Combined Hind Limb Suspension and sRANK-L on Bone Strength in Mice by Finite Element Analysis: Effects of Anatomic Model Height

Hezrony, Benjamin S.; Speacht, Toni L.; Donahue, Henry J.; Lau, Anthony G.

doi:10.1109/embc44109.2020.9175729

Cited by 1 publication

(1 citation statement)

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“…Studies have shown 21,24,25 that the number of bone trabeculae decreases and bone spacing increases during long-term weightlessness or bed rest among humans 26 . Currently, weightlessness studies are mainly selected by relying on animal models 27,28 , and the rodent hindlimb unloading model is the most widely used 29,30 to study spatial effects, of which, bone loss and metabolic changes are the most signi cant. However, this model has certain limitations in that it can only be applied locally and not to the whole experimental animal 31 .…”

Section: Introductionmentioning

confidence: 99%

Temporal effects of three-dimensional clinostat treatment on bone and serum metabolome in C57BL/J mice: a new method of simulating microgravity effect

Song

Kang

Zhang

et al. 2022

Preprint

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Microgravity is an unavoidable aspect of space flight. A three-dimensional (3D) clinostat is a great method to simulate the effects of microgravity on Earth. The impact of microgravity on plants, cells, and Caenorhabditis elegans has been extensively studied. However, no study has used a 3D clinostat apparatus to simulate the effects of microgravity on mouse models. Therefore, we conducted a study to produce a space microgravity mouse models using 3D clinostat treatment and explored the time effect of 3D clinostat treatment on the skeleton and metabolome of C57BL/J mice. Thirty 8-week-old male C57BL/J mice were randomly assigned to three groups: mice in individually ventilated cages (MC group, n = 6), mice in survival boxes (SB group, n = 12), and mice in survival boxes receiving 3D clinostat treatment (CS group, n = 12). Bone loss was assessed using microcomputed tomography of the left femur, whereas the changes in serum metabolites were monitored using untargeted metabolomics. A marked reduction in the trabecular number (p < 0.05) and an increased trabecular spacing (p < 0.1) were observed to occur in a time-dependent manner in the CS group compared with the SB group. Compared with the metabolome of the SB group, the metabolome of the CS group showed significant differences at Ⅰ and Ⅳ stages. Furthermore, the common pathways involved in differential metabolites in the Ⅰ and Ⅳ stages were valine, leucine, isoleucine degradation, and 2-oxocarboxylic acid metabolism. The KEGG pathways in the Ⅳ stages were mainly related to the nervous system, which was a result of microgravity.

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