Combined optical tomographic and magnetic resonance imaging of tumor bearing mice

Masciotti, James M.; Abdoulaev, Gassan S.; Hur, Junho K.; Papa, Joey; Bae, Jae-O; Huang, Jianzhong; Yamashiro, D.; Kandel, Jessica J.; Hielscher, Andreas H.

doi:10.1117/12.590844

Cited by 4 publications

(5 citation statements)

References 17 publications

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“…We have previously shown that optical tomography can be used to monitor the efficacy of cancer drugs in small animals 16 . In this study we show 2D axial cross-section reconstructed absorption coefficient maps which we suggest are proportional to the blood volume in the tumor.…”

Section: Optical Tomographic Imagingmentioning

confidence: 99%

“…One promising application is the monitoring of hemodynamic effects during cancer treatment in mouse models. For example, we have used optical tomography combined with MRI to monitor regression of tumors in mice after treatment with a vascular endothelia growth factor (VEGF) antagonist 16 . We found that reduction in blood volume can be observed as early as 24 hours after treatment, when anatomical changes are not yet visible in MR images.…”

Section: Introductionmentioning

confidence: 99%

“…Maps of the absorption coefficient µ a (cm -1 ) for (a) 0, (b) 4, (c) 8, (d)16, and (e) 32 minutes after injection; and changes in absorption coefficient, with respect toFig. 11a, for (f) 0, (g) 4, (h) 8, (i) 16, and (j) 32 minutes after injection.…”

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confidence: 98%

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Monitoring tumor growth and treatment in small animals with magnetic resonance and optical tomographic imaging

et al. 2006

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Small animal models are employed to simulate disease in humans and to study its progression, what factors are important to the disease process, and to study the disease treatment. Biomedical imaging modalities such as magnetic resonance imaging (MRI) and Optical Tomography make it possible to non-invasively monitor the progression of diseases in living small animals and study the efficacy of drugs and treatment protocols. MRI is an established imaging modality capable of obtaining high resolution anatomical images and along with contrast agents allow the studying of blood volume. Optical tomography, on the other hand, is an emerging imaging modality, which, while much lower in spatial resolution, can separate the effects of oxyhemoglobin, deoxyhemoglobin, and blood volume with high temporal resolution. In this study we apply these modalities to imaging the growth of kidney tumors and then there treatment by an anti-VEGF agent. We illustrate how these imaging modalities have their individual uses, but can still supplement each other and cross validation can be performed.

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Section: Optical Tomographic Imagingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Monitoring tumor growth and treatment in small animals with magnetic resonance and optical tomographic imaging

et al. 2006

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“…In this case, all sources and detectors are located on two circles defined by 1 = {(x, y, z) : x 2 + y 2 = 1.5 2 , z 1 = 2.2} and 2 = {(x, y, z) : x 2 + y 2 = 1.5 2 , z 2 = 3.5}. Similar measurement configurations are commonly used in our and other laboratories [38,44,47,69], for example, for optical measurements in finger joints, or small animal studies. For the discrete ordinate method, we use the level symmetric discrete ordinate arrangement and the corresponding weight set from [46].…”

Section: The Test Problem Set-upmentioning

confidence: 99%

Optical tomography as a PDE-constrained optimization problem

2005

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We report on the implementation of an augmented Lagrangian approach for solving the inverse problems in diffuse optical tomography (DOT). The forward model of light propagation is the radiative transport equation (RTE). The inverse problem is formulated as a minimization problem with the RTE being considered as an equality constraint on the set of 'optical properties-radiance' pairs. This approach allows the incorporation of the recently developed technique of PDE-constrained optimization, which has shown great promise in many applications that can be formulated as infinite-dimensional optimization problems. Compared to the traditional unconstrained optimization approaches for optical tomographic imaging where one solves several forward and adjoint problems at each optimization iteration, the method proposed in this work solves the forward and inverse problems simultaneously. We found in initial studies, using synthetic data, that the image reconstruction time can typically be reduced by a factor of 10 to 30, which depends on a combination of noise level, regularization parameter, mesh size, initial guess, optical properties and system geometry.

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“…The interest is motivated by advances in animal models of human diseases, and the possibility to noninvasively monitor the progression of diseases in living small animals. Different imaging techniques such as confocal imaging, 59 single-photon emission tomography, 60 MRI imaging, 61,62 fluorescence imaging, 63 and cw optical tomography 62 have been used for the detection of tumors in small animals. However, very few studies have been reported on time-domain optical imaging of animals.…”

Section: Introductionmentioning

confidence: 99%

Time-resolved optical tomography using short-pulse laser for tumor detection

et al. 2006

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Our objective is to perform a comprehensive experimental and numerical analysis of the short-pulse laser interaction with a tissue medium with the goal of tumor-cancer diagnostics. For a short-pulse laser source, the shape of the output signal is a function of the optical properties of the medium, and hence the scattered temporal optical signal helps in understanding the medium characteristics. Initially experiments are performed on tissue phantoms embedded with inhomogeneities to optimize the time-resolved optical detection scheme. Both the temporal and the spatial profiles of the scattered reflected and transmitted optical signals are compared with the numerical modeling results obtained by solving the transient radiative transport equation using the discrete ordinates technique. Next experiments are performed on in vitro rat tissue samples to characterize the interaction of light with skin layers and to validate the time-varying optical signatures with the numerical model. The numerical modeling results and the experimental measurements are in excellent agreement for the different parameters studied. The final step is to perform in vivo imaging of anesthetized rats with tumor-promoting agents injected inside skin tissues and of an anesthetized mouse with mammary tumors to demonstrate the feasibility of the technique for detecting tumors in an animal model.

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Combined optical tomographic and magnetic resonance imaging of tumor bearing mice

Cited by 4 publications

References 17 publications

Monitoring tumor growth and treatment in small animals with magnetic resonance and optical tomographic imaging

Monitoring tumor growth and treatment in small animals with magnetic resonance and optical tomographic imaging

Optical tomography as a PDE-constrained optimization problem

Time-resolved optical tomography using short-pulse laser for tumor detection

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