Combined delivery of the adiponectin gene and rosiglitazone using cationic lipid emulsions

Davaa, Enkhzaya; Kang, Bong-Seok; Han, Joo‐Hui; Lee, Sang-Eun; Ng, Choon Lian; Myung, Chang‐Seon; Park, Jeong Sook

doi:10.1016/j.ijpharm.2015.02.015

Cited by 8 publications

(4 citation statements)

References 21 publications

(27 reference statements)

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“…oil and water) can be achieved through emulsification methods, which are broadly classified as either high- or low-energy processes 27–30 . Nanoscale emulsions with sizes on the order of 100 nm (so-called nanoemulsions) provide an efficient and facile approach to encapsulating both polar and non-polar functional biomolecules for co-delivery of dissimilar therapeutics 31–33 . Multiple strategies have been employed to obtain thermogelling emulsion-based organohydrogels which are mostly based on entrapping the dispersed phase via thermally gelling the continuous phase 34–37 .…”

Section: Introductionmentioning

confidence: 99%

Thermoresponsive nanoemulsion-based gel synthesized through a low-energy process

et al. 2019

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Thermoresponsive nanoemulsions find utility in applications ranging from food to pharmaceuticals to consumer products. Prior systems have found limited translation to applications due to cytotoxicity of the compositions and/or difficulties in scaling-up the process. Here, we report a route to thermally gel an oil-in-water nanoemulsion using a small amount of FDA-approved amphiphilic triblock Pluronic copolymers which act as gelling agents. At ambient temperature the suspension displays liquid-like behavior, and quickly becomes an elastic gel at elevated temperatures. We propose a gelation mechanism triggered by synergistic action of thermally-induced adsorption of Pluronic copolymers onto the droplet interface and an increased micelle concentration in the aqueous solution. We demonstrate that the system’s properties can be tuned via many factors and report their rheological properties. The nanoemulsions are prepared using a low-energy process which offers an efficient route to scale-up. The nanoemulsion formulations are well-suited for use in cosmetics and pharmaceutical applications.

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Section: Introductionmentioning

confidence: 99%

Thermoresponsive nanoemulsion-based gel synthesized through a low-energy process

et al. 2019

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“…A promising approach for both studying and treating metabolic syndrome involves the use of a class of drugs called thiazolidinediones [14–17], previously used to treat diabetes. Rosiglitazone (RGZ), the prototypical drug in this class, activates the nuclear peroxisome proliferator-activated receptor γ (PPARγ), a type II nuclear receptor, which in turn alters the expression of numerous genes [18], ultimately resulting in reduced blood concentrations of glucose, fatty acids, and insulin [19].…”

Section: Introductionmentioning

confidence: 99%

Regulatory landscape of AGE-RAGE-oxidative stress axis and its modulation by PPARγ activation in high fructose diet-induced metabolic syndrome

Cannizzaro

Rossoni

Savi³

et al. 2017

Nutr Metab (Lond)

