Combined Strategy for a Reliable Evaluation of Spinal Cord Injury Using an in vivo Model

Gómez, Rosa Margarita; Ghotme, Kemel A.; Nino, Jackeline J; Quiroz‐Padilla, María Fernanda; Vargas, Daniela Suárez; Dominguez, Andy R; Barreto, George E.; Sanchez, Magdy Y

doi:10.2174/1871524915666150819104114

Cited by 3 publications

(3 citation statements)

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“…Local reparative activation was evident in the injured SC tissue, leading to functional recovery as evaluated using the BBB scale (Botero, ; Gomez, ). This combination functioned in a similar way in chronic and acute SCI (Botero, ) when evaluated using a neurological assessment (Gomez et al, ). Transplanted rats showed motor and sensory recovery in both the acute and chronic phase.…”

Section: Oecs In Spinal Cord Injury (Sci) Repairmentioning

confidence: 94%

“…Local reparative activation was evident in the injured SC tissue, leading to functional recovery as evaluated using the BBB scale (Botero, 2015;Gomez, 2009). This combination functioned in a similar way in chronic and acute SCI (Botero, 2015) when evaluated using a neurological assessment (Gomez et al, 2015). Transplanted rats showed motor and sensory (Huang et al, 2009), and in patients with amyotrophic lateral sclerosis or Friedreich's Ataxia, it is difficult to ascertain the contribution of the OEC component in these bulb-derived cell mixtures.…”

Section: Om-mentioning

confidence: 97%

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Cell therapy for spinal cord injury with olfactory ensheathing glia cells (OECs)

Gómez

Sanchez

Portela-Lomba

et al. 2018

Glia

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The prospects of achieving regeneration in the central nervous system (CNS) have changed, as most recent findings indicate that several species, including humans, can produce neurons in adulthood. Studies targeting this property may be considered as potential therapeutic strategies to respond to injury or the effects of demyelinating diseases in the CNS. While CNS trauma may interrupt the axonal tracts that connect neurons with their targets, some neurons remain alive, as seen in optic nerve and spinal cord (SC) injuries (SCIs). The devastating consequences of SCIs are due to the immediate and significant disruption of the ascending and descending spinal pathways, which result in varying degrees of motor and sensory impairment. Recent therapeutic studies for SCI have focused on cell transplantation in animal models, using cells capable of inducing axon regeneration like Schwann cells (SchCs), astrocytes, genetically modified fibroblasts and olfactory ensheathing glia cells (OECs). Nevertheless, and despite the improvements in such cell-based therapeutic strategies, there is still little information regarding the mechanisms underlying the success of transplantation and regarding any secondary effects. Therefore, further studies are needed to clarify these issues. In this review, we highlight the properties of OECs that make them suitable to achieve neuroplasticity/neuroregeneration in SCI. OECs can interact with the glial scar, stimulate angiogenesis, axon outgrowth and remyelination, improving functional outcomes following lesion. Furthermore, we present evidence of the utility of cell therapy with OECs to treat SCI, both from animal models and clinical studies performed on SCI patients, providing promising results for future treatments.

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Section: Oecs In Spinal Cord Injury (Sci) Repairmentioning

confidence: 94%

Section: Om-mentioning

confidence: 97%

Cell therapy for spinal cord injury with olfactory ensheathing glia cells (OECs)

Gómez

Sanchez

Portela-Lomba

et al. 2018

Glia

Self Cite

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“…Locomotor tests using modifications of the BBB testing have been used previously as the BBB test was developed to assess spontaneous recovery from SCI and may not be ideally applied to treatments which result in recovery that deviates from the scale [48]. The addition of supplemental behavioral analyses have been reported to improve the accuracy of assessments from locomotor tests alone [49]. Therefore, we have additionally supplemented the hind end motor function tests with the addition of a toe-pinch withdrawal analysis to assess the pain sensation and the strength of the hind limb withdrawal, as well as urinary bladder dysfunction scoring.…”

Section: Neurological Testsmentioning

confidence: 99%

Local Serpin Treatment via Chitosan-Collagen Hydrogel after Spinal Cord Injury Reduces Tissue Damage and Improves Neurologic Function

Kwiecień

Zhang

Yaron

et al. 2020

JCM

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Spinal cord injury (SCI) results in massive secondary damage characterized by a prolonged inflammation with phagocytic macrophage invasion and tissue destruction. In prior work, sustained subdural infusion of anti-inflammatory compounds reduced neurological deficits and reduced pro-inflammatory cell invasion at the site of injury leading to improved outcomes. We hypothesized that implantation of a hydrogel loaded with an immune modulating biologic drug, Serp-1, for sustained delivery after crush-induced SCI would have an effective anti-inflammatory and neuroprotective effect. Rats with dorsal column SCI crush injury, implanted with physical chitosan-collagen hydrogels (CCH) had severe granulomatous infiltration at the site of the dorsal column injury, which accumulated excess edema at 28 days post-surgery. More pronounced neuroprotective changes were observed with high dose (100 µg/50 µL) Serp-1 CCH implanted rats, but not with low dose (10 µg/50 µL) Serp-1 CCH. Rats treated with Serp-1 CCH implants also had improved motor function up to 20 days with recovery of neurological deficits attributed to inhibition of inflammation-associated tissue damage. In contrast, prolonged low dose Serp-1 infusion with chitosan did not improve recovery. Intralesional implantation of hydrogel for sustained delivery of the Serp-1 immune modulating biologic offers a neuroprotective treatment of acute SCI.

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Lithium promotes recovery after spinal cord injury

et al. 2022

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Combined Strategy for a Reliable Evaluation of Spinal Cord Injury Using an in vivo Model

Abstract: In conclusion, a combined assessment strategy provides a concise method for evaluating the impact of interventions in experimental models of SCI.

Cited by 3 publications

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Cell therapy for spinal cord injury with olfactory ensheathing glia cells (OECs)

Cell therapy for spinal cord injury with olfactory ensheathing glia cells (OECs)

Local Serpin Treatment via Chitosan-Collagen Hydrogel after Spinal Cord Injury Reduces Tissue Damage and Improves Neurologic Function

Lithium promotes recovery after spinal cord injury

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