Combined biological and chemical assessment of estrogenic activities in wastewater treatment plant effluents

Aerni, Hans‐Rudolf; Kobler, Bernd; Rutishauser, Barbara V.; Wettstein, Felix E.; Fischer, Ren�; Giger, Walter; Hungerb�hler, Andreas; Marazuela, M.D.; Peter, Armin; Sch�nenberger, Ren�; V�geli, A. Christiane; Suter, Marc J.‐F.; Eggen, Rik I.L.

doi:10.1007/s00216-004-2555-8

Cited by 50 publications

(66 citation statements)

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“…These mechanisms were supported by many field studies involving wastewater treatment Sumpter 2001, Baronti et al 2000), in which good removal of E2 was obtained whereas E1 removal was not satisfactory. Since E1 still has relatively high estrogenic potency, its removal from wastewater is important in order to achieve an overall reduction in total estrogenic activity (Aerni et al 2004). The two strains, especially strain E2Y4, exhibited high E2-and E1-degrading capacities and thus hold great promise for conditions requiring the complete mineralization of estrogen.…”

Section: Discussionmentioning

confidence: 99%

“…The five strains decreased the estrogenic activities in the culture solution to 1/15 * 1/10 on day 1 and to 1/36 * 1/18 on day 15, as expressed as a fraction of the initial activity. Since the estrogenic activity of E1 is about 38-50% of that of E2, according to YES assays (Johnson and Sumpter 2001;Aerni et al 2004), the transformation by the isolates of E2 to E1 has the potential to reduce the estrogen activity of the test solution. Furthermore, the decrease in estrogenic activities obtained with strains E2Y4 and E2Y1 was most likely due to their ability to degrade both E2 and E1.…”

Section: E1 Degradation By the Isolated Strainsmentioning

confidence: 99%

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Isolation and characteristics of 17β-estradiol-degrading Bacillus spp. strains from activated sludge

2010

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The natural estrogen 17beta-estradiol (E2) is a major endocrine disruptor, with adverse effects on wildlife and humans. The aim of this study was to isolate microorganisms able to effectively remove E2 from wastewater. Accordingly, five E2-degrading strains of bacteria were isolated from activated sludge collected from a wastewater treatment plant. Based on their 16S RNA gene sequences, these five strains belonged to the genus Bacillus. All five isolates were capable of converting E2 to estrone (E1), greatly reducing total estrogenic activities in wastewater during E2 biodegradation. However, only two strains (strain E2Y1 and E2Y4) were able to further transform E1, whereas it accumulated in the culture medium of the other isolates. Among all isolates, strain E2Y4, with 100% of the 1,400 bp 16S RNA gene matched that of B. subtilis CICC10075, exhibited the highest E2 and E1 degradation capacities, degrading 1 mg E2/l completely within 4 days and further transforming 40% of the metabolite E1. Furthermore, the E2 degradation rates of strain E2Y4 increased with increasing initial concentrations of the steroid, with a high degradation capacity maintained even at initial concentrations up to 50 mg/l. These results demonstrate the potential significance of strain E2Y4 in biological remediation applications.

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Section: Discussionmentioning

confidence: 99%

Section: E1 Degradation By the Isolated Strainsmentioning

confidence: 99%

Isolation and characteristics of 17β-estradiol-degrading Bacillus spp. strains from activated sludge

2010

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“…As mentioned before, the LOD of the present YES bioassay was 8.7 E-03 ng EEQs/ ml. Previous studies have reported a wide range of LOD (0.01E-03 to 5.0E-02 ng E2/ml) for the YES bioassays (Fawell et al 2001;Aerni et al 2004;Lorenzen et al 2004). Our LOD is very close to the low range of the reported LOD values.…”

Section: Effects Of Yes Bioassay and Extraction Efficiency On Estrogementioning

confidence: 98%

Factors Affecting Distribution of Estrogenicity in the Influents, Effluents, and Biosolids of Canadian Wastewater Treatment Plants

