Combined effects of high-fat diet and polystyrene microplastic exposure on microplastic bioaccumulation and lipid metabolism in zebrafish

Du, Jiuyu; Hu, Yanqiu; Hou, Min; Zhou, Jianhong; Zheng, Hao; Zhang, Tingjun; He, Xuelian; Han, Xianpei

doi:10.1016/j.fsi.2023.108803

Cited by 12 publications

(10 citation statements)

References 31 publications

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“…The morphology of the different sizes of PS MPs in the water was observed using a scanning electron microscope (HITACHI, SU8100, Japan), and the detailed experimental procedures were described in the previous study (Du et al, 2023).…”

Section: Chemicalsmentioning

confidence: 99%

“…Zebrafish embryos were obtained from wild-type adult zebrafish, and all zebrafish were maintained according to standard protocols provided by the Chinese Zebrafish Resource Center. Zebrafish maintenance is based on a previous study (Du et al, 2023). In short, zebrafish were cultured in a stable circulating filtration system and were fed twice daily with live brine shrimp.…”

Section: Zebrafish Maintenancementioning

confidence: 99%

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The combined exposure of polystyrene microplastics and high‐fat feeding affects the intestinal pathology damage and microbiome in zebrafish

Huang,

Hu,

Zhang

et al. 2024

Journal of Fish Biology

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The pervasive utilization of plastics and their integration into ecosystems has resulted in significant environmental issues, particularly the pollution of microplastics (MPs). In aquaculture, high‐fat feed (HFD) is frequently employed to enhance the energy intake and economic fish production. This study utilized zebrafish as a model organism to investigate the impact of concurrent exposure to HFD and MPs on fish intestinal pathology damage and intestinal microbiome. The experimental design involved the division of zebrafish into two groups: one receiving a normal diet (ND) and the other receiving HFD. The zebrafish were exposed to a control group, as well as polystyrene (PS) MPs of varying sizes (5 and 50 μm). Histopathological examination revealed that the combination of 5 μm MPs and HFD resulted in the most significant damage to the zebrafish intestinal tract. Furthermore, gut microbiome assays indicated that exposure to MPs and HFD altered the composition of the gut microbiome. This study demonstrates that in aquaculture, the issue of HFD must be considered alongside concerns about MPs contamination, as both factors appear to have a combined effect on the intestinal pathology damage and intestinal microbiome. The findings of this research offer valuable insights for the improvement of fish farming practices.

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Section: Chemicalsmentioning

confidence: 99%

Section: Zebrafish Maintenancementioning

confidence: 99%

The combined exposure of polystyrene microplastics and high‐fat feeding affects the intestinal pathology damage and microbiome in zebrafish

Huang,

Hu,

Zhang

et al. 2024

Journal of Fish Biology

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“…At the same time, because the detection technology of NP concentrations in the environment is not highly developed, only one study has reported on the concentration of NPs in the environment (Allen et al, 2022); however, there are several reports on the concentrations of microplastics in surface water and the environment (Fu & Wang, 2019;Zhu et al, 2018). We pooled the literature and environmental microplastic and NP concentrations to determine the working concentration of NPs by observing the morphology of polystyrene NPs in water using scanning electron microscopy (model SU8100; Hitachi), as described in previous studies (Du et al, 2023).…”

Section: Chemicalsmentioning

confidence: 99%

“…Specific methods have been described in previous studies (Du et al, 2023). In brief, we used the AB strain of zebrafish.…”

Section: Zebrafish Maintenancementioning

confidence: 99%

Toxicity of Polystyrene Nanoplastics in the Liver and Intestine of Normal and High‐Fat‐Diet Juvenile Zebrafish

