Combined estimation of tetanus and diphtheria antitoxin in human sera by the in vitro Toxin-Binding Inhibition (ToBI) test

Hendriksen, Coenraad; Gun, J.W. van der; Kreeftenberg, J.G.

doi:10.1016/0092-1157(89)90009-7

Cited by 43 publications

(24 citation statements)

References 14 publications

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“…There was no difference in IgG1 concentrations, age distribution, sex ratio, atopic constitution, degree of obstructive lung disease (scored as percent forced expiratory volume in 1 s (FEV 1 ) of predicted), incidence of pneumonia, smoking habits and corticosteroid or antibiotic use between the two groups. In order to define better pneumococcal polysaccharide vaccine responsiveness and non-responsiveness, pre-and post-immunization sera were subsequently analysed for IgG1, IgG2 and IgA (27) antibodies against capsular polysaccharides of seven different pneumococcal serotypes (Fig. 3).…”

Section: Resultsmentioning

confidence: 99%

“…To analyse further the immune response in patients with decreased serum IgG1 levels [26], patients with DTP antibody titres below protective levels were vaccinated (DTP; National Institute of Public Health and the Environment, Bilthoven, The Netherlands) and antibody levels were determined 3 weeks later [27].…”

Section: Anti-capsular Polysaccharide Antibody Assaysmentioning

confidence: 99%

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Clinical and immunological evaluation of patients with mild IgG1 deficiency

Kessel

Horikx

Houte

et al. 1999

Clinical and Experimental Immunology

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SUMMARYSerum IgG subclass concentrations were determined in patients visiting, the pulmonology out-patient clinic with chronic respiratory tract problems. A total of 24 patients with a serum IgG1 concentration < 4·9 g/l (i.e. below the reference range) and normal values for IgG2, IgM and IgA were included. Patients with a selective IgG1 deficiency were vaccinated with a 23-valent pneumococcal polysaccharide vaccine. There were nine patients with a poor antibody response to pneumococcal capsular polysaccharide antigens. Responsiveness to protein antigens was intact in all patients. Patients with pneumonia showed a significantly lower anti-polysaccharide response in the IgG2 subclass than patients without pneumonia. Patients with recurrent sinusitis showed a significantly lower response in the IgA isotype after vaccination with pneumococcal polysaccharide vaccine compared with non-sinusitis patients. It can be concluded that patients with recurrent sinopulmonary infections and a mild IgG1 subclass deficiency have an impaired IgG1 anti-polysaccharide response, which can extend to decreased IgG2 and IgA anti-polysaccharide responses.

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Section: Resultsmentioning

confidence: 99%

Section: Anti-capsular Polysaccharide Antibody Assaysmentioning

confidence: 99%

Clinical and immunological evaluation of patients with mild IgG1 deficiency

Kessel

Horikx

Houte

et al. 1999

Clinical and Experimental Immunology

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“…A range of 2 SD above to 2 SD below the mean for age was considered normal (11,12). Diphtheria and tetanus toxoid antibodies were determined as previously described (13). A titer of less than 0.1 UImL for a fully immunized child was considered below normal (13).…”

Section: Patientsmentioning

confidence: 99%

“…Diphtheria and tetanus toxoid antibodies were determined as previously described (13). A titer of less than 0.1 UImL for a fully immunized child was considered below normal (13). Anti-blood group isohemagglutinins anti-A and anti-B were determined by standard methods.…”

Section: Patientsmentioning

confidence: 99%

Immunoglobulin Isotype-Specific Antibody Responses to Pneumococcal Polysaccharide Vaccine in Patients with Recurrent Bacterial Respiratory Tract Infections

et al. 1995

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“…However, because this assay is also time-consuming and requires cell cul-ture facilities, diagnostic laboratories prefer to use simpleformat indirect enzyme-linked immunosorbent assays (ELISAs), which offer significant advantages in terms of cost, speed, ease of use, and adaptability to automation. Other in vitro methods are available, such as the double-antigen ELISA (DAE) (16), the dual double-antigen, time-resolved fluorescence immunoassay (dDA-DELFIA) (1,4), the passive hemagglutination assay (PHA) (27), the toxin binding inhibition assay (ToBI) (12), and the fluorescent bead-based multiplex assay (24), but none of these are as easy to perform as an indirect ELISA.…”

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confidence: 99%

External Quality Assessment for the Determination of Diphtheria Antitoxin in Human Serum

Giovine

Pinto

Ölander

et al. 2010

Clin Vaccine Immunol

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Accurate determination of diphtheria toxin antibodies is of value in determining the rates of immunity within broad populations or the immune status of individuals who may be at risk of infection, by assessing responses to vaccination and immunization schedule efficacy. Here we report the results of an external quality assessment (EQA) study for diphtheria serology, performed within the dedicated surveillance network DIPNET. Twelve national laboratories from 11 European countries participated by testing a standard panel of 150 sera using their current routine method: Vero cell neutralization test (NT), double-antigen enzyme-linked immunosorbent assay (ELISA; DAE), dual double-antigen time-resolved fluorescence immunoassay (dDA-DELFIA), passive hemagglutination assay (PHA), toxin binding inhibition assay (ToBI), and in-house or commercial ELISAs. The objective of the study was not to identify the best assay, as the advantages and drawbacks of methods used were known, but to verify if laboratories using their routine method would have categorized (as negative, equivocal, or positive) a serum sample in the same way. The performance of each laboratory was determined by comparing its results on a quantitative and qualitative basis to NT results from a single reference laboratory, as this test is considered the in vitro "gold standard." The performance of laboratories using NT was generally very good, while the laboratories' performance using other in vitro methods was variable. Laboratories using ELISA and PHA performed less well than those using DAE, dDA-DELFIA, or ToBI. EQA is important for both laboratories that use in vitro nonstandardized methods and those that use commercial ELISA kits.

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Combined estimation of tetanus and diphtheria antitoxin in human sera by the in vitro Toxin-Binding Inhibition (ToBI) test

Cited by 43 publications

References 14 publications

Clinical and immunological evaluation of patients with mild IgG1 deficiency

Clinical and immunological evaluation of patients with mild IgG1 deficiency

Immunoglobulin Isotype-Specific Antibody Responses to Pneumococcal Polysaccharide Vaccine in Patients with Recurrent Bacterial Respiratory Tract Infections

External Quality Assessment for the Determination of Diphtheria Antitoxin in Human Serum

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