Combined nitrogen limitation and cadmium stress stimulate total carbohydrates, lipids, protein and amino acid accumulation in Chlorella vulgaris (Trebouxiophyceae)

Chia, Mathias Ahii; Lombardi, Ana Teresa; Melão, Maria da Graça Gama; Parrish, Christopher C.

doi:10.1016/j.aquatox.2015.01.002

Cited by 96 publications

(28 citation statements)

References 69 publications

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“…The N or P availability and the resulting N:P ratios in the media affected the toxicity of triclosan to Lemna gibba (Fulton et al, 2010(Fulton et al, , 2009, the toxicity of cadmium to Chlorella vulgaris (Chia et al, 2015), and the toxicity of ionic silver to Lemna gibba (Bian et al, 2013). Nitrogen enrichment resulting in molar TN:TP ratios of more than 150 had severe negative effects on submerged vegetation and overrode atrazine effects 6 (Dalton et al 2015).…”

Section: Introductionmentioning

confidence: 96%

Does nitrate co-pollution affect biological responses of an aquatic plant to two common herbicides?

Nuttens¹,

Chatellier

Devin

et al. 2016

Aquatic Toxicology

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Section: Introductionmentioning

confidence: 96%

Does nitrate co-pollution affect biological responses of an aquatic plant to two common herbicides?

Nuttens¹,

Chatellier

Devin

et al. 2016

Aquatic Toxicology

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“…However, most studies show that trace toxicants in wastewater have a deterrent effect on nutrient removal by algae Zhu et al 2010;Liang et al 2013), while many studies also found that significant hormesis occurred when the trace toxicant concentrations were in the range from subnanogram/L to μg/L (Calabrese 2008;Mater et al 2014;Chia et al 2015). For instance, the concentration of Cd 2+ (less than 0.1 mg/L) increased the removal efficiency of both ammonia nitrogen (NH 4 + ) and total phosphorus (TP) by increasing the release of the extracellular polymeric substances (EPS) in Chlorella vulgaris, which reduced the toxicity of Cd 2+ through the interaction of Cd 2+ with polysaccharides and protein in EPS fraction.…”

Section: Introductionmentioning

confidence: 97%

Nutrient removal by Chlorella vulgaris F1068 under cetyltrimethyl ammonium bromide induced hormesis

Zhou

et al. 2016

Environ Sci Pollut Res

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Toxicants are generally harmful to biotechnology in wastewater treatment. However, trace toxicant can induce microbial hormesis, but to date, it is still unknown how this phenomenon affects nutrient removal during municipal wastewater treatment process. Therefore, this study focused on the effects of hormesis induced by cetyltrimethyl ammonium bromide (CTAB), a representative quaternary ammonium cationic surfactant, on nutrient removal by Chlorella vulgaris F1068. Results showed that when the concentration of CTAB was less than 10 ng/L, the cellular components chlorophyll a, proteins, polysaccharides, and total lipids increased by 10.11, 58.17, 38.78, and 11.87 %, respectively, and some enzymes in nutrient metabolism of algal cells, such as glutamine synthetase (GS), acid phosphatase (ACP), H(+)-ATPase, and esterase, were also enhanced. As a result, the removal efficiencies of ammonia nitrogen (NH4 (+)) and total phosphorus (TP) increased by 14.66 and 8.51 %, respectively, compared to the control during a 7-day test period. The underlying mechanism was mainly due to an enhanced photosynthetic activity of C. vulgaris F1068 indicated by the increase in chlorophyll fluorescence parameters (the value of Fv/Fm, ΦII, Fv/Fo, and rETR increased by 12.99, 7.56, 25.59, and 8.11 %, respectively) and adenylate energy charge (AEC) (from 0.68 to 0.72). These results suggest that hormesis induced by trace toxicants could enhance the nutrient removal, which would be further considered in the design of municipal wastewater treatment processes. Graphical abstract The schematic mechanism of C. vulgaris F1068 under CTAB induced hormesis. Green arrows ( ) represent the increase and the red arrow ( ) represents the decrease.

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“…Chia et al . () also showed that the physiology of C. vulgaris could be altered under N limitation and cadmium (Cd) stress resulting in an increased lipid content reaching up to 10 pg cell −1 of total lipids in cultures with 2.9 × 10 −6 mol L −1 of N and 10 −7 mol L −1 of Cd.…”

Section: Strategies For Improvement Of Lipid Yieldsmentioning

confidence: 99%

“…Recent studies made in Chlorella pyrenoidosa have shown that when N is limited, the total lipid and neutral lipid contents exhibit the most marked increment, reaching 50.32% and 34.29% of DCW (Fan et al 2014). Chia et al (2015) also showed that the physiology of C. vulgaris could be altered under N limitation and cadmium (Cd) stress resulting in an increased lipid content reaching up to 10 pg cell −1 of total lipids in cultures with 2.9 × 10 −6 mol L −1 of N and 10 −7 mol L −1 of Cd.…”

Section: Optimization Of Culture Conditionsmentioning

confidence: 99%

Advances in culture and genetic modification approaches to lipid biosynthesis for biofuel production and in silico analysis of enzymatic dominions in proteins related to lipid biosynthesis in algae

Tamayo-Ordóñez

Ayil‐Gutiérrez

Teyer

et al. 2017

Phycological Research

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SUMMARY Biofuels have been shown to be a promising and highly attractive alternative for minimizing the use of fossil fuels, and microalgae have positioned themselves as potential candidates for production of lipids and other substances of commercial interest. We briefly review recent advances made in microalgae culture conditions and genetic manipulation for improving lipid yields for biofuel production – with both approaches showing similar yields of triacylglycerides, indicating that more work is required for improving lipid yield and accumulation in algae. Aiming at gaining knowledge of algae genetic manipulation and exploring future use of this information for modifying the lipid biosynthesis pathway, we investigated whether some characteristics of enzymes involved in lipid biosynthesis could relate to lipid yield and accumulation in algae. We made an in silico analysis of amino acid sequence of enzymatic domains and modeled tertiary structure of three proteins involved in the biosynthesis of lipids in microalgae: acetyl‐CoA carboxylase, Acyl‐CoA: diacylglycerol acyltransferase, and glycerol‐3‐phosphate acyltransferase. Our results suggest that, based on primary amino acid sequences and tertiary structure of proteins shared by certain algae, it is likely that these proteins may relate to lipid yield and accumulation, which makes bioinformatics a powerful tool for in silico study of proteins and for selecting genes involved in lipid biosynthesis that could be useful for heterologous transformation in algae with the long term objective of improving their yield, accumulation, and fatty acid composition by genetic engineering.

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Combined nitrogen limitation and cadmium stress stimulate total carbohydrates, lipids, protein and amino acid accumulation in Chlorella vulgaris (Trebouxiophyceae)

Cited by 96 publications

References 69 publications

Does nitrate co-pollution affect biological responses of an aquatic plant to two common herbicides?

Does nitrate co-pollution affect biological responses of an aquatic plant to two common herbicides?

Nutrient removal by Chlorella vulgaris F1068 under cetyltrimethyl ammonium bromide induced hormesis

Advances in culture and genetic modification approaches to lipid biosynthesis for biofuel production and in silico analysis of enzymatic dominions in proteins related to lipid biosynthesis in algae

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