Combined effects of physicochemical variables (pH and salinity) on sperm motility: characterization of sperm motility in European sea bass<i>Dicentrarchus labrax</i>

Öğretmen, Fatih; İnanan, Burak Evren; Kutluyer, Filiz

doi:10.1080/10236244.2016.1163838

Cited by 13 publications

(13 citation statements)

References 27 publications

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“…However, Ingermann et al () reported that sperm motility parameters in Acipenser transmontanus were stimulated at high pH (more than 8.2), but were inhibited at low pH (lower than 7.5). Furthermore, Öğretmen et al () reported that the best sperm motility parameters for European sea bass, Dicentrarchus labrax , were obtained at a pH of 9.0. Conversely, the sperm motility parameters in waigieu seaperch were activated at pH from 6.0 to 9.0, but the optimal sperm motility parameters were observed at pH 8.0.…”

Section: Discussionmentioning

confidence: 99%

Sperm Motilities in Waigieu Seaperch, Psammoperca waigiensis: Effects of Various Dilutions, pH, Temperature, Osmolality, and Cations

Pham

2016

J World Aquaculture Soc

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The objectives of this study were to evaluate the effects of varying dilutions, pH, temperature, osmolality, and cations on sperm motility parameters in waigieu seaperch, Psammoperca waigiensis. The maximum velocity of average in path (VAP), percentage of motile cells (MOT), and duration of sperm motility (DSM) were observed when semen was diluted in artificial seawater (ASW) at a ratio of 1:100 (144.9 ± 0.6 µm/sec, 95.6 ± 0.4%, and 230.3 ± 2.3 sec, respectively), at 30 C (142.0 ± 0.6 µm/sec, 93.6 ± 0.4%, and 238.3 ± 0.9 sec, respectively), and pH 8 (144.8 ± 0.6 µm/sec, 93.3 ± 0.4%, and 234.0 ± 1.5 sec, respectively). Maximum VAP, MOT, and DSM were obtained in each solution containing 0.6 M NaCl (143.8 ± 1.0 µm/sec, 91.3 ± 2.0%, and 230.6 ± 4.2 sec, respectively), 0.6 M KCl (135.1 ± 3.1 µm/sec, 91.1 ± 3.1%, and 230.3 ± 3.7sec, respectively), 0.2M CaCl2 (105.3 ± 4.7μm/sec, 47.9 ± 2.7%, and 120.7 ± 1.3 sec, respectively), 0.2 M MgCl2 (107.3 ± 3.0 m/s, 42.1 ± 3.3%, and 120.3 ± 4.8 sec, respectively), and osmolality of 400 mOsm/kg (145.1 ± 2.5 µm/sec, 93.0 ± 2.1%, and 346.5 ± 4.4s, respectively). We used these mediums as artificial insemination media for fertilizing matured eggs. The results showed that the fertilization and hatching rates in 0.6 M NaCl (75.3 ± 0.6% and 57.0 ± 2.4%, respectively), ASW (70.8 ± 1.2% and 51.2 ± 1.8%, respectively), or 400 mOsm/kg (72.9 ± 1.8% and 55.3 ± 1.6%, respectively) were higher than that in seawater (63.9 ± 1.2% and 39.2 ± 3.9%, respectively). In conclusion, using 0.6 M NaCl, ASW, or 400 mOsm/kg as an artificial insemination medium is effective for fertilizing of waigieu seaperch.

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Section: Discussionmentioning

confidence: 99%

Sperm Motilities in Waigieu Seaperch, Psammoperca waigiensis: Effects of Various Dilutions, pH, Temperature, Osmolality, and Cations

Pham

2016

J World Aquaculture Soc

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“…The percentage of sperm motility was estimated as the cell performing progressive forward movement while the duration of motility was determined as the time until forward movement stops. Determining the percentage of sperm motility was assessed using an arbitrary scale with 10% interval increments in which non motile represents 0% [14,[37][38][39][40].…”

