Combined field-induced dielectrophoresis and phase separation for manipulating particles in microfluidics

Bennett, Dawn J.; Khusid, Boris; James, Conrad D.; Galambos, Paul C.; Okandan, Murat; Jacqmin, David; Acrivos, Andreas

doi:10.1063/1.1629789

Cited by 39 publications

(48 citation statements)

References 13 publications

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“…These gates, used in a continuous flow device, can be used to direct and deflect particles to specific regions on the chip or trap the particles. 19,[21][22][23][24][25][26] However, the alignment and bonding of the top and bottom electrodes is difficult and has prevented many DEP techniques from adapting to this configuration. The aligning can be done using a microscope, however, the bonding of the chip once the electrodes are aligned creates additional difficulties.…”

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confidence: 99%

An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting

2007

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Multi-target pathogen detection using heterogeneous medical samples require continuous filtering, sorting, and trapping of debris, bioparticles, and immunocolloids within a diagnostic chip. We present an integrated AC dielectrophoretic ͑DEP͒ microfluidic platform based on planar electrodes that form three-dimensional ͑3D͒ DEP gates. This platform can continuously perform these tasks with a throughput of 3 L / min. Mixtures of latex particles, Escherichia coli Nissle, Lactobacillus, and Candida albicans are sorted and concentrated by these 3D DEP gates. Surface enhanced Raman scattering is used as an on-chip detection method on the concentrated bacteria. A processing rate of 500 bacteria was estimated when 100 l of a heterogeneous colony of 10 7 colony forming units /ml was processed in a single pass within 30 min.

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confidence: 99%

An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting

2007

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“…The principle of filtration is based on the concept of dielectrophoretic gating that we recently developed for microfluidics [19,20]. This concept combines the AC dielectrophoresis and the field-induced phase separation of a suspension into low-and high-concentrated phases for manipulating particles.…”

Section: Principle Of Filter Operationmentioning

confidence: 99%

A novel concept of dielectrophoretic engine oil filter

2011

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A novel concept of an alternating current (AC) dielectrophoretic filter with a three-dimensional electrode array is presented. A filter is constructed by winding into layers around the core tube two sheets of woven metal wire-mesh with several sheets of woven insulating wire-mesh sandwiched in between. Contrary to conventional dielectrophoretic devices, the proposed design of electrodes generates a high-gradient field over a large working volume by applying several hundred volts at a standard frequency of 60 Hz. The operating principle of filtration is based on our recently developed method of AC dielectrophoretic gating for microfluidics. The filtration efficiency is expressed in terms of two non-dimensional parameters, which describe the combined influence of the particle polarizability and size, the oil viscosity and flow rate, and the field gradient on the particle captivity. The proof-of-concept is tested by measuring the single-pass performance of two filters on positively polarized particles dispersed in engine oil: spherical glass beads, fused aluminum oxide powder, and silicon metal powder, all smaller than the mesh opening. The results obtained are used to consider the potential of using AC dielectrophoretic filtration and provide critical design guidelines for the development of a filter based on the retention capability of challenge particles.

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“…This produces large electric field gradients (∇E ~ 10 13 V/m 2 ), and thus large DEP forces (F DEP ∝ E∇E). Our lab has previously demonstrated micro-scale separation of bacteria and polymer microspheres using DEP and an accompanying field-induced phase separation [9,10]. Some of the disadvantages of this technique are the dependence of the polarization effect on the buffer (low ionic strength solutions are needed for trapping DNA) and the unknown effects that the strong electric field will have on the integrity of the DNA molecule.…”

Section: Dielectrophoresis Of Dna Moleculesmentioning

confidence: 99%

“…In conjunction with our colleagues from the New Jersey Institute of Technology, City College of New York, we have developed and demonstrated a new concept, termed "dielectrophoretic gating," a technique that combines the field-induced dielectrophoresis and phase transition for manipulating particles in micro-fluidics [9,10]. Figure 1 depicts the gate, which consists of a pair of microelectrodes that span a fluid channel perpendicular to the direction of fluid flow.…”

Section: Dep Gate Devicementioning

confidence: 99%

Binary electrokinetic separation of target DNA from background DNA primers.

James¹,

Derzon²

2005

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This report contains the summary of LDRD project 91312, titled "Binary Electrokinetic Separation of Target DNA from Background DNA Primers". This work is the first product of a collaboration with Columbia University and the Northeast BioDefense Center of Excellence. In conjunction with Ian Lipkin's lab, we are developing a technique to reduce false positive events, due to the detection of unhybridized reporter molecules, in a sensitive and multiplexed detection scheme for nucleic acids developed by the Lipkin lab. This is the most significant problem in the operation of their capability. As they are developing the tools for rapidly detecting the entire panel of hemorrhagic fevers this technology will immediately serve an important national need.The goal of this work was to attempt to separate nucleic acid from a preprocessed sample. We demonstrated the preconcentration of kilobase-pair length double-stranded DNA targets, and observed little preconcentration of 60 base-pair length single-stranded DNA probes. These objectives were accomplished in microdevice formats that are compatible with larger detection systems for sample pre-processing.Combined with Columbia's expertise, this technology would enable a unique, fast, and potentially compact method for detecting/identifying genetically-modified organisms and multiplexed rapid nucleic acid identification. Another competing approach is the DARPA funded IRIS Pharmaceutical TIGER platform which requires many hours for operation, and an 800k$ piece of equipment that fills a room. The Columbia/SNL system could provide a result in 30 minutes, at the cost of a few thousand dollars for the platform, and would be the size of a shoebox or smaller.4

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Combined field-induced dielectrophoresis and phase separation for manipulating particles in microfluidics

Cited by 39 publications

References 13 publications

An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting

An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting

A novel concept of dielectrophoretic engine oil filter

Binary electrokinetic separation of target DNA from background DNA primers.

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