Combined effects of klotho and soluble CD40 ligand on A549 lung cancer cells

Wang, Xueli; Chen, Bin; Xu, Wei; Liu, Shifeng; Zhao, Weihong; Wu, Jianzhong

doi:10.3892/or.2011.1178

Cited by 7 publications

(4 citation statements)

References 22 publications

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“… 13 However, reported functions of CD40 in tumor cells vary, with both pro-apoptotic and anti-proliferative effects observed depending on the cellular context. 14 , 15 , 16 Alternatively, some studies have shown that CD40 activation may promote the neoplastic transformation and growth of normal cells. 17 , 18 , 19 Expression of CD40 has been demonstrated in ovarian cancer cell lines and tumor samples, but not in normal ovarian tissue, suggesting that CD40 may have an important role in ovarian tumors.…”

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confidence: 99%

CD40 ligand induces RIP1-dependent, necroptosis-like cell death in low-grade serous but not serous borderline ovarian tumor cells

et al. 2015

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Ovarian high-grade serous carcinomas (HGSCs) and invasive low-grade serous carcinomas (LGSCs) are considered to be distinct entities. In particular, LGSCs are thought to arise from non-invasive serous borderline ovarian tumors (SBOTs) and show poor responsiveness to conventional chemotherapy. The pro-apoptotic effects of CD40 ligand (CD40L) have been demonstrated in HGSC, though the underlying mechanisms are not fully understood. Conversely, the therapeutic potential of the CD40L-CD40 system has yet to be evaluated in LGSC. We now show that CD40 protein is focally expressed on tumor cells in two of five primary LGSCs compared with no expression in eight primary SBOTs. Treatment with CD40L or agonistic CD40 antibody decreased the viability of LGSC-derived MPSC1 and VOA1312 cells, but not SBOT3.1 cells. Small interfering RNA (siRNA) targeting CD40 was used to show that it is required for these reductions in cell viability. CD40L treatment increased cleaved caspase-3 levels in MPSC1 cells though, surprisingly, neither pan-caspase inhibitor nor caspase-3 siRNA reversed or even attenuated CD40L-induced cell death. In addition, CD40-induced cell death was not affected by knockdown of the mitochondrial proteins apoptosis-inducing factor (AIF) and endonuclease G (EndoG). Interestingly, CD40L-induced cell death was blocked by necrostatin-1, an inhibitor of receptor-interacting protein 1 (RIP1), and attenuated by inhibitors of RIP3 (GSK'872) or MLKL (mixed lineage kinase domain-like; necrosulfonamide). Our results indicate that the upregulation of CD40 may be relatively common in LGSC and that CD40 activation induces RIP1-dependent, necroptosis-like cell death in LGSC cells.

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confidence: 99%

CD40 ligand induces RIP1-dependent, necroptosis-like cell death in low-grade serous but not serous borderline ovarian tumor cells

et al. 2015

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“…Third, besides miR‐206, several other miRNAs, such as miR‐516b, miR‐550, and miR‐671‐5p, are also involved in prolactinoma development. [ 19 ] Thus, future studies need to investigate whether curcumin exerts similar regulatory effects on other miRNAs.…”

Section: Discussionmentioning

confidence: 99%

“…[ 16,17 ] MiR‐206 is a proapoptotic activator that is crucial for apoptosis regulation. [ 18 ] An miRNA microarray‐based clinical study [ 19 ] showed distinctly higher expression levels of miR‐206 in the tumor tissues of patients with prolactinoma treated with bromocriptine compared with the expression levels of miR‐206 before treatment. Additionally, miR‐206 levels were positively correlated with treatment outcomes, suggesting that it inhibits prolactinoma development, but the specific signaling pathway involved remains unelucidated.…”

Section: Introductionmentioning

confidence: 99%

Curcumin affects autophagy of prolactinoma cells by upregulating miR‐206 to exert antitumor effects