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BackgroundThe AGE-RAGE-oxidative stress (AROS) axis is involved in the onset and progression of metabolic syndrome induced by a high-fructose diet (HFD). PPARγ activation is known to modulate metabolic syndrome; however a systems-level investigation looking at the protective effects of PPARγ activation as related to the AROS axis has not been performed.The aim of this work is to simultaneously characterize multiple molecular parameters within the AROS axis, using samples taken from different body fluids and tissues of a rat model of HFD-induced metabolic syndrome, in the presence or absence of a PPARγ agonist, Rosiglitazone (RGZ).MethodsRats were fed with 60% HFD for the first half of the treatment duration (21 days) then continued with either HFD alone or HFD plus RGZ for the second half.ResultsRats receiving HFD alone showed metabolic syndrome manifestations including hypertension, dyslipidemia, increased glucose levels and insulin resistance, as well as abnormal kidney and inflammatory parameters. Systolic blood pressure, plasma triglyceride and glucose levels, plasma creatinine, and albuminuria were significantly improved in the presence of RGZ. The following molecular parameters of the AROS axis were significantly upregulated in our rat model: carboxymethyl lysine (CML) in urine and liver; carboxyethyl lysine (CEL) in urine; advanced glycation end products (AGEs) in plasma; receptor for advanced glycation end products (RAGE) in liver and kidney; advanced oxidation protein products (AOPP) in plasma; and 4-hydroxynonenal (HNE) in plasma, liver, and kidney. Conversely, with RGZ administration, the upregulation of AOPP and AGEs in plasma, CML and CEL in urine, RAGE in liver as well as HNE in plasma and liver was significantly counteracted/prevented.ConclusionsOur data demonstrate (i) the systems-level regulatory landscape of HFD-induced metabolic syndrome involving multiple molecular parameters, including HNE, AGEs and their receptor RAGE, and (ii) attenuation of metabolic syndrome by PPARγ modulation.Electronic supplementary materialThe online version of this article (doi:10.1186/s12986-016-0149-z) contains supplementary material, which is available to authorized users.

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“…In considering these obstacles, non-viral gene carriers such as cationic polymers and lipids have been constructed based on their electrostatic interactions with DNA for gene delivery [21]. Cationic lipids, such as Lipofectamine [22,23] and 1,2-dioleoyl-3-trimethylammonium [24,25], are commonly used nonviral gene carriers owing to their high in vitro transfection efficiency. Furthermore, solid lipid nanoparticles can silence hepatitis C virus replication through delivering a shRNA targeted to internal ribosome entry site [26].…”

Section: Introductionmentioning

confidence: 99%

Neural differentiation of fibroblasts induced by intracellular co-delivery of Ascl1, Brn2 and FoxA1 via a non-viral vector of cationic polysaccharide

Chen

Deng

et al. 2017

Biomed. Mater.

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Direct reprogramming of other somatic cells into neurons is an alternative strategy for the recovery of an injured nervous system. In this work, we developed a new non-viral gene carrier based on Porphyra yezoensis polysaccharide (PYP). After modification with ethylenediamine, the cationized PYP (Ed-PYP) was combined with plasmids encoding Ascl1, Brn2 and FoxA1 to form spherical nanoscale particles (Ed-PYP-pABF nanoparticles). Cytotoxicity assays proved that Ed-PYP-pABF nanoparticles had a better safety profile than Lipofectamine 2000 and polyetherimide. Characterization tests illustrated that the Ed-PYP-pABF nanoparticles at an Ed-PYP:pABF weight ratio of 40:1 is a potential candidate for gene delivery, which was further supported by Western blot and plasmid encoding enhanced green fluorescence protein transfection. Based on this transfection strategy, we co-delivered pABF to 3T6 cells using Ed-PYP. ELISA indicated that the levels of brain-derived neurotrophic factor, nerve growth factors and sonic hedgehog reached a maximum at 14 days after the last transfection. Immunofluorescence and Western blot further exhibited positive expression of neurofilament 200, Nestin, glial fibrillary acidic protein, growth associated protein-43, β-3tubulin, and microtubule associated protein 2, proving the successful conversion of 3T6 cells into neurons. Taken together, these results illustrated that a natural polysaccharide-based gene co-delivery system is a promising strategy for neural reprogramming.

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Combined delivery of the adiponectin gene and rosiglitazone using cationic lipid emulsions

Cited by 8 publications

References 21 publications

Thermoresponsive nanoemulsion-based gel synthesized through a low-energy process

Thermoresponsive nanoemulsion-based gel synthesized through a low-energy process

Regulatory landscape of AGE-RAGE-oxidative stress axis and its modulation by PPARγ activation in high fructose diet-induced metabolic syndrome

Neural differentiation of fibroblasts induced by intracellular co-delivery of Ascl1, Brn2 and FoxA1 via a non-viral vector of cationic polysaccharide

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