Combined Effect of Flaxseed Oil and Vitamin E Supplementation During Bull Semen Cryopreservation on Sperm Characteristics

Yuan, Chongshan; Wang, Hongtao; Li, Xichun; Liu, Hongyu; Zhao, Jing; Lu, Wenfa; Wang, Jun

doi:10.1089/bio.2021.0059

Cited by 7 publications

(5 citation statements)

References 48 publications

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“…It enhanced the motility, progressive motility, morphology and viability in frozen thawed goat sperm (Yimer et al, 2014). Similarly, the addition of DHA in the feed also enhanced the sperm characteristics in fresh-semen characteristics of bulls (Yuan et al, 2021;Gholami et al, 2010), boars (Rooke et al, 2001;Castellano et al, 2010), and rams (Samadian et al, 2010). These all above findings supports the result of the present study.…”

Section: Discussionsupporting

confidence: 90%

“…It has also been reported that dietary DHA improved the quality of fresh semen and increased DHA concentration in the ovine sperm membrane. Supplementation of oils (source of DHA) in feed has been reported to improve the fresh semen quality of various species, such as boars (Castellano et al, 2010), bulls (Yuan et al, 2021), stallion (Brinsko et al, 2005), and human (González-Ravina et al, 2018). It has also been shown that the in-vitro adding of DHA in boar semen results in improvement of motility and membrane integrity of frozen-thawed boar spermatozoa (Kaka et al, 2017).…”

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confidence: 99%

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Enhancement of Frozen-Thawed Quality of Bull Semen after Enrichment of Docosahexaenoic Acid Extended in Tris- Extender

Kaka¹,

Wahid²,

Yimer³

et al. 2022

JAHP

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Section: Discussionsupporting

confidence: 90%

mentioning

confidence: 99%

Enhancement of Frozen-Thawed Quality of Bull Semen after Enrichment of Docosahexaenoic Acid Extended in Tris- Extender

Kaka¹,

Wahid²,

Yimer³

et al. 2022

JAHP

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“…Finally, the results showed that ROS production and lipid peroxidation levels decrease significantly in NE treatment with respect to control under oxidative stress conditions. This effect has been described previously in red deer [ 12 , 14 , 16 , 21 ], bulls [ 46 , 47 ], boars [ 48 , 49 ] and humans [ 50 ] but never over such a long period of time. Through sperm storage, spermatozoa are subjected to several sources of oxidative stress, such as storage under low temperatures and the common oxidative metabolism in the mitochondrial [ 51 ].…”

Section: Discussionsupporting

confidence: 72%

Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams

Jurado-Campos,

Soria-Meneses,

Arenas-Moreira

et al. 2023

J Animal Sci Biotechnol

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Background Artificial insemination (AI) is a routine breeding technology in animal reproduction. Nevertheless, the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in AI. In this sense, nanotechnology is an interesting tool to improve sperm protection due to the development of nanomaterials for AI, which could be used as delivery vehicles. In this work, we explored the feasibility of vitamin E nanoemulsion (NE) for improving sperm quality during transport. Results With the aim of evaluating this proposal, ejaculates of 7 mature rams of Manchega breed were collected by artificial vagina and extended to 60 × 106 spz/mL in Andromed®. Samples containing control and NE (12 mmol/L) with and without exogenous oxidative stress (100 µmol/L Fe2+/ascorbate) were stored at 22 and 15 ºC and motility (CASA), viability (YO-PRO/PI), acrosomal integrity (PNA-FITC/PI), mitochondrial membrane potential (Mitotracker Deep Red 633), lipoperoxidation (C11 BODIPY 581/591), intracellular reactive oxygen species (ROS) production and DNA status (SCSA®) monitored during 96 h. Our results show that NE could be used to maintain ram spermatozoa during transport at 15 and 22 ºC for up to 96 h, with no appreciable loss of kinematic and physiological characteristics of freshly collected samples. Conclusions The storage of ram spermatozoa in liquid form for 2–5 d with vitamin E nanoemulsions may lead more flexibility to breeders in AI programs. In view of the potential and high versatility of these nanodevices, further studies are being carried out to assess the proposed sperm preservation medium on fertility after artificial insemination. Graphical Abstract

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“…Third, the protective effect of vitamin E against oxidative stress damage on spermatozoa has been reported previously in several species such as red deer [ 14 , 17 , 21 , 22 , 29 ], bulls [ 48 , 49 ], boars [ 50 , 51 ], or humans [ 52 ]. Overproduction of ROS during sperm handling is one of the major factors responsible for weakening the spermatozoa quality.…”

Section: Discussionmentioning

confidence: 89%

Vitamin E Lipid-Based Nanodevices as a Tool for Ovine Sperm Protection against Oxidative Stress: Impact on Sperm Motility

Jurado-Campos¹,

Soria-Meneses²,

Arenas-Moreira

et al. 2022

Antioxidants

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The advent of nanotechnology in the field of animal reproduction has led to the development of safer and more efficient therapies. The use of nanotechnology allows us to avoid the detrimental effects of certain traditional antioxidants, such as Vitamin E. Its hydrophobic nature makes mandatory the use of organic solvents, which are toxic to sperm cells. This study aims to evaluate the efficiency of vitamin E nanoemulsions (NE) on ram (Ovis aries) spermatozoa. For this purpose, the effect of three NE concentrations (6, 12, and 24 mM) were assessed on sperm of 10 mature rams of the Manchega breed. Sperm samples were collected by artificial vagina, pooled, and diluted in Bovine Gamete Medium. The samples were stored at 37 ºC and assessed at 0, 4, 8, and 24 h under oxidative stress conditions (100 µM Fe2+/ascorbate). Motility (CASA), viability (YO-PRO/IP), acrosomal integrity (PNA-FITC/IP), mitochondrial membrane potential (Mitotracker Deep Red 633), lipoperoxidation (C11 BODIPY 581/591), intracellular reactive oxygen species (ROS) production and DNA status (SCSA®®) were assessed. A linear mixed-effects models were used to analyze the effects of time, NE, and oxidant (fixed factors) on sperm parameters, and a random effect on the male was also included in the model with Tukey’s post hoc test. Protection of ram spermatozoa with NE resulted in a more vigorous motility under oxidative stress conditions with respect Control and Free vitamin E, while preventing the deleterious effects of oxidative stress coming from the production of free radicals and lipid peroxidation. These results ascertain the high relevance of the use of delivery systems for sperm physiology preservation in the context of assisted reproduction techniques.

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Combined Effect of Flaxseed Oil and Vitamin E Supplementation During Bull Semen Cryopreservation on Sperm Characteristics

Cited by 7 publications

References 48 publications

Enhancement of Frozen-Thawed Quality of Bull Semen after Enrichment of Docosahexaenoic Acid Extended in Tris- Extender

Enhancement of Frozen-Thawed Quality of Bull Semen after Enrichment of Docosahexaenoic Acid Extended in Tris- Extender

Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams

Vitamin E Lipid-Based Nanodevices as a Tool for Ovine Sperm Protection against Oxidative Stress: Impact on Sperm Motility

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