Duan,

Zhang,

Zhu

et al. 2024

J Biochem & Molecular Tox

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We explored the effects of curcumin on the aberrant biological behaviors of prolactinoma cells and the downstream pathways through which curcumin exerts its antitumor effects. We used quantitative reverse transcription‐polymerase chain reaction assays to measure miR‐206 expression levels in peripheral blood samples from patients with prolactinoma before and after curcumin treatment. We also investigated the proliferation level, viability, and invasion ability of groups of cells treated with different concentrations of curcumin using 3‐(4,5)‐dimethylthiahiazo (‐z‐y1)‐3‐di‐phenytetrazoliumromide (MTT) assays, cell cloning assays, and Transwell assays, respectively. Furthermore, we determined the levels of autophagy‐related proteins and protein kinase B/mammalian target of the rapamycin (Akt/mTOR) signaling pathway‐related proteins in each group of treated cells by western blot. Curcumin treatment upregulated miR‐206 expression levels in the peripheral blood of patients with prolactinoma and in GH3 cells. Knockdown of miR‐206 expression enhanced the proliferation and invasive ability of GH3 cells, while curcumin treatment effectively inhibited the aberrant biological behavior of GH3 cells enhanced by miR‐206 knockdown. miR‐206 knockdown also activated the Akt/mTOR signaling pathway and inhibited autophagy in GH3 cells, and these changes were effectively reversed by curcumin treatment. Thus, curcumin inhibited the Akt/mTOR signaling pathway and promoted cell autophagy by miR‐206 upregulation, resulting in antitumor effects that inhibited prolactinoma cell proliferation and invasion.

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“…The cytotoxicity and targeted delivery of dual drug-loaded nanocomposites were investigated by using MCF-7 and A549 cells. The human lung carcinoma epithelial cells of A549 have high expression levels of CD40 receptor, 66 while the human breast cancer cells of MCF-7 have negligible CD40 expressions. 67 The cell surface receptor of CD40 was demonstrated to be the prognostic biomarker and therapeutic target for tumor treatment that can be recognized by anti-CD40 antibodies as the "target" for highly efficient drug endocytosis.…”

Section: Mri Characterization Ofmentioning

confidence: 99%

Multi-Armed Anti-CD40-Mediated Dual Drug Delivery System Based on Mesoporous Silica/Au Nanorod Nanocomposites for Multimodality Imaging and Combination Therapy

Feng

Zhao

et al. 2023

ACS Appl. Nano Mater.

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Nanotechnology provides the platform to develop functional nanocomposites with outstanding features, such as persistent luminescence (PL), photothermal conversion, magnetic resonance (MR), and so on, that has been incorporated in nanomedicine for diagnosis and therapy. Herein, the nanocomposites of ZnGa 2 O 4 :Cr 3+ embedded in mesoporous silica coating surrounding gold nanorods (cGNR@MSNs) were developed for the codelivery of chemotherapeutic drugs and anti-CD40 antibodies. The nanocomposites were functionalized with tetrazaheterocycle linking divinylpyrimidine derivatives (DOTVs), which covalently capture anti-CD40 by disulfide re-bridging on the surface of cGNR@MSNs. The anti-CD40-mediated dual drug delivery system simultaneously realized the combination of chemo-, immuno-, and photothermal therapies, enhancing the synergistic effect of combination therapy for multiple tumor cells. Moreover, the nanodrug delivery system achieved multimodality imaging with fluorescence, PL, and MR modes for precise diagnosis. This study provides fascinating insights into the development of nanocomposite-based multimodality imaging strategies and imagingguided combination therapies.

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Combined effects of klotho and soluble CD40 ligand on A549 lung cancer cells

Cited by 7 publications

References 22 publications

CD40 ligand induces RIP1-dependent, necroptosis-like cell death in low-grade serous but not serous borderline ovarian tumor cells

CD40 ligand induces RIP1-dependent, necroptosis-like cell death in low-grade serous but not serous borderline ovarian tumor cells

Curcumin affects autophagy of prolactinoma cells by upregulating miR‐206 to exert antitumor effects

Multi-Armed Anti-CD40-Mediated Dual Drug Delivery System Based on Mesoporous Silica/Au Nanorod Nanocomposites for Multimodality Imaging and Combination Therapy

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