Combined high-fat diet and sustained high sucrose consumption promotes NAFLD in a murine model

Torres‐Villalobos, Gonzalo; Hamdan‐Pérez, Nashla; Tovar, Armando R.; Ordaz-Nava, Guillermo; Martínez‐Benitez, Braulio; Torre‐Villalvazo, Ivan; Morán-Ramos, Sofía; Dı́az-Villaseñor, Andrea; Noriega, Lilia G.; Hiriart, Marcia; Medina-Santillán, Roberto; Castillo-Hernández, María del Carmen; Méndez-Sánchez, Nahúm; Uribe, Misael; Torres, Nimbe

doi:10.1016/s1665-2681(19)31176-7

Cited by 29 publications

(32 citation statements)

References 21 publications

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“…Many studies have reported that intake of sucrose solution can induce abnormal metabolic status, including high levels of blood triglyceride, glucose and insulin, leading to body weight gain in human2954 and animal models27555657. However, in the present study, there were no significant changes in the levels of glucose, insulin, or triglycerides in the blood of S30 mice as compared to CT or A30 mice (Supplemental Fig.…”

Section: Discussioncontrasting

confidence: 64%

“…This discrepancy might be generated, at least in part, by the fact that different experimental designs, such as experimental diet, experimental period, and animal strains have been applied. The metabolic effects of sucrose consumption examined together with a high fat diet might be different from those associated with a normal fat diet57. In addition, the initiating time point in the life cycle and duration of the sucrose challenge might be important contributing factors275663.…”

Section: Discussionmentioning

confidence: 99%

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Long-term consumption of sugar-sweetened beverage during the growth period promotes social aggression in adult mice with proinflammatory responses in the brain

Choi

Park

Kim

et al. 2017

Sci Rep

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Overconsumption of sugar-sweetened beverages (SSBs) is known to be a key contributor to the obesity epidemic; however, its effects on behavioral changes are yet to be fully studied. In the present study, we examined the long-term effects of SSB on social aggression in mice. Three-week-old weaned mice started to drink either a 30 w/v% sucrose solution (S30), plain water (CT), or an aspartame solution with sweetness equivalent to the sucrose solution (A30) and continued to drink until they were 11-week-old adults. Aggressive behaviors were assessed by the resident-intruder test. We found that SSB significantly promoted social aggression, accompanied by heightened serum corticosterone and reduced body weight. To understand the underlying mechanism, we performed transcriptome analyses of brain. The profiles of mice on S30 were dramatically different from those on CT or A30. Transcriptional networks related to immunological function were significantly dysregulated by SSB. FACS analysis of mice on S30 revealed increased numbers of inflammatory cells in peripheral blood. Interestingly, the artificial sweetener failed to mimic the effects of sugar on social aggression and inflammatory responses. These results demonstrate that SSB promotes aggressive behaviors and provide evidence that sugar reduction strategies may be useful in efforts to prevent social aggression.

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Section: Discussioncontrasting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Long-term consumption of sugar-sweetened beverage during the growth period promotes social aggression in adult mice with proinflammatory responses in the brain

Choi

Park

Kim

et al. 2017

Sci Rep

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“…; Torres‐Villalobos et al. ). Therefore, to assess the impact of an autologous fat transplant from the subcutaneous fat pad to the visceral compartments, we developed a diet‐induced obesity (DIO) using a HFD or HCHD.…”

Section: Resultsmentioning

confidence: 99%

“…; Torres‐Villalobos et al. ). However, whether an adipose tissue transplant can provide the same health benefits in obese animals depending on whether they developed obesity with a HFD or HCHD is unknown.…”

