Combined effects of high stocking density and Piscirickettsia salmonis treatment on the immune system, metabolism and osmoregulatory responses of the Sub-Antarctic Notothenioid fish Eleginops maclovinus

Vargas‐Chacoff, Luis; Martínez, D.; Oyarzún, R.; Nualart, Daniela; Olavarría, Víctor H.; Yáñez, Alejandro J.; Bertrán, Carlos; Ruíz-Jarabo, Ignacio; Mancera, Juan Miguel

doi:10.1016/j.fsi.2014.07.024

Cited by 47 publications

(27 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plasma proteins were determined using the commercial kit, BCA Protein Assay ® , adapted to microplates (#23225). Total α‐amino acid levels were assessed colorimetrically using the Ninhydrin method (Moore, ; Vargas‐Chacoff, Martinez, et al, ). All assays were performed with the Microplate Reader Multiscango (Thermo Scientific), using the software Scan 3.2 to Multiscango (320 nm glucose, 505 nm lactate, 540 nm triglycerides and proteins).…”

Section: Methodsmentioning

confidence: 99%

“…Cortisol levels were measured by ELISA using a commercial kit from DIA source Immuno Assay S.A. (Cortisol Ref. KAPDB270) as previously outlined by Vargas‐Chacoff, Martinez, et al ().…”

Section: Methodsmentioning

confidence: 99%

“…The latter, E. maclovinus , is a species of great economic importance in the artisanal and recreational fisheries, particularly in marine and estuarine areas where large numbers of these fish congregate near salmon sea cages. This Sub‐Antarctic notothenioid fish is distributed in Chile between Valparaiso and Tierra del Fuego (Pequeño, ), and their biological characteristics (eurythermic, euryhaline and stenobathic) along with their ease of transition to culture conditions make it an ideal model of study in laboratory conditions (Martínez, Vargas‐Lagos, et al, ; Vargas‐Chacoff, Martinez, et al, ). Importantly, E. maclovinus is susceptible to P. salmonis infections while interacting with the infected cultured salmon as they feed on pelleted food dispensed at the sea cages (Martínez, Díaz‐Ibarrola, et al, ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Intermediary metabolic response and gene transcription modulation on the Sub‐Antarctic notothenioid Eleginops maclovinus (Valenciennes, 1930) injected with two strains of Piscirickettsia salmonis

Soto‐Dávila

Martínez

Oyarzún

et al. 2019

Journal of Fish Diseases

View full text Add to dashboard Cite

Pathogen interactions with cultured fish populations are well studied, but their effects on native fishes have not been characterized. In Chile, the disease caused by bacterial species Piscirickettsia salmonis represents one of the main issues and is considered to be one of the important pathogens in the field of aquaculture. They have been found to infect native fish. Therefore, it is necessary to understand the impact of P. salmonis on native species of local commercial value, as well as the potential impact associated with the emergence of antibiotic‐resistant strains of P. salmonis. Due to this purpose, the native fish Eleginops maclovinus was used in our study. Fish were randomly distributed in tanks and intraperitoneally inoculated with two strains of P. salmonis. No mortality was recorded during the experiment. Cortisol, glucose and total α‐amino acid levels increased in fish injected with AUSTRAL‐005 strain compared to sham‐injected and LF‐89‐inoculated fish. Moreover, results showed an increase in the activity of carbohydrates and lipids metabolism in liver; and an increase in the carbohydrates, lipids and total α‐amino acid metabolism in muscle after injection with AUSTRAL‐005. Our results suggest that P. salmonis modulates the physiology of E. maclovinus and the physiological impact increase in the presence of the antibiotic‐resistant strain AUSTRAL‐005.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Cortisol levels were measured by ELISA using a commercial kit from DIA source Immuno Assay S.A. (Cortisol Ref. KAPDB270) as previously outlined by Vargas‐Chacoff, Martinez, et al ().…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Intermediary metabolic response and gene transcription modulation on the Sub‐Antarctic notothenioid Eleginops maclovinus (Valenciennes, 1930) injected with two strains of Piscirickettsia salmonis

Soto‐Dávila

Martínez

Oyarzún

et al. 2019

Journal of Fish Diseases

View full text Add to dashboard Cite

show abstract

“…Animal welfare is particularly applicable to the aquaculture industry given the existing relationship between stressful situations and poor immune system responses in cultivated fish, which provokes increased rates of pathogenic infection (Dhabhar 2009, Tort 2011, Vargas-Chacoff et al 2014. There are a wide variety of stressful stimuli in salmonid farming, among which, animal management and confinement are the most significant (Kestin 1994).…”

