Objectives: LumenIQ's ImageIQ software enhances conventional radiographs by adding 3-D depth. This study evaluated whether a preference exists for ImageIQ format versus conventional radiographs, determined whether ImageIQ format is as sensitive as 2-D radiographs when detecting existing periodontal defects and simulated periodontal defects. Methods: 34 dental practitioners compared 12 radiographic cases with ImageIQ format. A panel of evaluators classified periodontal defects from 2-D radiographs and ImageIQ images. Simulated periodontal defects were radiographed with various radiographic variables altered, converted to ImageIQ 3-D format, and measured to evaluate the ImageIQ format compared to the 2-D control. Results: ImageIQ format showed improved image clarity and detail over the 2-D. Changes in x-ray beam exposure time, beam angulation, and bone quality had no effect on the ImageIQ format. ImageIQ format improved the accuracy of periodontal defect classification by 14.3% over 2-D. Conclusions: ImageIQ may provide a reliable diagnostic alternative to conventional two-dimensional radiographs. Dr. Peter Ngan, for all that he brings to this program, encouraging us to learn all disciplines in this profession, for making this program fun and exciting to come to everyday. Dr. Chris Martin, for being a wonderful clinic director and friend, easy to approach and easy to discuss issues with, you make the clinic a learning atmosphere that can not be compared. Drs. Reed, Kao, Razmus, and Crout, for your unique insight and ideas on my project and for spending the individual time away from work to help me with my thesis. Drs. Hornbrook, Veselicky, and Keys, for so graciously giving your precious time to be evaluators on an integral part of my thesis. Dr. Stan Wearden, for you tireless statistical help and willingness to rerun the numbers many times.