Combined exposure to UV and PM affect skin oxinflammatory responses and it is prevented by antioxidant mix topical application: Evidences from clinical study

Abstract

BackgroundExposure to environmental stressors like particulate matter (PM) and ultraviolet radiation (UV) induces cutaneous oxidative stress and inflammation and leads to skin barrier dysfunction and premature aging. Metals like iron or copper are abundant in PM and are known to contribute to reactive oxygen species (ROS) production.AimsAlthough it has been suggested that topical antioxidant may be able to help in preventing and/or reducing outdoor skin damage, limited clinical evidence under real‐life exposure conditions have been reported. The aim of the present study was to evaluate the ability of a topical serum containing 15% ascorbic acid, 0.5% ferulic acid, and 1% tocopherol (CF Mix) to prevent oxinflammatory skin damage and premature aging induced by PM + UV in a human clinical trial.MethodsA 4‐day single‐blinded, clinical study was conducted on the back of 15 females (18–40 years old). During the 4 consecutive days, the back test zones were treated daily with or without the CF Mix, followed by with/without 2 h of PM and 5 min of UV daily exposure.ResultsApplication of the CF Mix prevented PM + UV‐induced skin barrier perturbation (Involucrin and Loricrin), lipid peroxidation (4HNE), inflammatory markers (COX2, NLRP1, and AhR), and MMP9 activation. In addition, CF Mix was able to prevent Type I Collagen loss.ConclusionThis is the first human study confirming the multipollutants cutaneous damage and suggesting the utility of a daily antioxidant topical application to prevent pollution induced skin damage.

Gut-derived wild blueberry phenolic acid metabolites modulate extrinsic cutaneous damage

“…Images were then quantified using ImageJ software 1.53a (Java 1.8.0_172, National Institutes of Health, Bethesda, MD, USA). 38 Medium was removed from 24-well plates containing HUVEC cells and rinsed with PBS briefly and fixed with 10% formalin at room temperature for 10 minutes. Cells were then washed three times with PBS for 5 minutes on an automated shaker 30 rpm and then permeabilized with a 0.2% triton x-100 at room temperature.…”