Combined screening for Chlamydia trachomatis and squamous intra-epithelial lesions using a single liquid-based cervical sample

Anguenot, Jean-Luc; Marval, F. de; Vassilakos, Pierre; Auckenthaler, Raymond; Ibéchéole, Victoria; Campana, A.

doi:10.1093/humrep/16.10.2206

Cited by 4 publications

(3 citation statements)

References 24 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, molecular testing of LBP test samples has also been shown to be effective for the detection of C. trachomatis and N. Gonorrhea. [10][11][12] The results of this study indicate that such molecular testing may also be effective for the detection of T. vaginalis in LBP tests and may possibly be applied in cases in which morphology is suggestive but not diagnostic for T. vaginalis.…”

Section: Discussionmentioning

confidence: 86%

“…7 LBP also offers the advantage of the alcohol-fixed residual cells, which can be used for molecular testing of infectious organisms such as HPV, 8,9 Chlamydia trachomatis (C. trachomatis) and Neisseria gonorrhoeae (N. gonorrhoeae). [10][11][12] Recently, polymerase chain reaction (PCR) has emerged as an accurate method for detection of T. vaginalis infections not only from genital swab and vaginal tampon samples [13][14][15] but also from DNA obtained form Pap-stained CPS. 16,17 To our knowledge, no study has been performed to detect T. vaginalis DNA from residual liquid-based Pap samples.…”

mentioning

confidence: 99%

See 1 more Smart Citation

The diagnosis ofTrichomonas vaginalis in liquid-based Pap tests: Correlation with PCR

et al. 2005

View full text Add to dashboard Cite

The conventional Papanicolaou smear (CPS) is not considered accurate for the diagnosis of Trichomonas vaginalis (T. vaginalis), and women noted to carry the organism on CPS are recommended to undergo confirmatory testing. Liquid-based preparations have been shown to facilitate the diagnosis of squamous lesions and may also facilitate the diagnosis of T. vaginalis. We used polymerase chain reaction (PCR) to investigate the accuracy of the diagnosis of T. vaginalis by the liquid-based Pap test (LBP). LBP with the diagnosis of T. vaginalis from a 12-mo period were identified. Residual samples from these cases were subjected to PCR for T. vaginalis as were the residual samples of a control group of 195 LBP (including 103 inflammatory LBP and 69 cases of atypical squamous cells) in which T. vaginalis was not diagnosed cytologically. PCR confirmed the presence of T. vaginalis in 50 of 51 (98%) LBP and identified 2 additional cases. Morphologic identification of T. vaginalis on LBP is highly accurate and should not require confirmatory testing.

show abstract

Section: Discussionmentioning

confidence: 86%

mentioning

confidence: 99%

The diagnosis ofTrichomonas vaginalis in liquid-based Pap tests: Correlation with PCR

et al. 2005

View full text Add to dashboard Cite

show abstract

“…PreservCyt could be used as an alternative medium for the collection and transport of specimens for the detection of C. trachomatis, as described in published studies (Anguenot et al, 2001;Bianchi et al, 2002;Zhang et al, 2002;Koumans et al, 2003;Hopwood et al, 2004;Keegan et al, 2005;Chernesky et al, 2007), but specimens must be processed after the centrifugation step as described in this and other studies (Bianchi et al, 2002;Koumans et al, 2003). In addition, the cell pellet must be washed twice with a buffer before DNA extraction, as described by Keegan et al (2005).…”

Section: Resultsmentioning

confidence: 99%

Assessment of Chlamydia trachomatis infection by Cobas Amplicor PCR and in-house LightCycler assays using PreservCyt and 2-SP media in voluntary legal abortions

Sevestre

Mention

Lefebvre

et al. 2009

Journal of Medical Microbiology

View full text Add to dashboard Cite

Chlamydial infection of the upper genital tract after abortion is well recognized, but routine screening for infection before termination is rare, and few centres are aware of the prevalence of post-abortion complications in their patient population. Knowledge of the patient population is the best guide for developing screening strategies. The aim of this study was to determine the prevalence of chlamydial infection in patients presenting for legal termination of pregnancy, and to assess the presence of Chlamydia trachomatis by PCR on specimens collected in either PreservCyt (ThinPrep) or 2-sucrose phosphate (2-SP) transport medium. Two hundred and eleven single, sexually active women, aged 15–26 years, attending the Gynaecology and Obstetric Hospital, Amiens, France, for surgical termination of pregnancy were enrolled in this study from June 2002 to June 2003. C. trachomatis detection using a Cobas Amplicor PCR test (Roche Diagnostics) targeting a 207 bp segment of the common cryptic plasmid and a quantitative LightCycler real-time PCR (LC-PCR) (Roche Diagnostics) targeting a 123 bp fragment within the highly conserved constant domain 3 of the single-chromosome-copy ompA gene were performed on endocervical swabs in 2-SP, and on specimens collected using a cytobrush and placed in PreservCyt medium. The in-house LC-PCR was used as a chromosomal diagnosis method and to determine the load of C. trachomatis. This method was able to detect the mutant Swedish variant with a deletion of 377 bp in the target area in the cryptic plasmid, which is the region targeted by the Cobas Amplicor PCR test. C. trachomatis was detected in 19/211 patients (9 %) by both PCR methods. Among the 19 infected women, C. trachomatis was detected by the Cobas Amplicor PCR in 16 specimens in PreservCyt (7.6 %) and in 12 endocervical swabs in 2-SP (5.7 %). Specimens from only nine women were PCR-positive in both PreservCyt and 2-SP media by this method. Cobas Amplicor PCR revealed that 10.9 and 2.3 % of the PreservCyt and 2-SP samples, respectively, contained inhibitors. The same 19 infected women were LC-PCR positive in both PreservCyt and 2-SP samples. No additional infected women were found by this last method; thus, it was concluded that none of the samples contained the new variant of C. trachomatis. The load in each sample varied from 102 to 107 copies ml−1.

show abstract

Infections urogénitales féminines à Chlamydia trachomatis. Meilleures approches diagnostiques.

Henry-Suchet¹

2005

Gynécologie Obstétrique & Fertilité

View full text Add to dashboard Cite

Combined screening for Chlamydia trachomatis and squamous intra-epithelial lesions using a single liquid-based cervical sample

Abstract: We demonstrated the feasibility of combined screening for CT and SIL with a single liquid-based cervical sample.

Cited by 4 publications

References 24 publications

The diagnosis ofTrichomonas vaginalis in liquid-based Pap tests: Correlation with PCR

The diagnosis ofTrichomonas vaginalis in liquid-based Pap tests: Correlation with PCR

Assessment of Chlamydia trachomatis infection by Cobas Amplicor PCR and in-house LightCycler assays using PreservCyt and 2-SP media in voluntary legal abortions

Infections urogénitales féminines à Chlamydia trachomatis. Meilleures approches diagnostiques.

Contact Info

Product

Resources

About