Combined effects of magnetic fields and temperature changes on 1-aminonaphthalene-8-sulfonic acid fluorescence in red blood cell ghost cell membrane

Abstract

In the present study, we performed an experiment to clarify the possible effects of magnetic fields of up to 8 T on cell membrane fluidity by using red blood cell ghosts and a fluorescence dye, 1-aminonaphthalene-8-sulfonic acid (ANS). The time course of ANS emission at 480 nm under the influence of a magnetic field at 5 T was observed. The effects of multiple rapid temperature changes and magnetic fields were investigated. The emission intensity at 480 nm increased when the temperature of the cell holder was increased from 20 to 38–46 °C for 15 min. A change in temperature exhibited an increase in the fluidity of the lipid molecules in the cell membrane and increased the population of ANS molecules emitting light at 480 nm in the cell membrane, which is hydrophobic. A discontinuous change in fluorescence at 38–40 °C was exhibited under exposure to a magnetic field at 5 T, while the temperature dependency was continuous without exposure to the magnetic field. In addition, under exposure to the magnetic field, the fluorescence during a decrease in temperature from 38 to 20 °C remained at a level close to the fluorescence during an increase in temperature. The results indicated that the fluidity of the molecules in the cell membrane was decelerated by exposure to magnetic fields at 5 T. We speculated that the magnetic orientation in a part of the lipid membrane disturbed the release of ANS molecules from a hydrophobic region of the membrane.

Microbicidal effects of magnetic field and irradiation on plant pathogenic Thielaviopsis paradoxa and Pectobacterium cartovorum

“…In a safety cabinet (laminar flow), a UV lamp is used with wavelength of 100-280 nm and the distance from UV tip to microorganism were set to 20 cm, and the current UV was set to 0.35 A to irradiate LED light (Liu et al, 2019). The magnetic field strength of 10 Gauss was measured by Gauss meter, and the experimental cultured groups were placed with the magnetic field beside them (Liu et al, 2022).…”

“…Paradoxa on solid medium and how it was influenced by the magnetic field that the growth diameter was reduced on both liquid and solid media. High-intensity magnetic fields may be can influence cell membrane fluidity and other properties (Iwasakaa et al, 2006) Figure ( 4) shows magnetic fields' effect on the bacterial growth of Pe.cartovorum in both nutrient broth (liquid medium) and nutrient agar (solid medium) at comparable nutrient concentrations. Generally, the magnetic fieldexposed samples grow at a slower rate.…”