Combined inhibitory effect of lactoferrin and lactoperoxidase system on the viability of <i>Streptococcus mutans</i>, serotype c

Soukka, Tero; Lumikari, M; Tenovuo, Jorma

doi:10.1111/j.1600-0722.1991.tb01046.x

Cited by 28 publications

(26 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, antagonistic interactions between IgA and lactoferrin have been reported by Cole et al (1976) and Arnold et al (1984). Elevated antibacterial activity has been reported for combinations of lactoferrin and the peroxidase system (Tenovuo et al, 1982a;Moldoveanu et al, 1983;Soukka et al, 1991). Similar synergistic interactions between the peroxidase system and LZ have also been demonstrated (Goodman et al, 1981;Arnold et al, 1984).…”

mentioning

confidence: 71%

Lysozyme Enhances the Inhibitory Effects of the Peroxidase System on Glucose Metabolism of Streptococcus mutans

1992

View full text Add to dashboard Cite

The combined effect ofthe salivary peroxidase system and lysozyme on the glucose uptake of Streptococcus mutans NCTC 10449 was investigated. The bacteria were grown to late-exponential phase, washed, re-suspended in buffer at pH 6, and incubated with (1) 50 pg/ mL lysozyme from human milk for 60 min; (2) 7-15 gmol/L hypothiocyanous acid/hypothiocyanite for 10 min; and (3) lysozyme for 60 min prior to addition ofand incubation with hypothiocyanous acid/hypothiocyanite for 10 min. Glucose uptake was initiated by adding the bacterial suspensions to 10 mL ofpre-warmed 50 pmol/L glucose containing 0.98 pimol/L D-(U-"4C-)-glucose, and the mixture was incubated in a shaking water-bath at 370C. Samples were withdrawn at various time intervals, rapidly filtered through 0.45-pm membranes, washed with ice-chilled buffer, and the incorporated radioactivity determined. Lysozyme stimulated S. mutans glucose uptake slightly, but significantly inhibited S. rattus glucose metabolism. A 20-30% inhibition ofradiolabeled glucose incorporation was observed with hypothiocyanous acid/hypothiocyanite alone. Incubationofthe bacteriawithlysozyme priortoaddition ofhypothiocyanous acid/hypothiocyanite containingperoxidase resulted in a total inhibition ofthe glucose uptake. In contrast, lysozyme in combination with hypothiocyanous acid/hypothiocyanite without peroxidase gave only a 30-50% inhibition. The addition of 5 mmol/L dithiothreitol after incubation withlysozyme and hypothiocyanous acid/hypothiocyanite eliminated the inhibition of the bacterial glucose uptake. The viability ofS. mutans was not affected by treatment with any ofthe components used. Our results indicate that physiological concentrations of lysozyme and the salivary peroxidase system components have a synergistic effect which results in a significant inhibition of glucose metabolism by S. mutans. J Dent Res 71 (3): 484-490, March, 1992

show abstract

mentioning

confidence: 71%

Lysozyme Enhances the Inhibitory Effects of the Peroxidase System on Glucose Metabolism of Streptococcus mutans

1992

View full text Add to dashboard Cite

show abstract

“…1992b] or lactoferrin [Arnold et al, 1984;Soukka et al. 1991] enhances the antibacterial effect of the LPO system against S. mutans.…”

Section: Discussionmentioning

confidence: 99%

Effects of a Lactoperoxidase System-Containing Toothpaste on Levels of Hypothiocyanite and Bacteria in Saliva

1993

View full text Add to dashboard Cite

Lactoperoxidase (LPO)/H₂O₂/SCN^--system-generated hypothiocyanite ions (OSCN^-) and hypothiocyanous acid (HOSCN) are inhibitory against a number of oral bacteria, including mutans streptococci. A commercially available toothpaste (Biotene®) comprises the complete LPO system. Generation of HOSCN/OSCN^–– by Biotene was studied in vitro both in sterilized and nonsterilized saliva of 10 healthy subjects. The HOSCN/OSCN^–– yield ranged from 100 to 300 μM with Biotene, while the salivary levels of HOSCN/OSCN^–– before the addition of Biotene were 30.1 ± 25.1 μM. Two in vivo trials were carried out-In the first study, resting saliva was collected from 12 individuals before immediately after, and 2,5 10 and 20 min after brushing with Biotene to evaluate the in vivo generation and decomposition of HOSCN/OSCN^-. In the second study, 26 healthy individuals attended a 1-month crossover trial with Biotene and a control toothpaste, Vademecum® (no LPO system), both containing F^- and xylitol. The salivary counts of total streptococci, mutans streptococci (MS), lactobacilli and the total flora (TF), as well as the peroxidase, thiocya-nate ion and HOSCN/OSCN^- levels were determined before and after 2 and 4 weeks daily use of the toothpastes. Twice-a-day use of Biotene for 1 month resulted in an elevation of ‘resting levels’ of HOSCN/OSCN^-. No such effect was found with the control toothpaste. No significant changes were found in the salivary levels of total streptococci, MS, lactobacilli or TF after 1-month use of either toothpaste. The results show the capability of the LPO-system-containing toothpaste to elevate the salivary levels of HOSCN/OSCN^-, although no bactericidal effect was observed.

show abstract

“…Consumption of yoghurt might reduce biofilm formation on indwelling prostheses, due to the presence of Streptococcus thermophilus and Lactobacillus (Busscher et al, 1997b;Havenaar & Huis in't Veld, 1992). Buttermilk is rich in lactoferrin, which has bactericidal and fungicidal activity against a number of oral pathogenic organisms, including C. albicans, Aggregatibacter actinomycetemcomitans and Streptococcus mutans (Holmes et al, 2012;Kalmar & Arnold, 1988;Soukka et al, 1991Soukka et al, , 1992. This may explain the relatively prolonged lifetime of the prostheses and the varied microbial colonization in this study.…”

Section: Microbial Composition Of Biofilms On Voice Prosthesesmentioning

confidence: 99%

Candida biofilm formation on voice prostheses

Talpaert

Balfour

Stevens

et al. 2015

Journal of Medical Microbiology

View full text Add to dashboard Cite

Combined inhibitory effect of lactoferrin and lactoperoxidase system on the viability of Streptococcus mutans, serotype c

Cited by 28 publications

References 22 publications

Lysozyme Enhances the Inhibitory Effects of the Peroxidase System on Glucose Metabolism of Streptococcus mutans

Lysozyme Enhances the Inhibitory Effects of the Peroxidase System on Glucose Metabolism of Streptococcus mutans

Effects of a Lactoperoxidase System-Containing Toothpaste on Levels of Hypothiocyanite and Bacteria in Saliva

Candida biofilm formation on voice prostheses

Contact Info

Product

Resources

About