of the farthest distance method was 0.901. Comparing the results of PCA, PLS-DA, and OPLS-DA, OPLS-DA exhibited the highest accuracy in predicting the CREC and CSEC strains. A total of 26 compounds were identified, and six of the compounds were the highly significant difference between the two types of strains. MS combined with pattern recognition can achieve a more comprehensive and efficient statistical analysis of complex biological samples.

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Metabolomics and lipidomics signature in celiac disease: a narrative review

Rostami-Nejad,

Asri,

Bakhtiari

et al. 2024

Clin Exp Med

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Celiac disease (CD) is a chronic immune-mediated inflammatory disease of the small intestine caused by aberrant immune responses to consumed gluten proteins. CD is diagnosed by a combination of the patients reported symptoms, serologic and endoscopic biopsy evaluation of the small intestine; and adherence to a strict gluten-free diet (GFD) is considered the only available therapeutic approach for this disorder. Novel approaches need to be considered for finding new biomarkers to help this disorder diagnosis and finding a new alternative therapeutic method for this group of patients. Metabolomics and lipidomics are powerful tools to provide highly accurate and sensitive biomarkers. Previous studies indicated a metabolic fingerprint for CD deriving from alterations in gut microflora or intestinal permeability, malabsorption, and energy metabolism. Moreover, since CD is characterized by increased intestinal permeability and due to the importance of membrane lipid components in controlling barrier integrity, conducting lipidomics studies in this disorder is of great importance. In the current study, we tried to provide a critical overview of metabolomic and lipidomic changes in CD.

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Combined targeted/untargeted analytical and chemometric approaches in the characterization of Daphnia magna metabolomic changes under bisphenol A exposure

Cited by 8 publications

References 45 publications

Metabolomics Reveals That Bisphenol Pollutants Impair Protein Synthesis-Related Pathways in Daphnia magna

Metabolomics Reveals That Bisphenol Pollutants Impair Protein Synthesis-Related Pathways in Daphnia magna

Rapid Identification of Polypeptide from Carbapenem‐Resistant and SusceptibleEscherichia colivia Orbitrap‐MS and Pattern Recognition Analyses

Metabolomics and lipidomics signature in celiac disease: a narrative review

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