Combined biocompatible medium with molecularly imprinted polymers for determination of aflatoxins B1 in real sample

Abstract

A kind of molecularly imprinted polymers modified with biocompatible medium was prepared by suspension polymerization. The obtained hybrid materials were used as the adsorbents for the solid‐phase extraction of aflatoxins B1 in real samples. A structural analog of the target, 6‐methyl‐4‐phenylchroman‐2‐one was used as the pseudo‐template, owing to their lower toxicity and cheaper price compared with aflatoxins B1; and methacrylic acid and glycidyl methacrylate were used as the co‐monomers. Scanning electron microscopy and size distribution analysis were used to characterize the obtained polymers. The extraction parameters were optimized to achieve the desired extraction performance. The polymer solid‐phase extraction coupled with high‐performance liquid chromatography was successfully applied to determine aflatoxins B1 from soy sauce without the process of protein removal. Under the optimum extraction conditions, the detection results of aflatoxins B1 in lab‐made column in soy sauce samples was carried out, with a recovery rate of 96%. The established method presented a linear range from 10 to 1000 ng/mL with the coefficient of determination of 0.9994 and the limit of detection of 0.05 ng/mL. Likewise, the inherent selectivity of lab‐made column towards aflatoxins B1, Ochratoxin A, and Zearalenone was demonstrated.

Molecularly Imprinted Polymers in Biological Applications

“…Iridoid glycosides, spiramycin, 2,4-dichlorophenoxyacetic acid, diclofenac, alfatoxins [17][18][19][20][21] Suspension polymerization Malachite green, p-hydroxybenzoic acid, timolol, alfatoxins B1, Pb 2+ [22][23][24][25][26] Emulsion polymerization Quercetin, malachite green, Listeria monocytogenes, triazines, Cd 2+ [27][28][29][30][31] Seed polymerization Nicotinamide, bisphenol A, promazine derivates [32][33][34] Precipitation polymerization Triclosan and triclocarban, strychnine, caffeic acid, vitamin E, prednisolone [35][36][37][38][39] RAFT polymerization Benzimidazole, propranolol, polycyclic aromatic hydrocarbons [40][41][42] Atom transfer radical polymerization Ofloxacin, ␤2-agonists [43,44] Sol-gel Iprodione, methylxanthines [45,46] RAFT: Reversible addition fragmentation chain transfer.…”

Recent advances and applications of molecularly imprinted polymers in solid‐phase extraction for real sample analysis

“…Several traditional polymerization approaches, including suspension [100,101], mini emulsion [102], and multistep seed swelling polymerization [103], and the new Pickering emulsion polymerization [104] were also utilized for the synthesis of spherical MIPs. For example, a kind of biocompatible dummy MIP nanoparticles prepared by suspension polymerization was used for SPE and determination of aflatoxins B1 in soy sauce without the process of protein removal [100], and a type of MIP micron spheres obtained by miniemulsion polymerization for SPE and quantification of aflatoxin M 1 and B 1 in different foods and feeds [102].…”

Application of molecularly imprinted polymers for the separation and detection of aflatoxin

“…15 Reported dummy template molecules include 5,7-dimethoxycoumarin (DMC), 2 quercetin, 16 7-acetoxy-4-methylcoumarin, 17 and 6-phenyl-4-methyl-2-chromanone ( Figure 3). 18 DMC is a common template molecule for the preparation of AF MIPs.…”

“…Song et al 18 reported a hydrophilic MIP prepared by suspension polymerization. Glycidyl methacrylate (GMA) is employed to regulate the hydrophilicity of the polymer, and polyvinyl alcohol is used as a suspending agent ( Figure 5).…”