Section: Introductionmentioning

confidence: 99%

Autologous subcutaneous adipose tissue transplants improve adipose tissue metabolism and reduce insulin resistance and fatty liver in diet-induced obesity rats

et al. 2016

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Long‐term dietary and pharmacological treatments for obesity have been questioned, particularly in individuals with severe obesity, so a new approach may involve adipose tissue transplants, particularly autologous transplants. Thus, the aim of this study was to evaluate the metabolic effects of autologous subcutaneous adipose tissue (SAT) transplants into two specific intraabdominal cavity sites (omental and retroperitoneal) after 90 days. The study was performed using two different diet‐induced obesity (DIO) rat models: one using a high‐fat diet (HFD) and the other using a high‐carbohydrate diet (HCHD). Autologous SAT transplant reduced hypertrophic adipocytes, improved insulin sensitivity, reduced hepatic lipid content, and fasting serum‐free fatty acids (FFAs) concentrations in the two DIO models. In addition, the reductions in FFAs and glycerol were accompanied by a greater reduction in lipolysis, assessed via the phosphorylation status of HSL, in the transplanted adipose tissue localized in the omentum compared with that localized in the retroperitoneal compartment. Therefore, the improvement in hepatic lipid content after autologous SAT transplant may be partially attributed to a reduction in lipolysis in the transplanted adipose tissue in the omentum due to the direct drainage of FFAs into the liver. The HCHD resulted in elevated fasting and postprandial serum insulin levels, which were dramatically reduced by the autologous SAT transplant. In conclusion, the specific intraabdominal localization of the autologous SAT transplant improved the carbohydrate and lipid metabolism of adipose tissue in obese rats and selectively corrected the metabolic parameters that are dependent on the type of diet used to generate the DIO model.

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“…Also, upregulated miR-21 levels in serum are associated with nonalcoholic fatty liver disease, especially in men [20]. Accordingly, 20% consumption of sucrose has been reported associated with mild liver steatosis in rats [21]. This miRNA may have a role in sustaining adipose tissue expansion as reported in a study using miR-21 antagomiRs in the db/db mice [22].…”

Section: Discussionmentioning

confidence: 99%

Inflammation Related MicroRNAs Are Modulated in Total Plasma and in Extracellular Vesicles from Rats with Chronic Ingestion of Sucrose

Brianza-Padilla

Carbó

Arana

et al. 2016

BioMed Research International

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Circulating microRNAs (miRNAs) and the functional implications of miRNAs contained in extracellular vesicles (EVs) have gained attention in the last decade. Little is known about the regulation of the abundance of plasma miRNAs in response to chronic ingestion of carbohydrates. Therefore, we explored the circulating levels of miR-21, miR-146a, miR-155, and miR-223 in rats consuming sucrose in drinking water. Weanling Wistar rats were 25 weeks with 30% sucrose in drinking water, and miRNAs expression was determined in total plasma and in microvesicles, by RT-qPCR with TaqMan probe based assays for miR-21, miR-146a, miR-155, and miR-223, using cel-miR-39 (as spike in control and reference). Endotoxemia was also measured. Sucrose-fed animals showed higher body weight and retroperitoneal adipose tissue as well as higher glucose and triglyceride plasma levels than controls. Plasma endotoxin levels were low and not different among groups. Plasma miR-21 and miR-223 were higher in the sucrose group (p < 0.05), whereas miR-155 tended to be lower (p = 0.0661), and miR-146a did not show significant differences. In the plasma EVs the same trend was found except for miR-146a that showed significantly higher levels (p < 0.05). Overall, our results show that high carbohydrate ingestion modulates circulating miRNAs levels related to an inflammatory response.

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Combined high-fat diet and sustained high sucrose consumption promotes NAFLD in a murine model

Cited by 29 publications

References 21 publications

Long-term consumption of sugar-sweetened beverage during the growth period promotes social aggression in adult mice with proinflammatory responses in the brain

Long-term consumption of sugar-sweetened beverage during the growth period promotes social aggression in adult mice with proinflammatory responses in the brain

Autologous subcutaneous adipose tissue transplants improve adipose tissue metabolism and reduce insulin resistance and fatty liver in diet-induced obesity rats

Inflammation Related MicroRNAs Are Modulated in Total Plasma and in Extracellular Vesicles from Rats with Chronic Ingestion of Sucrose

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