Combined effect of shear stress and laser-patterned topography on Schwann cell outgrowth: synergistic or antagonistic?

Babaliari, Eleftheria; Kavatzikidou, Paraskevi; Mitraki, Anna; Papaharilaou, Yannis; Ranella, Anthi; Stratakis, Emmanuel

doi:10.1039/d0bm01218a

Cited by 10 publications

(12 citation statements)

References 57 publications

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“…The microenvironment is responsible for the stemness and lineage diversity capacities of the stem cells [ 84 ]. Another factor that drastically impacts characteristics, such as morphology and cell viability, is the shear stress-induced alignment [ 85 ]. Cell activity can be modulated by the microstructure of the utilized material [ 86 ].…”

Section: Mesenchymal Stem Cells Influence In Btementioning

confidence: 99%

Bone Regeneration and Oxidative Stress: An Updated Overview

et al. 2022

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Bone tissue engineering is a complex domain that requires further investigation and benefits from data obtained over past decades. The models are increasing in complexity as they reveal new data from co-culturing and microfluidics applications. The in vitro models now focus on the 3D medium co-culturing of osteoblasts, osteoclasts, and osteocytes utilizing collagen for separation; this type of research allows for controlled medium and in-depth data analysis. Oxidative stress takes a toll on the domain, being beneficial as well as destructive. Reactive oxygen species (ROS) are molecules that influence the differentiation of osteoclasts, but over time their increasing presence can affect patients and aid the appearance of diseases such as osteoporosis. Oxidative stress can be limited by using antioxidants such as vitamin K and N‑acetyl cysteine (NAC). Scaffolds and biocompatible coatings such as hydroxyapatite and bioactive glass are required to isolate the implant, protect the zone from the metallic, ionic exchange, and enhance the bone regeneration by mimicking the composition and structure of the body, thus enhancing cell proliferation. The materials can be further functionalized with growth factors that create a better response and higher chances of success for clinical use. This review highlights the vast majority of newly obtained information regarding bone tissue engineering, such as new co-culturing models, implant coatings, scaffolds, biomolecules, and the techniques utilized to obtain them.

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Section: Mesenchymal Stem Cells Influence In Btementioning

confidence: 99%

Bone Regeneration and Oxidative Stress: An Updated Overview

et al. 2022

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“…These features include but are not limited to, micro- and nano-scale surface topography and patterning (Miller et al, 2001 ; Schmalenberg and Uhrich, 2005 ; Li et al, 2014 ; Yiannakou et al, 2017 ; Angelaki et al, 2020 ), cell imprinted topography (Moosazadeh Moghaddam et al, 2019 ), scaffold geometry (grooves, filaments, wells, pillars etc. ; Schmalenberg and Uhrich, 2005 ; Chew et al, 2008 ; Mitchel and Hoffman-Kim, 2011 ; Mobasseri et al, 2014 ; Tonazzini et al, 2015 ; Chen et al, 2019 ), hydrophilicity (Mobasseri et al, 2014 ), and roughness (Simitzi et al, 2015 ; Babaliari et al, 2021 ; Huang et al, 2021 ) and porosity (Li et al, 2014 ). Moreover, topographical characteristics are not investigated in a qualitative manner exclusively.…”

Section: The Effect Of the Microenvironment On Schwann Cell Behaviourmentioning

confidence: 99%

“…Gupta and co-workers studied the effect of shear stress on SC proliferation and genetic expression profiles and were able to find that under constant laminar fluid flow SCs had increased proliferation rates but displayed downregulation of MAG and myelin basic protein (MBP; Gupta and Steward, 2003 ; Gupta et al, 2005 ). Babaliari et al ( 2021 ) have studied the combined effect of shear stress and topography on SC behaviour under dynamic culture conditions attained via continuous flow. Experiments using a precise flow-controlled microfluidic system which incorporated laser-microstructured microgrooved polyethylene terephthalate (PET) substrates revealed that, depending on the relation of the direction of the flow with respect to the topographical features (parallel or perpendicular), wall shear stress gradients act in a synergistic or antagonistic manner to topography in promoting a guided morphological cell response (Babaliari et al, 2021 ).…”