Section: Methodsmentioning

confidence: 99%

“…In particular, populations of the species are affected by natural hybridization, the local devastation in water sources through habitat fragmentation and modifi cation, water eutrophication and contaminations, environmental instability and global warming [9][10][11][12]. Sperm motility is the essential functional parameter for successful fertilization in fi sh [13,14]. Sperm cells in most fi sh species immotile in seminal fl uid and require to release into the water in order to trigger motility and become metabolically active [14,15].…”

Section: Introductionmentioning

confidence: 99%

“…Sperm motility is the essential functional parameter for successful fertilization in fi sh [13,14]. Sperm cells in most fi sh species immotile in seminal fl uid and require to release into the water in order to trigger motility and become metabolically active [14,15]. Therefore, characteristics of activation solutions are crucial in terms of initiation and progression of sperm motility [14].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Effect of Zinc: Evaluation of the Sperm Quality of Endangered Trout Salmo Coruhensis and Rainbow Trout Oncorhynchus Mykiss and Fertilizing Capacity

Kutluyer¹

2017

Int J Aquac Fish Sci

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This study was intended to reveal the usefulness of Zinc in endangered trout Salmo coruhensis and rainbow trout Oncorhynchus mykiss sperm. Spermatozoa were activated in sperm motility-activation solutions (NaCl, 0.3%; NaHCO 3 , 1%) containing the Zinc [Control (0), 0.5, 1, 2, 3, 4 and 5 mM]. The percentage and duration of motility, fertility and hatching rate were determined in sperm samples. Our results indicated that the percentage and duration of motility, fertility and hatching rate increased when activation solution containing NaHCO 3 was supplemented with 1 mM Zinc in rainbow trout (O. mykiss). On the contrary, motility rate in endangered trout (S. coruhensis) was increased by Zinc compared to control group. The percentage and duration of motility, fertility and hatching rate were affected by increasing concentrations of Zinc in endangered trout (S. coruhensis) and rainbow trout (O. mykiss) (p<0.05). In conclusion, sperm quality was affected by quantitative changes different concentrations of Zinc and the best results were obtained from a concentration of 1 mM for rainbow trout.

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“…17,18 Especially in Salmonids sperm cells are immotile in seminal fluid and require to be released into the water in order to become metabolically active. 18,19 As such Salmonid gametes come into direct contact with contaminants 20 and negatively affected. Besides sperm quality, physiological traits are important for monitoring animal health.…”

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confidence: 99%

Cypermethrin-Induced in vitro Alterations on Oxidative Stress and Quality of Salmo coruhensis Spermatozoa

Kutluyer¹,

Kocabaş²,

Erişir³

et al. 2018

IJPER

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The use of insecticides has been increasing along with increasing agriculture activities and has caused deleterious environmental impacts. Non-target organisms in particular, including fish, are affected by pesticides. In this work, the impacts of cypermethrin (CYP) on sperm oxidative stress markers and sperm motility were investigated in vitro. The CYP concentrations were 0 μg L -1 control, ethanol), 1.025 μg L (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT)] activities in sperm cells were examined for determination of oxidative stress status. Our findings showed that motility and survival of sperm cells significantly decreased with exposure to CYP. Biochemical assays revealed that CAT activity and levels of MDA and, GSH increased in spermatozoa based on CYP concentration while activity of GSH-Px and SOD decreased. Consequently, spermatozoa were highly sensitive to CYP exposure. It can be deduced that CYP has the potential to disrupt sperm quality and to cause to oxidative stress in sperm cells of S. coruhensis.

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Combined effects of physicochemical variables (pH and salinity) on sperm motility: characterization of sperm motility in European sea bassDicentrarchus labrax

Cited by 13 publications

References 27 publications

Sperm Motilities in Waigieu Seaperch, Psammoperca waigiensis: Effects of Various Dilutions, pH, Temperature, Osmolality, and Cations

Sperm Motilities in Waigieu Seaperch, Psammoperca waigiensis: Effects of Various Dilutions, pH, Temperature, Osmolality, and Cations

In Vitro Effect of Zinc: Evaluation of the Sperm Quality of Endangered Trout Salmo Coruhensis and Rainbow Trout Oncorhynchus Mykiss and Fertilizing Capacity

Cypermethrin-Induced in vitro Alterations on Oxidative Stress and Quality of Salmo coruhensis Spermatozoa

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