Combined selective gamma irradiation and pulverized soil inoculation for ecologically relevant soil microfauna studies

Hu, Junwei; Gebremikael, Mesfin Tsegaye; Tytgat, Bjorn; Dumack, Kenneth; Hassi, Ummehani; Hosseini, Pezhman Salehi; Sleutel, Steven; Verleyen, Elie

doi:10.1016/j.apsoil.2021.104223

Cited by 6 publications

(11 citation statements)

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“…For samples that were sterilized with gamma radiation, the results are in line with those reported by Hu, et al [38] and Luo et al [39] who tested with positive results of almost total sterility at around 25 KGy. In all cases, the efficacy of the proceeds must be checked by the absence of the microorganism's growth [40].…”

Section: Sterilize Methods Comparison For Soils: Cost Time and Effici...supporting

confidence: 90%

“…; Vol. 2, Issue 1, pp: [34][35][36][37][38][39][40]2023 different times for which the results between the three procedures were comparable. While the use of gamma radiation may be more time efficient, it can be a costly and inaccessible service for some laboratories that do not have the equipment.…”

Section: Discussionmentioning

confidence: 99%

“…Spores exhibit great resistance to different agents such as drying, freezing, thawing, high temperatures wet or dry state, UV, and gamma radiation, high pressure, and a big number of toxic chemical products like oxidation agents, aldehydes, halogens, acids and bases. Spores are more resistant than growing cells of the same specie [8], [9]. Among the most commonly used methods are sterilization by autoclaving, gamma irradiation, and fumigation with volatile chemicals such as chloroform or methyl bromide [5], [10], [11].…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, Gamma irradiation is effective and has been proposed as a selective sterilization method that can be administered in precise doses allowing the selective elimination of specific soil organisms [9]. Some authors tested that gamma irradiation produces effects on soil physical and chemical properties but is relatively less disruptive compared with other sterilization methods; effects on cation exchange capacity and pH are variable and do not show constant trends [9], [13], [14]. In this case, fungi are killed easier than bacteria, however, the treatment has shown an increase in manganese, NH 4 -N and extractable organic N but in general does not influence other physical properties [3].…”

Section: Introductionmentioning

confidence: 99%

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Sterilize Methods Comparison for Soils: Cost, Time, and Efficiency

Querejeta

2023

Int. J. Methodol.

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Soil sterilization is generally used to eliminate or reduce microbial activity in studies involving microbial inoculations, soil enzymes, among others. Achieving an adequate sterility condition is not straightforward due to the variety of resistance structures that are generated in soil microbial ecosystems and the reservoirs that can form between soil aggregates. This is why finding an effective method to achieve good sterilization is important in methodological terms, so the present work aims to compare the effectiveness of three widely used methodologies to sterilize soil and to evaluate their cost/benefit in terms of time and inputs invested. Four treatments were tested: gamma irradiation, sterilization cycles at different times: three cycles of 1 h each and four cycles of 15 min each, and chloroform vapors. The evaluation and comparison of all samples sterilized by the different methodologies were based on the total aerobic heterotrophic bacterial count. The results of this study suggest that it is more efficient to use autoclaving methods because the process is more accessible in terms of equipment and methodologies, and the final results are the same. In the case of this work, sterilization with chloroform vapors had to be rejected. While the use of gamma radiation may be more efficient in terms of time, it can be a costly and inaccessible service for some laboratories that do not have the equipment. Therefore, the most viable options in terms of time, cost, and benefit are those using autoclaves. Among these, shorter treatment times mean a reduction in the cost of using the equipment, so the option of 15-minute cycles is desirable.

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Section: Sterilize Methods Comparison For Soils: Cost Time and Effici...supporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Sterilize Methods Comparison for Soils: Cost, Time, and Efficiency

Querejeta

2023

Int. J. Methodol.

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“…gram-positive bacteria (Gram+) (iC14:0, iC15:0, aC15:0, iC16:0, aC16:0, iC17:0, aC17:0, and i19:0), gram-negative bacteria (Gram-) (cy19:0, cyC19:0, cyC17:0, cy17:0, and 18:1ω7c), undefined bacteria (C14:0, C15:0, C17:0, and C18:0) (Hu et al, 2022), actinomycetes (10MeC:16, 10Me17:0, and 10MeC:18), fungi (18:1ω9, 18:2ω6, 18:3ω3, and arbuscular mycorrhizal fungi (AMF, 16:1ω5)), and protozoa (C20:4 and C20:5) (Mendoza et al, 2022).…”

Section: Phospholipid Fatty Acid (Plfa) For Microorganism Testmentioning

confidence: 99%