Combined histochemical approach in assessing tryptase expression in the mast cell population

Atiakshin, Dmitri; Шишкина, В. В.; Герасимова, О. А.; Meshkova, V.Y.; Samodurova, N. Yu.; Самойленко, Т. В.; Buchwalow, Igor; Samoilova, Vera; Tiemann, Markus

doi:10.1016/j.acthis.2021.151711

Cited by 9 publications

(8 citation statements)

References 12 publications

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“…In this case, a complex of serglycine with heparin or chondroitin sulfate accumulates in the center of the granule [90]. Morphological evidence of this fact is the smaller size of metachromatic MC granules after histochemical staining, for example, with toluidine blue, in comparison with the immunomorphological detection of specific mast cell proteases [91]. It should be noted that serglycine is a necessary proteoglycan of the granule for the subsequent folding of specific MC proteases, as well as histamine and serotonin.…”

Section: Biogenesis Of Cpa3-containing Granulesmentioning

confidence: 99%

“…In particular, in experiments on rats using a combined histochemical protocol, we have shown that a large number of mast cells in the skin can be detected on the basis of metachromasia. This suggests that the total pool of MCs in the organ may escape the attention of researchers if they rely only on the content of one of the specific proteases [91].…”

Section: Cytotopographic Featuresmentioning

confidence: 99%

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Carboxypeptidase A3—A Key Component of the Protease Phenotype of Mast Cells

Atiakshin

Kostin

Троценко

et al. 2022

Cells

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Carboxypeptidase A3 (CPA3) is a specific mast cell (MC) protease with variable expression. This protease is one of the preformed components of the secretome. During maturation of granules, CPA3 becomes an active enzyme with a characteristic localization determining the features of the cytological and ultrastructural phenotype of MC. CPA3 takes part in the regulation of a specific tissue microenvironment, affecting the implementation of innate immunity, the mechanisms of angiogenesis, the processes of remodeling of the extracellular matrix, etc. Characterization of CPA3 expression in MC can be used to refine the MC classification, help in a prognosis, and increase the effectiveness of targeted therapy.

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Section: Biogenesis Of Cpa3-containing Granulesmentioning

confidence: 99%

Section: Cytotopographic Featuresmentioning

confidence: 99%

Carboxypeptidase A3—A Key Component of the Protease Phenotype of Mast Cells

Atiakshin

Kostin

Троценко

et al. 2022

Cells

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“…Attention was drawn to the regularity of the rarer detection of CD38 in MCs containing well-defined granules. In mastocytosis, CD38 is more often expressed in hypochromic MCs containing small granules [25].…”

Section: Cd38 Cytotopography In Mast Cellsmentioning

confidence: 99%

Expression of CD38 in Mast Cells: Cytological and Histotopographic Features

Atiakshin

Samoilova

Buchwalow

et al. 2021

Cells

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The biological significance of the CD38 molecule goes beyond metabolic, enzymatic, and proliferative functions. CD38 possesses the functions of an exoenzyme and receptor, and is actively involved in the mechanisms of adhesion, migration, intercellular signaling, formation of immune synapses, and modulation of the activity of a wide range of immune and non-immune cells. The aim of this study was the immunohistochemical assessment of the cytological and histotopographic characteristics of CD38 expression in mast cells. CD38 expression was found in a minority of the mast cell population. It is characterized by wide variability from low to high levels. The intensity of CD38 expression in mast cells has organ-specific features and depends on the development of pathological processes in a specific tissue microenvironment. The mechanisms of intercellular interaction between mast cells and CD38+ cells foster new understanding of the protumorigenic or antitumor potential of tryptase.

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“…In particular, the study of the specific MC protease expression, tryptase, is of special significance. Using a combined histochemical approach, we have previously demonstrated increased tryptase expression in a mast cell population under various treatment protocols, primarily molecular hydrogen [ 21 , 22 , 23 ]. Tryptase has a high biological activity, affecting the state of many cellular and non-cellular components of the tissue microenvironment [ 14 , 24 , 25 , 26 , 27 , 28 , 29 ].…”

Section: Discussionmentioning

confidence: 99%

Mast Cells in Regeneration of the Skin in Burn Wound with Special Emphasis on Molecular Hydrogen Effect

et al. 2023

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The mechanisms of regeneration for the fibrous component of the connective tissue of the dermis are still insufficiently studied. The aim of this study was to evaluate the effectiveness of the use of molecular hydrogen on the local therapy of a II degree burn wound with the intensification of collagen fibrillogenesis in the skin. We analyzed the involvement of mast cells (MCs) in the regeneration of the collagen fibers of the connective tissue using water with a high content of molecular hydrogen and in a therapeutic ointment for the cell wounds. Thermal burns led to an increase in the skin MC population, accompanied by a systemic rearrangement of the extracellular matrix. The use of molecular hydrogen for the treatment of burn wounds stimulated the regeneration processes by activating the formation of the fibrous component of the dermis, accelerating wound healing. Thus, the intensification of collagen fibrillogenesis was comparable to the effects of a therapeutic ointment. The remodeling of the extracellular matrix correlated with a decrease in the area of damaged skin. Skin regeneration induced by the activation of the secretory activity of MCs may be one of the possible points of implementation of the biological effects of molecular hydrogen in the treatment of burn wounds. Thus, the positive effects of molecular hydrogen on skin repair can be used in clinical practice to increase the effectiveness of therapy after thermal exposure.

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Combined histochemical approach in assessing tryptase expression in the mast cell population

Cited by 9 publications

References 12 publications

Carboxypeptidase A3—A Key Component of the Protease Phenotype of Mast Cells

Carboxypeptidase A3—A Key Component of the Protease Phenotype of Mast Cells

Expression of CD38 in Mast Cells: Cytological and Histotopographic Features

Mast Cells in Regeneration of the Skin in Burn Wound with Special Emphasis on Molecular Hydrogen Effect

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