Combined effect of temperature and a reference toxicant (KCl) on Daphnia middendorffiana (Crustacea, Daphniidae) in a high-mountain lake

Pastorino, Paolo; Prearo, Marino; Anselmi, Serena; Bentivoglio, Tecla; Esposito, Giuseppe; Bertoli, Marco; Pizzul, Elisabetta; Barceló, Damià; Elia, Antonia Concetta; Renzi, Monia

doi:10.1016/j.ecolind.2022.109588

Cited by 9 publications

(1 citation statement)

References 48 publications

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“…However, the potential effects of MPs in combination with commonly found water contaminants, such as pesticides, herbicides, heavy metals, and micropollutants, remain unclear (Aliko et al, 2022; Arif et al, 2022; Banaee et al, 2022; Gu et al, 2020; Ravi et al, 2023). Given that MPs are suspected to interact with hydrophobic substances, potentially acting as vectors and leading to increased contaminant accumulation, the potential influence of MPs on waterborne caffeine warrants investigation (Amelia et al, 2021; Burgos‐Aceves et al, 2022; de Sá et al, 2018; Menéndez‐Pedriza & Jaumot, 2020; Pastorino et al, 2023). Previous combined exposures of bivalve mollusks to MPs and various organic substances have demonstrated multistress effects distinct from single exposures (González‐Soto et al, 2019; Martyniuk et al, 2023; Martyniuk, Gylytė, et al, 2022; Martyniuk, Khoma, et al, 2022; Syberg et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

Impellitteri,

Briglia,

Porcino

et al. 2024

Microscopy Res & Technique

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In recent years, the presence of pharmaceuticals and microplastics (MPs) in aquatic ecosystems has raised concerns about their environmental impact. This study explores the combined effects of caffeine, a common pharmaceutical pollutant, and MPs on the marine mussel Mytilus galloprovincialis. Caffeine, at concentrations of 20.0 μg L−1, and MPs (1 mg L−1, 35–50 μm size range), was used to mimic real‐world exposure scenarios. Two hundred M. galloprovincialis specimens were divided into four groups: caffeine, MPs, Mix (caffeine + MPs), and Control. After a two‐week acclimation period, the mollusks were subjected to these pollutants in oxygen‐aerated aquariums under controlled conditions for 14 days. Histopathological assessments were performed to evaluate gill morphology. Cellular volume regulation and digestive gland cell viability were also analyzed. Exposure to caffeine and MPs induced significant morphological changes in M. galloprovincialis gills, including cilia loss, ciliary disk damage, and cellular alterations. The chitinous rod supporting filaments also suffered damage, potentially due to MP interactions, leading to hemocyte infiltration and filament integrity compromise. Hemocytic aggregation suggested an inflammatory response to caffeine. In addition, viability assessments of digestive gland cells revealed potential damage to cell membranes and function, with impaired cell volume regulation, particularly in the Mix group, raising concerns about nutrient metabolism disruption and organ function compromise. These findings underscore the vulnerability of M. galloprovincialis to environmental pollutants and emphasize the need for monitoring and mitigation efforts.Research Highlights The synergy of caffeine and microplastics (MPs) in aquatic ecosystems warrants investigation. MPs and caffeine could affect gill morphology of Mytilus galloprovincialis. Caffeine‐exposed cells had lower viability than the control group in the NR retention test. MPs and mix‐exposed cells struggled to recover their volume.

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