Combined multiphoton imaging and biaxial tissue extension for quantitative analysis of geometric fiber organization in human reticular dermis

Ueda, Maho; Saito, Susumu; Murata, Takaho; Hirano, Toshio; Bise, Ryoma; Kabashima, Kenji; Suzuki, Shigehiko

doi:10.1038/s41598-019-47213-5

Cited by 29 publications

(25 citation statements)

References 36 publications

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“…In an elegant series of studies, Ueda et al [33] used combined MPM imaging and biaxial tissue extension to show the in vivo, 3-dimensional architecture of collagen fibre organization in the reticular dermis of men and women, with ages ranging from 36 to 75 years. The tissue was collected during reconstructive surgery.…”

Section: Collagen Fibril Formation and Characterizationmentioning

confidence: 99%

Skin collagen through the lifestages: importance for skin health and beauty

Reilly¹,

Lozano²

2021

Plast Aesthet Res

118

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Collagen-based supplements have become a keystone in the management of the ageing process, with proven ability to repair skin damage, bestowing a youthful and healthy appearance sought in the pursuit of beauty. Collagen is an essential scaffold protein that gives smoothness and elasticity to skin, but its production declines with age. Finding ways to tackle this problem is now strongly promoted as an effective way to transform skin and hair, repairing age-related deterioration. A growing number of scientific studies show exciting evidence that it is possible to rejuvenate ageing or damaged skin, improve function of worn joints, and support personal wellbeing and vitality. In recent times, research on the mechanisms which impact the production of collagen in skin and the ideal organization into functional fibres which give skin its characteristic elasticity and firmness has provided new insights into how this bio-scaffold can support cells, tissues and organs. The factors which influence collagen production over a lifetime (e.g., puberty, pregnancy, menopause, andropause), intrinsic factors (e.g., genetics, age, ethnicity) and extrinsic factors (e.g., UV-radiation, pollution, smoking) and the potential for new technologies, ingredients and devices to restore collagen and matrix components to their optimal condition are improving the ability to deliver anti-aging strategies with unprecedented results. This paper will review skin collagen production, structure and function throughout the lifestages, emphasizing its relationship with health, appearance and beauty.

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Section: Collagen Fibril Formation and Characterizationmentioning

confidence: 99%

Skin collagen through the lifestages: importance for skin health and beauty

Reilly¹,

Lozano²

2021

Plast Aesthet Res

118

View full text Add to dashboard Cite

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“…The mean cross-sectional dimensions of collagen fibers thereby increase from papillary to reticular dermis, though corresponding values vary significantly in the literature. Reported cross-sectional dimensions of fibers range from 3 to 47 μ m in the papillary dermis and from 40 to 136 μ m in the reticular dermis (Brown 1973;Ueda et al 2019;Stewart 1995;Junqueira et al 1983). In terms of collagen content, there is no sharp transition between the two layers, but rather a continuous increase in collagen content from the dermal-epidermal junction toward the reticular dermis.…”

Section: Layers Histologymentioning

confidence: 99%

A biphasic multilayer computational model of human skin

Sachs

Wahlsten

Kozerke

et al. 2021

Biomech Model Mechanobiol

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The present study investigates the layer-specific mechanical behavior of human skin. Motivated by skin’s histology, a biphasic model is proposed which differentiates between epidermis, papillary and reticular dermis, and hypodermis. Inverse analysis of ex vivo tensile and in vivo suction experiments yields mechanical parameters for each layer and predicts a stiff reticular dermis and successively softer papillary dermis, epidermis and hypodermis. Layer-specific analysis of simulations underlines the dominating role of the reticular dermis in tensile loading. Furthermore, it shows that the observed out-of-plane deflection in ex vivo tensile tests is a direct consequence of the layered structure of skin. In in vivo suction experiments, the softer upper layers strongly influence the mechanical response, whose dissipative part is determined by interstitial fluid redistribution within the tissue. Magnetic resonance imaging-based visualization of skin deformation in suction experiments confirms the deformation pattern predicted by the multilayer model, showing a consistent decrease in dermal thickness for large probe opening diameters.

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“…the Langer lines, which was confirmed ex-vivo 16,30 . However, scanning electron microscopy (SEM) and SHG ex-vivo experiments failed to support this model [35][36][37][38] .…”

Section: Resultsmentioning

confidence: 99%

General method for classification of fiber families in fiber-reinforced materials: application to in-vivo human skin images

Witte

Jaspers

Wenck

et al. 2020

Sci Rep

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Fiber structures play a major role for the function of fiber-reinforced materials such as biological tissue. An objective classification of the fiber orientations into fiber families is crucial to understand its mechanical properties. We introduce the Fiber Image Network Evaluation Algorithm (FINE algorithm) to classify and quantify the number of fiber families in scientific images. Each fiber family is characterized by an amplitude, a mean orientation, and a dispersion. A new alignment index giving the averaged fraction of aligned fibers is defined. The FINE algorithm is validated by realistic grayscale Monte-Carlo fiber images. We apply the algorithm to an in-vivo depth scan of second harmonic generation images of dermal collagen in human skin. The derived alignment index exhibits a crossover at a critical depth where two fiber families with a perpendicular orientation around the main tension line arise. This strongly suggests the presence of a transition from the papillary to the reticular dermis. Hence, the FINE algorithm provides a valuable tool for a reliable classification and a meaningful interpretation of in-vivo collagen fiber networks and general fiber reinforced materials. Biological tissue such as articular cartilage 1 , myocardium 2 , aortic valve 3 , arterial walls 4 , and skin 5 exhibit a stress strain behavior that strongly depends on the collagen fiber distribution. Fiber reinforced materials are classified by the underlying fiber network which is characterized by its anisotropy and the fiber orientation 6-8. Upon stretching, tensile forces are applied to biological specimens and collagen fibers align in the stretching direction 9-14. The characterization of the collagen network is typically determined by quantities like the orientation index, mean fiber orientation, and the fiber dispersion. These parameters are obtained from the angular orientation distribution which is commonly modeled by a pi-periodic von-Mises function 15-21. However, this approach assumes that all fibers are part of a single fiber family. Gasser et al. introduced a mechanical model for arterial walls which assumes the existence of two opposing collagen fiber families, which are oriented around a main direction 4. Parameters for this model are achieved by modeling the fiber orientation distribution using two pi-periodic von-Mises functions 22. Skin is of major relevance as it represents the largest organ of the human body. It is subject to diverse environmental stress conditions and also large mechanical strains. Langer lines, also known as cleavage lines, are reported to indicate the main orientation of collagen fibers in skin 16. We introduce the Fiber Image Networks Evaluation algorithm (FINE algorithm), which is based on the cumulative orientation distribution (COD), to classify and quantify the fiber network by means of fiber families. The FINE algorithm uses an iterative approach to identify the number of fiber families and their angular properties. The variance of the COD that is obtained by the adaptive Fourier filt...

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Combined multiphoton imaging and biaxial tissue extension for quantitative analysis of geometric fiber organization in human reticular dermis

Cited by 29 publications

References 36 publications

Skin collagen through the lifestages: importance for skin health and beauty

Skin collagen through the lifestages: importance for skin health and beauty

A biphasic multilayer computational model of human skin

General method for classification of fiber families in fiber-reinforced materials: application to in-vivo human skin images

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