Shieh

Louie

Law

2015

Arch Environ Contam Toxicol

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Canadian wastewater treatment plants (WWTPs) release significant amounts of estrogenic chemicals to nearby surface waters. Environmental estrogens have been implicated as the causative agents of many developmental and reproductive problems in animals, including fish. The goals of this study were to assess the estrogenic activity in the influents, effluents, and biosolids of thirteen Canadian WWTPs using the yeast estrogen screen (YES) bioassay and to investigate whether factors, such as wastewater treatment method, sample storage, extraction efficiency, population, and summer/winter temperature had any effects on the distribution of estrogenicity in the WWTPs. Results of the study showed that estrogenicity from the influent to the effluent decreased in seven WWTPs, increased in two WWTPs, and did not change in four WWTPs during the winter. Estrogenic concentrations generally decreased in the order of biosolids > influents > effluents and ranged from 1.57 to 24.6, 1.25E-02 to 3.84E-01, and 9.46E-03 to 3.90E-01 ng estradiol equivalents/g or ml, respectively. The estrogenicity in the final effluents, but not those in the influents and biosolids, was significantly higher in the summer than the winter. Among the WWTP treatment methods, advanced, biological nutrient removal appeared to be the most effective method to remove estrogenic chemicals from wastewaters in Canada. Our studies help to identify factors or mechanisms that affect the distribution of estrogenicity in WWTPs, providing a better understanding on the discharges of estrogenic chemicals from WWTPs.

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“…In parallel, the importance of detecting micropollutants was emphasized through the development of biotests (e.g., specialized to identify compounds with endocrine disrupting properties), which pointed out to the high biological activity of some class of micropollutants. For instance, the ubiquitous distribution in the environment of EDCs and their harmful potential was emphasized through the development of very sensitive biological tests based on immunological techniques such as ELISA or on endocrine functions such as yeast estrogen screen (YES) (Huang and Sedlak 2001;Aerni et al 2003;Bringolf and Summerfelt 2003;Matsunaga et al 2003) E-SCREEN assay (Soto et al 1995), EROD activity assay (Ma et al 2005) or combination of bioassays (Oh et al 2006). Part of these studies concluded that some of these compounds [e.g., alkylphenol ethoxylates, bisphenol A (BPA), estrone (E1), 17b-estradiol (E2), 17a-ethinylestradiol (EE2)] can have high (specific biological) estrogenic activity even at extremely low concentration (Purdom et al 1994;Jobling et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Factors affecting the removal of organic micropollutants from wastewater in conventional treatment plants (CTP) and membrane bioreactors (MBR)

Cirja

Ivashechkin

Schäffer

et al. 2007

Rev Environ Sci Biotechnol

272

153

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As a consequence of insufficient removal during treatment of wastewater released from industry and households, different classes of organic micropollutants are nowadays detected in surface and drinking water. Among these micropollutants, bioactive substances, e.g., endocrine disrupting compounds and pharmaceuticals, have been incriminated in negative effects on living organisms in aquatic biotope. Much research was done in the last years on the fate and removal of those compounds from wastewater. An important point it is to understand the role of applied treatment conditions (sludge retention time (SRT), biomass concentration, temperature, pH value, dominant class of micropollutants, etc.) for the efficiency of conventional treatment plants (CTP) and membrane bioreactors (MBR) concerning the removal of micropollutants such as pharmaceuticals, steroid- and xeno-estrogens. Nevertheless, the removal rates differ even from one compound to the other and are related to the ph ysico-chemical characteristics of the xenobiotics

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Combined biological and chemical assessment of estrogenic activities in wastewater treatment plant effluents

Cited by 50 publications

References 0 publications

Isolation and characteristics of 17β-estradiol-degrading Bacillus spp. strains from activated sludge

Isolation and characteristics of 17β-estradiol-degrading Bacillus spp. strains from activated sludge

Factors Affecting Distribution of Estrogenicity in the Influents, Effluents, and Biosolids of Canadian Wastewater Treatment Plants

Factors affecting the removal of organic micropollutants from wastewater in conventional treatment plants (CTP) and membrane bioreactors (MBR)

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