Combined detection of serum CTX-II and COMP concentrations in osteoarthritis model rabbits: an effective technique for early diagnosis and estimation of disease severity

Bai, Bin; Li, Yanqin

doi:10.1186/s13018-016-0483-x

Cited by 35 publications

(32 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The histological results in our study also have a corresponding experimental trend. This also shows that the articular cartilage has been obviously damaged from the second week in histology, and continued to aggravate with the passage of time, suggesting that CTX-II may also be used as a suitable biomarker for longitudinal study [28]. In addition, we used Mankin scoring system to explore the relationship between the concentration of CTX-II and the degree of articular cartilage degeneration.…”

Section: Discussionmentioning

confidence: 94%

Potential value of combining CTX-II with C2C in evaluating and diagnosing early rat osteoarthritis

Shen¹,

Zhang²,

Ma³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background This article aims to study the changes of CTX-II and C2C biomarker concentrations during the pathogenesis of osteoarthritis with SD rats as the research object. Through the longitudinal and joint detection of the content of CTX-II and C2C markers in the serum of anterior cruciate ligament resection (ACLT) rats, the evaluation and early diagnosis of OA disease can be realized, and the theoretical basis for the rapid diagnosis technology of OA can be provided. Methods SD rats were randomly divided into two groups: Sham operation group and OA model group. The OA model was established by anterior cruciate ligament excision (ACLT) for 2, 4, 6, 8, and 10 weeks, respectively. The cartilage gross score and Mankin score were used to evaluate the model. The tibia of the right hind limb was sampled for pathological histology and HE staining. The collected blood samples are used to detect changes in the concentration of CTX-II and C2C in serum. The receiver operating characteristic curve (ROC) was used to compare the area under the curve (AUC) of CTX-II, C2C and combined biomarkers. Results In the model group, the content of CTX-II and C2C in SD rat serum increased significantly, and the content of CTX-II and C2C increased with the prolonged modeling time, which was time dependent; The levels of CTX-II and C2C in the serum of rats in the sham operation group did not change significantly; both macroscopic observations and Mankin scores increased significantly. The AUC of the combined biomarker is higher than that of CTX-II and C2C. Finally, CTX-II and C2C levels were positively correlated with Mankin score. Conclusion Early combined detection of serum CTX-II and C2C concentrations has potential value for the evaluation and diagnosis of osteoarthritis.

show abstract

Section: Discussionmentioning

confidence: 94%

Potential value of combining CTX-II with C2C in evaluating and diagnosing early rat osteoarthritis

Shen¹,

Zhang²,

Ma³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…COMP serum concentrations were negatively correlated with disease duration, and positively associated with age, physical and sports activities. 96,97 Bai et al 98 induced OA in rabbits by anterior crucial ligament transsection and monitored the evolution of s-CTX-II and COMP at baseline and weeks 2, 4, 6, 8, 10, and 12 after the intervention. The CTX-II and COMP concentrations increased gradually to week 12, in parallel with the disease severity index Mankin score.…”

Section: Collagen Type II Degradation Products and Comp As Biomarkersmentioning

confidence: 99%

“…Histological findings confirmed a slow, but progressive cartilage deterioration. 98,99 In such a design, the CTX-II/COMP combination may be a marker for early diagnosis, evolution, and disease severity. In the study of Riegger et al, 100 conducted on 754 subjects who underwent total joint replacement, serum COMP levels positively correlated with markers of renal (cystatin C, creatinine, estimated glomerular filtration rate-eGFR) and cardiac (N-terminal propeptide of brain natriuretic peptide) function.…”

Section: Collagen Type II Degradation Products and Comp As Biomarkersmentioning

confidence: 99%

<p>Soluble Biomarkers of Osteoporosis and Osteoarthritis, from Pathway Mapping to Clinical Trials: An Update</p>