Du,

Hu,

et al. 2023

Enviro Toxic and Chemistry

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Nanoplastics (NPs) are widely found and threaten environmental and biological safety, because they do not degrade completely. We aimed to preliminarily explore the toxicity of NPs in obese children, as childhood obesity is a growing global health concern. As such, in this study, zebrafish were used as a vertebrate toxicological model to examine the hepatic lipid metabolism and gut microbiota in juvenile zebrafish exposed to 1000 μg/L PS NPs and high‐fat diet (HFD)using Raman spectroscopy, pathological examination, transcriptome, and 16S sequencing techniques. Our study showed that PS NPs perturb the lipid metabolism and gut microbiota stability in zebrafish. Furthermore, the combined effects of PS NPs and HFD resulted in gastrointestinal injury. This study is one of the first to investigate the toxicity of PS NPs to normal diet (ND) and HFD juvenile zebrafish using confocal Raman spectroscopy. Our results signify the importance of a healthy diet and a reduction in the use of plasticware.

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“…Microplastics can enter the human body via consumption and inhalation, so the air and food are the main sources of human microplastic uptake [ 11 ]. Microplastics can be present in fast food, takeout, beverages, and other high-fat diets (HFD) due to contamination during production, processing, packaging, and storage, accelerating the disruption of systemic metabolism [ 3 , 12 , 13 ]. Estimates of microplastic ingestion through HFD range from a few tens to hundreds of particles per day for humans [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Single-cell RNA-seq analysis decodes the kidney microenvironment induced by polystyrene microplastics in mice receiving a high-fat diet

Xu,

Ye,

Liu

et al. 2024

J Nanobiotechnol

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In recent years, the environmental health issue of microplastics has aroused an increasingly significant concern. Some studies suggested that exposure to polystyrene microplastics (PS-MPs) may lead to renal inflammation and oxidative stress in animals. However, little is known about the essential effects of PS-MPs with high-fat diet (HFD) on renal development and microenvironment. In this study, we provided the single-cell transcriptomic landscape of the kidney microenvironment induced by PS-MPs and HFD in mouse models by unbiased single-cell RNA sequencing (scRNA-seq). The kidney injury cell atlases in mice were evaluated after continued PS-MPs exposure, or HFD treated for 35 days. Results showed that PS-MPs plus HFD treatment aggravated the kidney injury and profibrotic microenvironment, reshaping mouse kidney cellular components. First, we found that PS-MPs plus HFD treatment acted on extracellular matrix organization of renal epithelial cells, specifically the proximal and distal convoluted tubule cells, to inhibit renal development and induce ROS-driven carcinogenesis. Second, PS-MPs plus HFD treatment induced activated PI3K-Akt, MAPK, and IL-17 signaling pathways in endothelial cells. Besides, PS-MPs plus HFD treatment markedly increased the proportions of CD8+ effector T cells and proliferating T cells. Notably, mononuclear phagocytes exhibited substantial remodeling and enriched in oxidative phosphorylation and chemical carcinogenesis pathways after PS-MPs plus HFD treatment, typified by alterations tissue-resident M2-like PF4+ macrophages. Multispectral immunofluorescence and immunohistochemistry identified PF4+ macrophages in clear cell renal cell carcinoma (ccRCC) and adjacent normal tissues, indicating that activate PF4+ macrophages might regulate the profibrotic and pro-tumorigenic microenvironment after renal injury. In conclusion, this study first systematically revealed molecular variation of renal cells and immune cells in mice kidney microenvironment induced by PS-MPs and HFD with the scRNA-seq approach, which provided a molecular basis for decoding the effects of PS-MPs on genitourinary injury and understanding their potential profibrotic and carcinogenesis in mammals. Graphical Abstract

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Combined effects of high-fat diet and polystyrene microplastic exposure on microplastic bioaccumulation and lipid metabolism in zebrafish

Cited by 12 publications

References 31 publications

The combined exposure of polystyrene microplastics and high‐fat feeding affects the intestinal pathology damage and microbiome in zebrafish

The combined exposure of polystyrene microplastics and high‐fat feeding affects the intestinal pathology damage and microbiome in zebrafish

Toxicity of Polystyrene Nanoplastics in the Liver and Intestine of Normal and High‐Fat‐Diet Juvenile Zebrafish

Single-cell RNA-seq analysis decodes the kidney microenvironment induced by polystyrene microplastics in mice receiving a high-fat diet

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