In this study, chemical components of the dissolved organic matter (DOM) in effluents from yeast wastewater treatment plant were classified, the main compounds and their structures accounting for color and DOM were determined. The bases-insoluble matter (BIM, i.e. DOM!) were separated from the effluents, the reminders were fractionated through XAD-8 resin and saturated exchange resins into five groups: hydrophobic bases (HOB, i.e. DOM2), hydrophobic acids (HOA,i.e. DOM3), hydrophilic matter (HIM,i.e.DOM4), DOM5 and hydrophilic nertrals (HIN,i.e. DOM6). All the fractions were characterized by total organic carbon (TOC) analyzer and color determination. The results indicate that HOA is the most abundant fraction in the effluents, contributing to 31.2% of the DOM and nearly 90% of chromaticity. HIB and HIA are the second dominant fractions, accounting for 26.3% and 26.1 % of DOM, respectively. The rest of DOM fractions consists of 6.7% HOB, 5.9% BIM, 3.2% HIN. Further characterization of HOA by Solid phase extraction -Gas chromatograph-Mass spectrograph (SPE-GC/MS) demonstrates that the main compounds are mainly long-chain fatty acids and esters, such as tert-butyldimethylsilyl ester, dodecanoic acid, decanoic acid, n-tridecanoic acid trimethylsilyl ester and etradecanoic acid.