Combined Quantification and Deep Serotyping for Salmonella Risk Profiling in Broiler Flocks

Obe, Tomi; Siceloff, Amy T; Crowe, Megan G; Scott, H. M.; Shariat, Nikki

doi:10.1128/aem.02035-22

Cited by 8 publications

(4 citation statements)

References 44 publications

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“…circulating in different regions of Ukraine, there is an urgent need for continuous monitoring of antibiotic resistance on livestock farms and enterprises for the production and processing of animal products. Our research results and scientific data from other scientists and practitioners confirm the correctness of the state policy chosen by Ukraine to curb the development of antimicrobial resistance (Huyghebaert et al, 2011;Polishko et al, 2011;Salmanov, 2017;Skliar et al, 2018;Obe et al, 2023).…”

Section: Discussionsupporting

confidence: 75%

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Zoonotic and commensal bacteria from pigs with acquired antimicrobial resistance

Chechet,

Gorbatyuk,

Rublenko

et al. 2023

Regul. Mech. Biosyst.

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The growing prevalence of foodborne zoonotic infections increases the risk of emergence of microorganisms with acquired resistance to antimicrobial drugs. Such microorganisms are capable of horizontal transmission of R-plasmids of antibiotic resistance to other types of bacteria. The results of our monitoring in 2021, in accordance with the State Strategy of Ukraine to curb the development of antimicrobial resistance and reduce the risks of the formation and spread of antimicrobial-resistant strains of microorganisms, showed high resistance of enterobacteria, enterococci and Campylobacter isolated from pigs to antibiotics of various groups. Among the isolated cultures of Esherichia coli, the ability of some strains to produce extended-spectrum β-lactamases (ESBL) was found. Testing of the experimental isolates for antibiotic resistance and screening for possible production of acquired resistance enzymes was carried out by the disc diffusion method with antibiotic disks of different groups. Out of 542 samples of appendix from pigs from farms in different regions of Ukraine, 138 isolates were isolated and identified. Among them: Esherichia coli – 68 strains; Enterococcus faecalis – 57; Enterococcus faecium – 9; Salmonella spp. – 2 and Campylobacter spp. – 2. Antibiotic resistance testing showed that 10 (7.3% of the isolates) of different bacterial species remained susceptibile to all antibiotics used. The remaining 128 test isolates (93.5% of the isolates) showed antibiotic resistance. The largest number of antibiotic-resistant commensal and zoonotic bacteria was detected in pig farms of Donetsk, Kyiv, Dnipro, Kirovohrad and Chernihiv regions. Escherichia coli and Enterococcus spp. were most often isolated from pigs; in smaller quantities Salmonella spp. and Campylobacter spp. All strains of Salmonella spp. faecium showed resistance to 1 to 3 antibiotics of different groups. Among the antibiotic-resistant E. faecalis, 1 strain with resistance to vancomycin was detected. Polyantibiotic resistance was inherent in E. coli strains. Among the polyantibiotic-resistant E. coli strains, 4 strains were found and confirmed to have acquired resistance due to the production of ESBL.

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Section: Discussionsupporting

confidence: 75%

“…The World Health Organization (WHO, 2017) states that antimicrobial resistance can significantly reduce global health, hinder sustainable development, and negatively affect the global well-being of humanity as a whole (Salmanov, 2017;Skliar et al, 2018;Harkavenko et al, 2020;Obe et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Zoonotic and commensal bacteria from pigs with acquired antimicrobial resistance

Chechet,

Gorbatyuk,

Rublenko

et al. 2023

Regul. Mech. Biosyst.

