Combined fluorescence and reflectance spectroscopy: in vivo assessment of oral cavity epithelial neoplasia

Fuchs, H.; Utzinger, Urs; Zuluaga, Andrés F.; Gillenwater, Ann M.; Jacob, Rhonda F.; Kemp, Bonnie L.; Richards‐Kortum, Rebecca

doi:10.1109/cleo.1998.676202

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…It is interesting to note that emission spectra obtained at 400 nm excitation are included in a majority of the top combinations, suggesting that differences in absorption due to perfusion may offer diagnostic information. This suggests that the combinations of reflectance and fluorescence spectroscopy may offer improved diagnostic performance and will be the subject of future work (38). The inclusion of red fluorescence intensity information with the optimal wavelength combination did not improve algorithm performance.…”

Section: Discussionmentioning

confidence: 92%

Optimal Excitation Wavelengths for In Vivo Detection of Oral Neoplasia Using Fluorescence Spectroscopy¶

Heintzelman

Utzinger

Fuchs

et al. 2000

Photochem Photobiol

141

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There is no satisfactory mechanism to detect premalignant lesions in the upper aero-digestive tract. Fluorescence spectroscopy has potential to bridge the gap between clinical examination and invasive biopsy; however, optimal excitation wavelengths have not yet been determined. The goals of this study were to determine optimal excitation-emission wavelength combinations to discriminate normal and precancerous/cancerous tissue, and estimate the performance of algorithms based on fluorescence. Fluorescence excitation-emission matrices (EEM) were measured in vivo from 62 sites in nine normal volunteers and 11 patients with a known or suspected premalignant or malignant oral cavity lesion. Using these data as a training set, algorithms were developed based on combinations of emission spectra at various excitation wavelengths to determine which excitation wavelengths contained the most diagnostic information. A second validation set of fluorescence EEM was measured in vivo from 281 sites in 56 normal volunteers and three patients with a known or suspected premalignant or malignant oral cavity lesion. Algorithms developed in the training set were applied without change to data from the validation set to obtain an unbiased estimate of algorithm performance. Optimal excitation wavelengths for detection of oral neoplasia were 350, 380 and 400 nm. Using only a single emission wavelength of 472 nm, and 350 and 400 nm excitation, algorithm performance in the training set was 90% sensitivity and 88% specificity and in the validation set was 100% sensitivity, 98% specificity. These results suggest that fluorescence spectroscopy can provide a simple, objective tool to improve in vivo identification of oral cavity neoplasia.

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Section: Discussionmentioning

confidence: 92%

Optimal Excitation Wavelengths for In Vivo Detection of Oral Neoplasia Using Fluorescence Spectroscopy¶

Heintzelman

Utzinger

Fuchs

et al. 2000

Photochem Photobiol

141

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“…Fiber tracks are electronically binned on the CCD to detect lower light levels. 52 Lin et al 49 and Marquez and Wang 53 modified their linear probe design with an oblique incident source fiber and measured the shift of the reflectance profile. This removes the necessity of measuring in absolute units.…”

Section: Diffuse Reflectance Probesmentioning

confidence: 99%

Fiber optic probes for biomedical optical spectroscopy

2003

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Fiber optic probes are a key element for biomedical spectroscopic sensing. We review the use of fiber optic probes for optical spectroscopy, focusing on applications in turbid media, such as tissue. The design of probes for reflectance, polarized reflectance, fluorescence, and Raman spectroscopy is illustrated. We cover universal design principles as well as technologies for beam deflecting and reshaping.

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Combined fluorescence and reflectance spectroscopy: in vivo assessment of oral cavity epithelial neoplasia

Cited by 2 publications

References 5 publications

Optimal Excitation Wavelengths for In Vivo Detection of Oral Neoplasia Using Fluorescence Spectroscopy¶

Optimal Excitation Wavelengths for In Vivo Detection of Oral Neoplasia Using Fluorescence Spectroscopy¶

Fiber optic probes for biomedical optical spectroscopy

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