Section: Introductionmentioning

confidence: 99%

“…The first is an acute response to short-term events, such as fish capture and handling, biometric analyses, and transport. The second is a chronic response to persistent, long-term conditions such as high stock density, variations in water quality, exposure to new environments, social dominance, and exposure to certain diseases (Wendelaar Bonga 1997, Polakoff et al 2006, Tort 2011, Vargas-Chacoff et al 2014. Salt baths are one of the management procedures used by the aquaculture industry.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical parameters associated with the methds of application of salt baths and their field assessment of blood parameters of Atlantic salmon in water pre-smolt stage

et al. 2016

View full text Add to dashboard Cite

ABSTRACT. Salt baths in the production system of salmonids in fresh water, are important for the prevention and control of different diseases such as saprolegnia fungus. This standard procedure generates a rapid depletion of O 2 in the water, leading to a significant increase of CO 2 , affecting blood parameters associated with animal welfare, such as Na + , Cl -, glucose and protracted hypercarbia. The aim of this study was to evaluate the response field of blood parameters related to fish welfare and the physical-chemical parameters of water to which they are subject during this period, comparing two sets of bath salt. One group of farmed Atlantic salmon (Salmo salar) in the pre-smolt stage was exposed in a salt bath system with the usual conditions and a second to a recirculation system with a waterfall for degasification, with two different exposure periods of one and two hours. The results of the concentration of CO 2 in the water show significant variations between the two systems without recirculation compared to the control condition and salt. Significant differences were observed between the recirculating system and the control in blood levels of pCO 2 and HCO 3 , but not pH. Our field data also show that salt baths that are performed regularly at the fish farms generate significant effects on blood parameters associated with animal welfare specifically fish pCO 2 and HCO 3 causing minor hypercarbia without generating blood acidosis. The addition of water recirculation with a waterfall does not affect blood parameters.Key words: salt bath, water chemistry, saprolegnia, animal welfare. RESUMEN.Los baños de sal, dentro del sistema productivo de salmónidos en agua dulce, son un paso clave en la prevención y el control de diferentes enfermedades como la saprolegnia. Este procedimiento habitual genera un agotamiento rápido del O 2 en el agua, lo que genera un aumento significativo del CO 2 en el agua, afectando parámetros sanguíneos asociados a bienestar animal, como Na + , Cl -, glucosa y en situaciones prolongadas hipercapnia. Este trabajo tiene como objetivo evaluar en terreno la respuesta de parámetros sanguíneos asociados a bienestar de los peces y parámetros físico-químicos del agua a la que están sometidos en este periodo, comparando dos sistemas de baño de sal. Para ello se sometió a un grupo de Salmo salar en etapa pre-smolt a un baño de sal habitual, otro grupo a un sistema de recirculación con cascada de agua para su desgasificación y un grupo control sin sal, exponiéndolos a diferentes periodos de una y dos horas. Los resultados de la concentración de CO 2 en el agua muestran variaciones significativas entre ambos sistemas con y sin recirculación para la condición control y con sal. Los niveles sanguíneos evaluados en terreno de PCO 2 y HCO 3 presentaron diferencias significativas respecto del sistema con recirculación y al control, no así los niveles sanguíneos de pH. Nuestros datos de terreno demuestran que los baños de sal que se realizan con regularidad en los centros de cultivo generan efect...

show abstract

Effects of acclimation to high environmental temperatures on intermediary metabolism and osmoregulation in the sub-Antarctic notothenioid Eleginops maclovinus

et al. 2017

View full text Add to dashboard Cite

Combined effects of high stocking density and Piscirickettsia salmonis treatment on the immune system, metabolism and osmoregulatory responses of the Sub-Antarctic Notothenioid fish Eleginops maclovinus

Cited by 47 publications

References 49 publications

Intermediary metabolic response and gene transcription modulation on the Sub‐Antarctic notothenioid Eleginops maclovinus (Valenciennes, 1930) injected with two strains of Piscirickettsia salmonis

Intermediary metabolic response and gene transcription modulation on the Sub‐Antarctic notothenioid Eleginops maclovinus (Valenciennes, 1930) injected with two strains of Piscirickettsia salmonis

Physicochemical parameters associated with the methds of application of salt baths and their field assessment of blood parameters of Atlantic salmon in water pre-smolt stage

Effects of acclimation to high environmental temperatures on intermediary metabolism and osmoregulation in the sub-Antarctic notothenioid Eleginops maclovinus

Contact Info

Product

Resources

About