Combined effects of acute thermal and hypo-osmotic stresses on osmolality and hsp70, hsp90 and sod expression in the sea cucumber Apostichopus japonicus Selenka

Metabolomic responses of sea cucumber Apostichopus japonicus to thermal stresses

“…Accumulative evidences have shown that HSPs and SOD are expressed in response to a varieties of environmental factors including thermal stresses Ji et al, 2008;Wang et al, 2013Wang et al, , 2014, and are often regarded as promising candidate markers for monitoring environmental changes (Feder and Hofmann, 1999;Parihar et al, 1997). In our work, the significant up-regulation of HSP70 mRNA level and SOD activity at day 0 indicated that host homeostasis was disturbed under the acute thermal stresses, resulting in metabolic alterations accordingly.…”

“…Concomitant with the greenhouse effect, the average global temperature has increased by approximately 0.6°C over the last three decades (IPCC, 2007). The elevated temperature in marine environments has resulted in great ecosystem effect like shift in biogeographic ranges and temperature adaptation for some marine organisms (Abele and Puntarulo, 2004;Ji et al, 2008;Wang et al, 2014). Therefore, understanding of the responsive mechanism of A. japonicus towards thermal stress is necessary for establishing temperature-tolerance strains in its aquaculture.…”

Effect of acute salinity stress on ion homeostasis, Na+/K+-ATPase and histological structure in sea cucumber Apostichopus japonicus

“…However, there are still some questions need to be solved in the sea cucumber breeding industry. Sea cucumber aquaculture ponds located mainly in the intertidal zone, so fluctuation of salinity in its natural habitat frequently occurred due to water exchange, evaporation and precipitation (Dong et al 2008; Wang et al 2014; Bai et al 2015). Sharp salinity changes may also affect the feed intake and higher energy utilization for osmoregulation resulting in poor growth of sea cucumbers, and can even lead to discharge intestine and death (Fankboner 2002).…”

Sources of marine superoxide dismutases: Characteristics and applications

“…Their half-lives were similarly long at 65 • C (<110 min) but exhibited a twofold difference at 80 • C (20.8 vs 9.8 min) [63] Apostichopus japonicus Increase levels of Sod mRNA by thermal and osmotic stresses [64] electrophoresis in reducing conditions; the subunit molecular mass was 18.8 kDa value that is remarkably higher than the one reported for other Cu/Zn-SODs. The isoelectric point, assessed by isoelectric focusing, is 6.35.…”

“…In low salinity environments, the cellular level stress was indicated by SOD mRNA, and the maximal expression of all genes occurred at 6 h after stresses. The up-regulation of SOD mRNA indicated the emergence of protein denaturation and oxidative damage and also suggested an increase in energy consumption at high temperature and low salinity [64].…”