Combined surface acoustic wave and surface plasmon resonance measurement of collagen and fibrinogen layer physical properties

Abstract

We use an instrument combining optical (surface plasmon resonance) and acoustic (Love mode surface acoustic wave device) real-time measurements on a same surface for the identification of water content in collagen and fibrinogen protein layers. After calibration of the surface acoustic wave device sensitivity by copper electrodeposition and surfactant adsorption, the bound mass and its physical properties -density and optical index -are extracted from the complementary measurement techniques and lead to thickness and water ratio values compatible with the observed signal shifts. Such results are especially usefully for protein layers with a high water content as shown here for collagen on an hydrophobic surface. We obtain the following results: collagen layers include 70±20% water and are 16±3 to 19±3 nm thick for bulk concentrations ranging from 30 to 300 µg/ml. Fibrinogen layers include 50±10% water for layer thicknesses in the 6±1.5 to 13±2 nm range when the bulk concentration is in the 46 to 460 µg/ml range.

Multifunctional and Sensitivity Enhancement of Hybrid Acoustoplasmonic Sensors Fabricated on 36XY-LiTaO3 with Gold Nanoparticles for the Detection of Permittivity, Conductivity, and the Refractive Index

“…In recent years, several studies have focused on designing multifunctional sensors for integrated and diverse applications − to support Societies 5.0. These multifunctional sensors have several advantages, such as low cost, low energy consumption, light weight, and containing massive information. , Several interesting materials and phenomena, such as ionic materials, , nanocarbon structures, graphene oxide, − piezoelectric sensors based on zinc oxide materials, − film bulk acoustic wave resonators (FBARs), , quartz crystal microbalances (QCMs), , Rayleigh surface acoustic waves (R-SAWs), − and shear horizontal surface acoustic waves (SH-SAWs), − are exploited to develop multifunctional sensors. In general, to produce multifunctional sensing performance, two or more devices should be combined.…”

“…QCMs are another type of piezoelectric material sensor. Friedt et al . proposed and combined a QCM sensor with a surface plasmon resonance (SPR) device for collagen/fibrinogen sensing.…”

“…QCMs are another type of piezoelectric material sensor. Friedt et al 21 proposed and combined a QCM sensor with a surface plasmon resonance (SPR) device for collagen/fibrinogen sensing. However, the SPR sensor has drawbacks, such as its need for a prism; therefore, the measured system is complex.…”

Hybrid Sensor Device for Simultaneous Surface Plasmon Resonance and Surface Acoustic Wave Measurements

“…From a technical point of view, combining these two technologies is feasible by exploiting the geometrical structure of the SAW device, which leaves the rear of the piezoelectric element free for a light beam to propagate and to produce surface plasmons on a gold coated sensing area (Figure 1). Based on the above, a set-up for simultaneous SPR/LW-SAW measurements was developed and used to study the thickness and protein-to-water content ratio of several protein layers [16,17]. To incorporate energy losses, the same group employed the quartz crystal microbalance with dissipation monitoring (QCM-D) and succeeded in obtaining information on the conformation of human immunoglobulin protein G (hIgG) [18].…”

Analysis on Characteristics of ZnO Surface Acoustic Wave with and without Micro-Structures

“…The research in [11] aimed to provide the reader with a historical basis, routes for more detailed study, and an impression of future directions in the field. A two-step measurement, using copper eletro-deposition for instrument calibration followed by a measurement on the actual biological layer under investigation in a combined SAW/SPR instrument, led to an estimate of water content and layer thickness [12]. The influence of surface structure in terms of SAW biosensor signals has been discussed.…”