Combined genetic and bioactivity‐based prioritization leads to the isolation of an endophyte‐derived antimycobacterial compound

Alvin, Alfonsus; Kalaitzis, John A.; Sasia, B.; Neilan, Brett A.

doi:10.1111/jam.13062

Cited by 15 publications

(7 citation statements)

References 62 publications

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“…Four new compounds, 14-hydroxytajixanthone (125), 14-hydroxyltajixanthone hydrate (126, Figure 7), 14-hydroxy-15-chlorotajixanthone hydrate (127) and epitajixanthone hydrate (128), along with known compounds tajixanthone hydrate (129), 14-methoxyltajixanthone-25-acetate (130), and 15-chlorotajixanthone hydrate (131), questin (132) An endophytic fungus Byssochlamys spectabilis from the plant Edgeworthia chrysantha yielded bysspectin C (134, Figure 8) which was active against E. coli and S. aureus with MIC values of 32 and 64 µg/mL, respectively [75].…”

Section: Minor Taxa Of the Ascomycetesmentioning

confidence: 99%

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Fungal Endophytes: A Potential Source of Antibacterial Compounds

Deshmukh

Dufossé²,

Chhipa

et al. 2022

JoF

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Antibiotic resistance is becoming a burning issue due to the frequent use of antibiotics for curing common bacterial infections, indicating that we are running out of effective antibiotics. This has been more obvious during recent corona pandemics. Similarly, enhancement of antimicrobial resistance (AMR) is strengthening the pathogenicity and virulence of infectious microbes. Endophytes have shown expression of various new many bioactive compounds with significant biological activities. Specifically, in endophytic fungi, bioactive metabolites with unique skeletons have been identified which could be helpful in the prevention of increasing antimicrobial resistance. The major classes of metabolites reported include anthraquinone, sesquiterpenoid, chromone, xanthone, phenols, quinones, quinolone, piperazine, coumarins and cyclic peptides. In the present review, we reported 451 bioactive metabolites isolated from various groups of endophytic fungi from January 2015 to April 2021 along with their antibacterial profiling, chemical structures and mode of action. In addition, we also discussed various methods including epigenetic modifications, co-culture, and OSMAC to induce silent gene clusters for the production of noble bioactive compounds in endophytic fungi.

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Section: Minor Taxa Of the Ascomycetesmentioning

confidence: 99%

“…The polyketide javanicin (289, Figure 16) was purified from Fusarium sp. associated with Rhoeo spathacea, and displayed activity against M. tuberculosis with a MIC value of 25 µg/mL and M. phlei with a MIC value of 50 µg/mL [132].…”

Section: Fusariummentioning

confidence: 99%

Fungal Endophytes: A Potential Source of Antibacterial Compounds

Deshmukh

Dufossé²,

Chhipa

et al. 2022

JoF

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“…A molecular approach was used to prioritize endophytes with the potential to synthesize specialized metabolites (NRPs and PKs). This approach has proven useful for the discovery of microbial strains and compounds of biomedical value from terrestrial and marine environments, including medicinal plants (Burns et al 2005;Zhang et al 2009;Alvin et al 2016;Mishra et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Australian bush medicines harbour diverse microbial endophytes with broad‐spectrum antibacterial activity

Ingrey

Pearson²,

Kalaitzis³

et al. 2021

J Appl Microbiol

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Aims: Microbial endophytes produce specialized metabolites, including antibiotics and other compounds of pharmaceutical and agricultural value. This study aimed to investigate the diversity and bioactivity of endophytes from medicinal plants used by the Dharawal People of Gamay (Botany Bay), Australia. Methods and Results: Of the 48 endophytes isolated, 19 tested positive for polyketide synthase or non-ribosomal peptide synthetase genes via a PCR incorporating degenerate primers. The biosynthetically talented endophytes were identified by 16S rRNA gene sequencing and included 4 bacteria species belonging to the orders Bacillales, Rhizobiales and Burkholderiales and 15 Ascomycota fungi species belonging to the orders Botryosphaeriales, Cladosporiales, Glomerellales, Microascales and Eurotiales. Antimicrobial testing using the disc diffusion assay demonstrated that 15 of the 19 isolates had broad-spectrum activity against a range of Gram-positive and Gramnegative bacteria. Conclusions: Taken together, these results suggest that Australian bush medicines harbour diverse biosynthetically talented microbial endophytes capable of producing broad-spectrum antibacterial compounds. Significance and Impact of the Study: This study suggests that compounds produced by microbial endophytes likely contribute to the collective medicinal properties of Australian bush medicines. Significantly, it highlights that Indigenous botanical knowledge and modern molecular approaches can be used in tandem to prioritize microorganisms that produce pharmaceutically relevant compounds.

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“…The combination of two orthologous techniques proved well-adapted to the prioritisation of cyanobacterial strains for the discovery of novel compound classes. Another approach was applied to endophytes taken from various organs of medicinal plant Rhoeo spathacea 43 to identify those capable of producing bioactive antibacterial PKs and PEPs. In this study, bioactivity-based screening was used in combination with genetic screening for PKS and NRPS genes to select the most promising strain among 10 fungal endophytic strains.…”

Section: Prioritisation Procedures For the Investigation Of Microorgamentioning

confidence: 99%

Innovative omics-based approaches for prioritisation and targeted isolation of natural products – new strategies for drug discovery

et al. 2019

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Combined genetic and bioactivity‐based prioritization leads to the isolation of an endophyte‐derived antimycobacterial compound

Abstract: This is the first report on the fungal endophytic diversity of R. spathacea, and the isolation of an antimycobacterial compound from the plant which has been traditionally used for the treatment of tuberculosis symptoms.

Cited by 15 publications

References 62 publications

Fungal Endophytes: A Potential Source of Antibacterial Compounds

Fungal Endophytes: A Potential Source of Antibacterial Compounds

Australian bush medicines harbour diverse microbial endophytes with broad‐spectrum antibacterial activity

Innovative omics-based approaches for prioritisation and targeted isolation of natural products – new strategies for drug discovery

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