Nagy

Nagy-Finna

Popoviciu

et al. 2020

CIA

View full text Add to dashboard Cite

Serum biomarkers of osteoarticular diseases have been in the limelight of current clinical research trends. Laboratory validation of defined and candidate biomarkers for both osteoarthritis and osteoporosis is of key importance for future decisional algorithms in the diagnosis, monitoring, and prognosis of these diseases. The current guidelines recommend the use of collagen degradation remnants, eg, CTX-I and CTX-II, in the complementary diagnosis of both osteoporosis and osteoarthritis. Besides the collagen degradation markers, enzymes that regulate bone and articular metabolism are useful in the clinical evaluation of osteoarticular pathologies. Along these, several other recommended and new nominee molecules have been recently studied. Wnts and Wnt-related molecules have a cardinal role in the bone-joint homeostasis, making them a promising target not only for pharmaceutical modulation, but also to be considered as soluble biomarkers. Sclerostin and dickkopf, two inhibitor molecules of the Wnt/β-catenin signaling, might have a dual role in the assessment of the clinical manifestations of the osteoarticular unit. In osteoarthritis, besides fragments of collagen type II many pathway-related molecules have been studied and proposed for biomarker validation. The most serious limitation is that a significant proportion of studies lack statistical power due to the reduced number of cases enrolled. Serum biomarkers of bone and joint turnover markers represent an encouraging possibility for the diagnosis and prognosis of osteoarticular diseases, although further studies and laboratory validations should be carried out as to solely rely on them.

show abstract

“…Type II collagen peptide was obtained through collagen degradation of type II collagenase enzyme. C-telopeptides of type II collagen (CTX-II) are degradation products of joint tissues, particularly after the destruction of articular cartilage and cartilage extracellular matrix [12]. Therefore, CTX-II is considered to be a potential biomarker for cartilage destruction and to predict OA progression.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, CTX-II is considered to be a potential biomarker for cartilage destruction and to predict OA progression. [12]. A recent study showed that the development of CTX-II was closely related with knee OA [13].…”

Section: Introductionmentioning

confidence: 99%

Effects of Artesunate on the Expressions of Insulin-Like Growth Factor-1, Osteopontin and C-Telopeptides of Type II Collagen in a Rat Model of Osteoarthritis

Bai¹,

Guo²,

Tang³

et al. 2017

Pharmacology

View full text Add to dashboard Cite

Objective: The study aims to explore the effects of artesunate on insulin-like growth factor-1 (IGF-1), Osteopontin (OPN), and C-telopeptides of type II collagen (CTX-II) in serum, synovial fluid (SF), and cartilage tissues of rats with osteoarthritis (OA). Methods: OA models were established. Normal model, artesunate, and Viatril-S groups (20 rats respectively) were set. Enzyme-linked immunosorbent assay, IHC staining, and quantitative real-time polymerase chain reaction were conducted to calculate IGF-1, OPN, and CTX-II levels in serum, SF, and cartilage tissues of rats. The pathological changes in cartilage tissues were evaluated with Mankin score and Hematoxylin-Eosin staining. Results: Compared with the normal group, the model group showed increased IGF-1 level; decreased OPN, CTX-II levels in the serum and SF; and contrary results were seen in the cartilage tissues. A gradual ascending IGF-1 level and descending OPN and CTX-II levels existed in the serum and SF in the artesunate and Viatril-S groups after 2 weeks. The model group showed the most obvious pathological changes and highest Mankin score compared with the other groups. Higher IGF-1 level and lower OPN, CTX-II levels were exhibited in the cartilage tissue in the artesunate and Viatril-S groups but not in the model group. Conclusion: Artesunate and Viatril-S inhibit OA development by elevating IGF-1 level and reducing OPN and CTX-II levels.

show abstract

Combined detection of serum CTX-II and COMP concentrations in osteoarthritis model rabbits: an effective technique for early diagnosis and estimation of disease severity

Cited by 35 publications

References 27 publications

Potential value of combining CTX-II with C2C in evaluating and diagnosing early rat osteoarthritis

Potential value of combining CTX-II with C2C in evaluating and diagnosing early rat osteoarthritis

<p>Soluble Biomarkers of Osteoporosis and Osteoarthritis, from Pathway Mapping to Clinical Trials: An Update</p>

Effects of Artesunate on the Expressions of Insulin-Like Growth Factor-1, Osteopontin and C-Telopeptides of Type II Collagen in a Rat Model of Osteoarthritis

Contact Info

Product

Resources

About