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“…The European Union One Health 2021 Zoonoses Report also confirms that the first and second most reported zoonoses in humans were, respectively, campylobacteriosis (accountable for >62% of confirmed human cases) and salmonellosis (accountable for >29% of confirmed human cases) [3]. While poultry production has continued to increase globally in the past few decades, the ability to effectively control persistent Salmonella populations during poultry production and processing has continued to be challenging [4][5][6][7]. Antimicrobial agents, including disinfectants and sanitizers, are commonly used directly or indirectly on products and processing surfaces to control naturally occurring bacterial pathogens and limit contamination.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Tolerance in Salmonella: Contributions to Survival and Persistence in Processing Environments

Obe,

Kiess,

Nannapaneni

2024

Animals

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Salmonella remains a top bacterial pathogen implicated in several food-borne outbreaks, despite the use of antimicrobials and sanitizers during production and processing. While these chemicals have been effective, Salmonella has shown the ability to survive and persist in poultry processing environments. This can be credited to its microbial ability to adapt and develop/acquire tolerance and/or resistance to different antimicrobial agents including oxidizers, acids (organic and inorganic), phenols, and surfactants. Moreover, there are several factors in processing environments that can limit the efficacy of these antimicrobials, thus allowing survival and persistence. This mini-review examines the antimicrobial activity of common disinfectants/sanitizers used in poultry processing environments and the ability of Salmonella to respond with innate or acquired tolerance and survive exposure to persists in such environments. Instead of relying on a single antimicrobial agent, the right combination of different disinfectants needs to be developed to target multiple pathways within Salmonella.

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“…While some serovars are most typically found in a small number of reservoirs (e.g., serovar Enteritidis is most closely linked to poultry), others, such as serovar Typhimurium, are ubiquitous and found in a variety of different hosts. Further, Salmonella is often detected in food animal production systems and the environment as mixed populations of multiple serovars (Deaven et al, 2021;Siceloff et al, 2021Siceloff et al, , 2022Obe et al, 2023). In some instances, low frequency serovars in these populations may have greater potential impacts on public health when they have clinically relevant antimicrobial resistance profiles (Siceloff et al, 2022) or are more often associated with human illness (Deaven et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and molecular characterization of Salmonella isolated from wild birds in fresh produce environments

Smith,

Varriano,

Roach

et al. 2023

Front. Microbiol.

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Wild birds pose a difficult food safety risk to manage because they can avoid traditional wildlife mitigation strategies, such as fences. Birds often use agricultural fields and structures as foraging and nesting areas, which can lead to defecation on crops and subsequent transfer of foodborne pathogens. To assess the food safety risk associated with these events, wild bird feces were collected from produce fields across the southeastern United States during the 2021 and 2022 growing seasons. In total 773 fecal samples were collected from 45 farms across Florida, Georgia, South Carolina, and Tennessee, and 2.1% (n = 16) of samples were Salmonella-positive. Importantly, 75% of Salmonella were isolated from moist feces, showing reduced Salmonella viability when feces dry out. 16S microbiome analysis showed that presence of culturable Salmonella in moist feces correlated to a higher proportion of the Enterobacteriaceae family. From the Salmonella-positive samples, 62.5% (10/16) contained multi-serovar Salmonella populations. Overall, 13 serovars were detected, including six most commonly attributed to human illness (Enteriditis, Newport, Typhimurium, Infantis, Saintpaul, and Muenchen). PCR screening identified an additional 59 Salmonella-positive fecal samples, which were distributed across moist (n = 44) and dried feces (n = 15). On-farm point counts and molecular identification from fecal samples identified 57 bird species, including for 10 Salmonella-positive fecal samples. Overall, there was a low prevalence of Salmonella in fecal samples, especially in dried feces, and we found no evidence of Salmonella transmission to proximal foliage or produce. Fecal samples collected in farms close together shared highly related isolates by whole genome sequencing and also had highly similar Salmonella populations with comparable relative frequencies of the same serovars, suggesting the birds acquired Salmonella from a common source.

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Combined Quantification and Deep Serotyping for Salmonella Risk Profiling in Broiler Flocks

Cited by 8 publications

References 44 publications

Zoonotic and commensal bacteria from pigs with acquired antimicrobial resistance

Zoonotic and commensal bacteria from pigs with acquired antimicrobial resistance

Antimicrobial Tolerance in Salmonella: Contributions to Survival and Persistence in Processing Environments

Prevalence and molecular characterization of Salmonella isolated from wild birds in fresh produce environments

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