Combined effect of alpha particles and cigarette smoke on human lung epithelial cells <i>in vitro</i>

Lee, Ui-Seob; Kim, Eun‐Hee

doi:10.1080/09553002.2019.1625491

Cited by 5 publications

(4 citation statements)

References 44 publications

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“…Samples were handled carefully to avoid cell loss during the entire process. The cyto-centrifugation process took 1 h. The detailed subsequent experimental process was described in Lee et al 34 . The attached cells were washed with PBST, the Dulbecco’s phosphate buffered saline (DPBS) (Catalog No.…”

Section: Methodsmentioning

confidence: 99%

Characterization of γ-H2AX foci formation under alpha particle and X-ray exposures for dose estimation

Lee

Kim

2022

Sci Rep

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DNA double-strand break (DSB) induction is one of the phenotypes of cellular damage from radiation exposure and is commonly quantified by γ-H2AX assay with the number of excess fluorescent foci per cell as the main component. However, the number of foci alone may not fully characterize the state of DNA damage following exposures to different radiation qualities. This study investigated the feasibility of utilizing the focus size distribution and dephosphorylation rate of γ-H2AX to identify the type of causative radiation and dose. Human lung epithelial cells and mouse vascular endothelial cells were used to observe the expression changes of γ-H2AX foci due to alpha particle and X-ray exposures. Results showed that the average number of excess foci per cell linearly increased with the dose. The focus size distribution showed a consistent pattern depending on the causative radiation type. Three criteria for the identification of causative radiation type were derived from experimental focus size distributions and were validated in blind testing with correct identification of 27 out of 32 samples. The dose could be estimated based on the proportionality constant specific to the identified radiation type with a difference of less than 15% from the actual value. The different dephosphorylation rates of γ-H2AX produced from alpha particle and X-ray exposures were effectively utilized to determine the individual dose contributions of alpha particles and X-rays under mixed beam exposure. Individual doses were estimated to have differences of less than ~ 12% from actual values.

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Section: Methodsmentioning

confidence: 99%

Characterization of γ-H2AX foci formation under alpha particle and X-ray exposures for dose estimation

Lee

Kim

2022

Sci Rep

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“…Approximately one third of the studies listed in Tables 1 and 3 use cells of lung origin, of which a large fraction are the well-known Chinese hamster lung fibroblast cell line V-79 together with lung fibroblasts from rat origins [64,92,102,105,108]. However, some studies worked with BEAS-2B or other lung epithelial cell lines as well [67,68,84,104,116].…”

Section: Discussionmentioning

confidence: 99%

“…In a later study, Lee and Kim investigated the toxicity and effects of alpha-particle exposure on human lung epithelial cells (BEAS-2B, Null-1) and mouse vascular endothelial cells (SVEC4-10EHR1) by performing γ-H2AX staining and a clonogenic survival assay [116]. All three cell lines showed a dose-dependent linear increase in γ-H2AX foci per cell, with SVEC4-10EHR1 being most sensitive to alpha-particles.…”

Section: Americium-241 ( 241 Am)mentioning

confidence: 99%

Experimental Setups for In Vitro Studies on Radon Exposure in Mammalian Cells—A Critical Overview

Maier

Bailey

Hinrichs

et al. 2023

IJERPH

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Naturally occurring radon and its short lived progeny are the second leading cause of lung cancer after smoking, and the main risk factor for non-smokers. The radon progeny, mainly polonium-218 (238Pu) and polonium-214 (214Pu), are responsible for the highest dose deposition in the bronchial epithelium via alpha-decay. These alpha-particles release a large amount of energy over a short penetration range, which results in severe and complex DNA damage. In order to unravel the underlying biological mechanisms which are triggered by this complex DNA damage and eventually give rise to carcinogenesis, in vitro radiobiology experiments on mammalian cells have been performed using radon exposure setups, or radon analogues, which mimic alpha-particle exposure. This review provides an overview of the different experimental setups, which have been developed and used over the past decades for in vitro radon experiments. In order to guarantee reliable results, the design and dosimetry of these setups require careful consideration, which will be emphasized in this work. Results of these in vitro experiments, particularly on bronchial epithelial cells, can provide valuable information on biomarkers, which can assist to identify exposures, as well as to study the effects of localized high dose depositions and the heterogeneous dose distribution of radon.

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“…Moreover, chronic obstruction of lung can also affect the bronchial morphometry (see Section 5.1 below). Interestingly, smoking-induced asthma with the chronic obstruction in the lungs could affect the deposition of radon progeny in the lungs to synergistically increase the radon-induced lung cancer risk [69,70].…”

Section: Background For Focus In Present Reviewmentioning

confidence: 99%

Multiple Stressor Effects of Radon and Phthalates in Children: Background Information and Future Research

Kwan

Nikezić

Roy

et al. 2020

IJERPH

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The present paper reviews available background information for studying multiple stressor effects of radon (222Rn) and phthalates in children and provides insights on future directions. In realistic situations, living organisms are collectively subjected to many environmental stressors, with the resultant effects being referred to as multiple stressor effects. Radon is a naturally occurring radioactive gas that can lead to lung cancers. On the other hand, phthalates are semi-volatile organic compounds widely applied as plasticizers to provide flexibility to plastic in consumer products. Links of phthalates to various health effects have been reported, including allergy and asthma. In the present review, the focus on indoor contaminants was due to their higher concentrations and to the higher indoor occupancy factor, while the focus on the pediatric population was due to their inherent sensitivity and their spending more time close to the floor. Two main future directions in studying multiple stressor effects of radon and phthalates in children were proposed. The first one was on computational modeling and micro-dosimetric studies, and the second one was on biological studies. In particular, dose-response relationship and effect-specific models for combined exposures to radon and phthalates would be necessary. The ideas and methodology behind such proposed research work are also applicable to studies on multiple stressor effects of collective exposures to other significant airborne contaminants, and to population groups other than children.

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Combined effect of alpha particles and cigarette smoke on human lung epithelial cells in vitro

Cited by 5 publications

References 44 publications

Characterization of γ-H2AX foci formation under alpha particle and X-ray exposures for dose estimation

Characterization of γ-H2AX foci formation under alpha particle and X-ray exposures for dose estimation

Experimental Setups for In Vitro Studies on Radon Exposure in Mammalian Cells—A Critical Overview

Multiple Stressor Effects of Radon and Phthalates in Children: Background Information and Future Research

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