Section: The Effect Of the Microenvironment On Schwann Cell Behaviourmentioning

confidence: 99%

“…Babaliari et al ( 2021 ) have studied the combined effect of shear stress and topography on SC behaviour under dynamic culture conditions attained via continuous flow. Experiments using a precise flow-controlled microfluidic system which incorporated laser-microstructured microgrooved polyethylene terephthalate (PET) substrates revealed that, depending on the relation of the direction of the flow with respect to the topographical features (parallel or perpendicular), wall shear stress gradients act in a synergistic or antagonistic manner to topography in promoting a guided morphological cell response (Babaliari et al, 2021 ).…”

Section: The Effect Of the Microenvironment On Schwann Cell Behaviourmentioning

confidence: 99%

“…Gupta and co-workers studied the effect of shear stress on SC proliferation and genetic expression profiles and were able to find that under constant laminar fluid flow SCs had increased proliferation rates but displayed downregulation of MAG and myelin basic protein (MBP; Gupta and Steward, 2003;Gupta et al, 2005). Babaliari et al (2021) have studied the combined effect of Mechanobiology and the Schwann cell response. SCs are affected by the mechanical properties of the engineered microenvironment, the underlying topography, and shear stress forces, which can determine their adhesion profiles, their ability to migrate and/or elongate, as well as their capacity to differentiate.…”

Section: The Effect Of Shear Stressmentioning

confidence: 99%

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The role of mechanobiology on the Schwann cell response: A tissue engineering perspective

Manganas

Kavatzikidou

Kordas

et al. 2022

Front. Cell. Neurosci.

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Schwann cells (SCs), the glial cells of the peripheral nervous system (PNS), do not only form myelin sheaths thereby insulating the electrical signal propagated by the axons, but also play an essential role in the regeneration of injured axons. SCs are inextricably connected with their extracellular environment and the mechanical stimuli that are received determine their response during development, myelination and injuries. To this end, the mechanobiological response of SCs is being actively researched, as it can determine the suitability of fabricated scaffolds for tissue engineering and regenerative medicine applications. There is growing evidence that SCs are sensitive to changes in the mechanical properties of the surrounding environment (such as the type of material, its elasticity and stiffness), different topographical features provided by the environment, as well as shear stress. In this review, we explore how different mechanical stimuli affect SC behaviour and highlight the importance of exploring many different avenues when designing scaffolds for the repair of PNS injuries.

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Engineering free-standing electrospun PLLCL fibers on microfluidic platform for cell alignment

Yildirim-Semerci,

Arslan-Yildiz

2024

Microfluid Nanofluid

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Here, a PLLCL-on-chip platform was developed by direct electrospinning of poly (L-lactide-co-ε-caprolactone) (PLLCL) on polymethyl methacrylate (PMMA) microfluidic chips. Designed microchip provides the electrospinning of free-standing aligned PLLCL fibers which eliminates limitations of conventional electrospinning. Besides, aligned fiber structure favors cell alignment through contactless manipulation. Average fiber diameter, and fiber alignment was evaluated by SEM analyses, then, leakage profile of microchip was investigated. 3D cell culture studies were conducted using HeLa and NIH-3T3 cells, and nearly 85% cell viability was observed in PLLCL-on-chip for 15 days, while cell viability of 2D control started to decrease after 7 days based on Live dead and Alamar Blue analyses. These findings emphasize biocompatibility of PLLCL-on-chip platform for 3D cell culture and its ability to mimic extracellular matrix (ECM). Immunostaining results prove that PLLCL-on-chip platform favors the secretion of ECM proteins compared to control groups, and cytoskeletons of cells were in aligned orientation in PLLCL-on-chip, while they were in random orientation in control groups. Overall, these results demonstrate that the developed platform is suitable for the formation of various 3D cell culture models and a potential candidate for cell alignment studies. Graphical Abstract

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Combined effect of shear stress and laser-patterned topography on Schwann cell outgrowth: synergistic or antagonistic?

Abstract: Shear stress can act either synergistically or antagonistically with topographical cues in specific cell responses such as orientation and elongation.

Cited by 10 publications

References 57 publications

Bone Regeneration and Oxidative Stress: An Updated Overview

Bone Regeneration and Oxidative Stress: An Updated Overview

The role of mechanobiology on the Schwann cell response: A tissue engineering perspective

Engineering free-standing electrospun PLLCL fibers on microfluidic platform for cell alignment

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