Combined effect of solvent content, temperature and pH on the chromatographic behaviour of ionisable compounds

Relationship between the particle size distribution of commercial fully porous and superficially porous high-performance liquid chromatography column packings and their chromatographic performance

“…This is due to the change of the degree of ionization of the molecule in the hydro-organic environment, with respect to its ionization in pure water, upon the addition of ∼60% of acetonitrile [12,13]. The neutral state of the molecule in the various mobile phases was also confirmed by both UV spectra in aqueous-organic mixtures and by the trend of the retention time recorded at different buffer pH (data gathered from GlaxoSmithKline, not shown here).…”

A chromatographic objective function to characterise chromatograms with unknown compounds or without standards available

“…The new approach is based on the measurement of peak prominences (Section 2.3), and was evaluated by scoring simulated chromatograms in comparison with the peak purity criterion, which in previous work has been demonstrated to offer an excellent performance [3,10,12,[24][25][26]. However, the peak purity is only accessible when the standards for all compounds in a sample are available.…”

Simultaneous determination of celiprolol HCl and chlorthalidone in tablets and biological fluids using high-performance liquid chromatography

“…Various methods have been reported for its (a) Celiprolol HCl (b) Chlorthalidone determination, individually or in combination with other drugs. The most recent chromatographic articles include HPLC [16][17][18][19][20][21][22] and LC-MS [23,24].…”