Combined microfabrication and electrospinning to produce 3-D architectures for corneal repair

Ortega, Ílida; Ryan, Anthony J.; Deshpande, Pallavi; MacNeil, Sheila; Claeyssens, Frederik

doi:10.1016/j.actbio.2012.10.039

Cited by 93 publications

(95 citation statements)

References 38 publications

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“…The ring scaffolds were created by a combination of microstereolithography and electrospinning techniques 31 . In essence, the process can be summarized in 2 parts (i) creation of PEGDA templates by microstereolithography and (ii) electrospinning onto the templates for reproduction of the underlying PEGDA structure (in this case a microfabricated ring).…”

Section: Fabrication Of Plga Biodegradable Membranes Equipped With MImentioning

confidence: 99%

“…Many authors have reported work towards the development of artificial stem cells niches [25][26][27][28][29][30] . This group has recently reported the creation of a microfabricated PEGDA fibronectin-biofunctionalized artificial limbus for the delivery of limbal epithelial cells 22 and a methodology for the fabrication of electrospun biodegradable membranes containing microfabricated pockets for the support of limbal epithelial cells 31 .…”

Section: Introductionmentioning

confidence: 99%

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Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Ortega

Sefat

Deshpande

et al. 2014

JoVE

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Corneal problems affect millions of people worldwide reducing their quality of life significantly. Corneal disease can be caused by illnesses such as Aniridia or Steven Johnson Syndrome as well as by external factors such as chemical burns or radiation. Current treatments are (i) the use of corneal grafts and (ii) the use of stem cell expanded in the laboratory and delivered on carriers (e.g., amniotic membrane); these treatments are relatively successful but unfortunately they can fail after 3-5 years. There is a need to design and manufacture new corneal biomaterial devices able to mimic in detail the physiological environment where stem cells reside in the cornea. Limbal stem cells are located in the limbus (circular area between cornea and sclera) in specific niches known as the Palisades of Vogt. In this work we have developed a new platform technology which combines two cutting-edge manufacturing techniques (microstereolithography and electrospinning) for the fabrication of corneal membranes that mimic to a certain extent the limbus. Our membranes contain artificial micropockets which aim to provide cells with protection as the Palisades of Vogt do in the eye.

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Section: Fabrication Of Plga Biodegradable Membranes Equipped With MImentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Ortega

Sefat

Deshpande

et al. 2014

JoVE

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“…Since the emergence of additive manufacturing, the production of scaffolds with more complex shapes, e.g., in a specific bioreactor or to engineer an advanced tissue construct, has been a rich research field, and currently different technologies have been reported for production of biomaterial scaffolds with complex or custom shapes and hierarchical porosity. For example, additive manufacturing can be used in combination with electrospinning to produce 3D porous structures for tissue engineering [18,19] . Additionally, indirect additive manufacturing, where a 3D structure of a sacrificial material is printed and subsequently a porogen-containing material is cast in the voids.…”

Section: Introductionmentioning

confidence: 99%

Osteosarcoma growth on trabecular bone mimicking structures manufactured via laser direct write

Malayeri¹,

Sherborne²,

Paterson³

et al. 2024

IJB

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This paper describes the direct laser write of a photocurable acrylate-based PolyHIPE (High Internal Phase Emulsion) to produce scaffolds with both macro-and microporosity, and the use of these scaffolds in osteosarcoma-based 3D cell culture. The macroporosity was introduced via the application of stereolithography to produce a classical "woodpile" structure with struts having an approximate diameter of 200 μm and pores were typically around 500 μm in diameter. The PolyHIPE retained its microporosity after stereolithographic manufacture, with a range of pore sizes typically between 10 and 60 μm (with most pores between 20 and 30 μm). The resulting scaffolds were suitable substrates for further modification using acrylic acid plasma polymerisation. This scaffold was used as a structural mimic of the trabecular bone and in vitro determination of biocompatibility using cultured bone cells (MG63) demonstrated that cells were able to colonise all materials tested, with evidence that acrylic acid plasma polymerisation improved biocompatibility in the long term. The osteosarcoma cell culture on the 3D printed scaffold exhibits different growth behaviour than observed on tissue culture plastic or a flat disk of the porous material; tumour spheroids are observed on parts of the scaffolds. The growth of these spheroids indicates that the osteosarcoma behave more akin to in vivo in this 3D mimic of trabecular bone. It was concluded that PolyHIPEs represent versatile biomaterial systems with considerable potential for the manufacture of complex devices or scaffolds for regenerative medicine. In particular, the possibility to readily mimic the hierarchical structure of native tissue enables opportunities to build in vitro models closely resembling tumour tissue.

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“…[1][2][3][4][5][6] Biomimetic, nonwoven, nanofibrous scaffolds composed of large networks of interconnected fibers and pores may be fabricated via electrospinning. 7 Electrospun nanofibrous scaffolds of synthetic and natural polymers, such as polycaprolactone (PCL) and gelatin, 8 have been fabricated to regenerate organs or tissues, including bone, 9 cartilage, 10 skin, 11 neurons, 12 and blood vessels.…”

Section: Introductionmentioning

confidence: 99%

Thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells

Hong¹,

Bang²,

Xu³

et al. 2015

IJN

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Controlling the thickness of an electrospun nanofibrous scaffold by altering its pore size has been shown to regulate cell behaviors such as cell infiltration into a three-dimensional (3D) scaffold. This is of great importance when manufacturing tissue-engineering scaffolds using an electrospinning process. In this study, we report the development of a novel process whereby additional aluminum foil layers were applied to the accumulated electrospun fibers of an existing aluminum foil collector, effectively reducing the incidence of charge buildup. Using this process, we fabricated an electrospun scaffold with a large pore (pore size >40 μm) while simultaneously controlling the thickness. We demonstrate that the large pore size triggered rapid infiltration (160 μm in 4 hours of cell culture) of individual endothelial progenitor cells (EPCs) and rapid cell colonization after seeding EPC spheroids. We confirmed that the 3D, but not two-dimensional, scaffold structures regulated tubular structure formation by the EPCs. Thus, incorporation of stem cells into a highly porous 3D scaffold with tunable thickness has implications for the regeneration of vascularized thick tissues and cardiac patch development.

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Combined microfabrication and electrospinning to produce 3-D architectures for corneal repair

Cited by 93 publications

References 38 publications

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Osteosarcoma growth on trabecular bone mimicking structures manufactured via laser direct write

Thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells

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Combined microfabrication and electrospinning to produce 3-D architectures for corneal repair

Cited by 93 publications

References 38 publications

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Osteosarcoma growth on trabecular bone mimicking structures manufactured via laser direct write

Thickness-controllable electrospun fibers promote tubular structure formation by&nbsp;endothelial progenitor cells

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Thickness-controllable electrospun fibers promote tubular structure formation by endothelial progenitor cells