Combined bead-based multiplex detection of RNA and protein biomarkers: Implications for understanding the time course of skeletal muscle injury and repair

Gary, Melody A.; Tanner, E; Davis, Asheal A.; McFarlin, Brian K.

doi:10.1016/j.ymeth.2018.11.012

Cited by 7 publications

(6 citation statements)

References 34 publications

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“…The average 30-day training data for the two groups are found in Table 1 . The sample size for the present study was determined based on previous studies from our laboratory using a similar supplement and exercise model ( Gary et al, 2019 ; Tanner et al, 2020a , b ). These previous studies included between 10 and 20 subjects, which was similar to the enrollment of the present study.…”

Section: Methodsmentioning

confidence: 99%

Dietary Polyphenol and Methylsulfonylmethane Supplementation Improves Immune, DAMP Signaling, and Inflammatory Responses During Recovery From All-Out Running Efforts

et al. 2021

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Nutritional ingredients with defined mechanisms of action can be useful in the recovery of the body from the physical demands of a habitual training plan. The purpose of this study was to determine the effect of dietary supplementation with optimized curcumin, pomegranate ellagitannins, and MSM (R + MSM) on immune-associated mRNA during early recovery (i.e., up to 8 h post-exercise) following all-out running efforts (5-km, 10-km, and 21.1-km). Subjects (N = 14) were randomized to either a supplement (R + MSM) or a control group using an open label design. The study was completed over a period of 31-day prior to a scheduled half-marathon race. Venous blood samples were collected into PAXgene tubes at baseline, subsequent samples were collected at 2, 4, and 8 h after each running effort. A 574-plex mRNA Immunology Array (NanoString) was measured for each sample and ROSALIND® Advanced Analysis Software was used to examined changes in 31 annotated immune response pathways and specific mRNA changes. The greatest change in immune pathways occurred at 2 h (GSS > 3) followed by 4 h (GSS 2–3) and 8 h (GSS 1–2). R + MSM was associated with an increase in innate immunity (CAMP, LTF, TIRAP, CR1, IL1R1, CXCR1, PDCDILG2, and GNLY) and a blunted/smaller increase in damage-associated molecular pattern (DAMP) signaling/inflammation (TLR4, TLR5, S100A8, S100A9, and IFP35). We also found changes in immune-associated mRNA that have not been previously linked to exercise recovery (SOCS1, SOCS2, MME, CECAM6, MX1, IL-1R2, KLRD1, KLRK1, and LAMP3). Collectively these results demonstrate that supplementation with a combination of optimized curcumin, pomegranate ellagitannins, and methylsulfonylmethane resulted in changes that may improve biological recovery from all-out running efforts.

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Section: Methodsmentioning

confidence: 99%

Dietary Polyphenol and Methylsulfonylmethane Supplementation Improves Immune, DAMP Signaling, and Inflammatory Responses During Recovery From All-Out Running Efforts

et al. 2021

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“…A previous study identified the latent correlation between lncRNA-mRNAs and ET as novel biomarker as well as therapeutic strategy for vascular endothelial IRI. Furthermore, lncRNA THRIL, as a transcriptional regulator of TNF-α, was altered after a half marathon race and was speculated beyond of NF-kB signaling pathway (Gary et al, 2019).…”

Section: Lncrnas and Circrnas Respond To Cardiac-protection By Exercisementioning

confidence: 99%

Exercise Mediates Heart Protection via Non-coding RNAs

Zhang

Feng

et al. 2020

Front. Cell Dev. Biol.

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Cardiovascular diseases (CVDs) have become the central matter of death worldwide and have emerged as a notable concern in the healthcare field. There is accumulating evidence that regular exercise training can be as a reliable and widely favorable approach to prevent the heart from cardiovascular events. Non-coding RNAs (ncRNAs) could act as innovative biomarkers and auspicious therapeutic targets to reduce the incidence of CVDs. In this review, we summarized the regulatory effects of ncRNAs in the cardiac-protection provided by exercise to assess potential therapies for CVDs and disease prevention.

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“…One design variable that can be gleaned from rodent studies is that in order for curcumin to be successful, it appears to have the best efficacy when the quantity of soreness/inflammation is moderate, but not severe. Excessive soreness/ inflammation completely overwhelms the biological system, resulting in changes that are unlikely to be managed with oral curcumin supplementation or any other treatment [11,30]. Soreness is a difficult result to quantify and accurately assess due to individual differences in perception of pain and soreness [10].…”

Section: Previous Applications Of Curcumin For Muscle Recoverymentioning

confidence: 99%

“…Our response are similar to Jager [18] who reported that a higher dose of optimized curcumin (CurcuWIN ® ; 1000 mg/day) resulted in maintenance of muscle strength following downhill running, while a lower dose (250 mg/ day) or placebo resulted in loss of muscle strength. To test our laboratory findings in a field model, we conducted a half-marathon training study to determine if optimized curcumin (Longvida ® ; 500-1000 mg/day) in combination with another dietary polyphenol (pomegranate extract; Pomella ® ; 500-1000 mg/day) could manage training-associated changes in inflammation [12,35]. When compared to a placebo, we found that the treatment allowed for 11% more training mileage and 20% more caloric expenditure despite a similar number of training sessions in the 30 days prior to a half marathon race.…”

Section: Previous Applications Of Curcumin For Muscle Recoverymentioning

confidence: 99%

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Does Acute Improvement in Muscle Recovery with Curcumin Supplementation Translate to Long-Term Training?

McFarlin

Tanner

Gary

et al. 2019

J. of SCI. IN SPORT AND EXERCISE

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Dietary polyphenols, such as curcumin, green tea catechins, and pomegranate extract, may have the ability to enhance the effectiveness of long-term training programs by managing training-induced inflammation. Enhanced recovery may translate to increased capacity to train and perform more effectively. The dietary polyphenol curcumin has been reported to block the action of COX-2 and NF-kB signaling and would allow for increased capacity to train leading to heightened adaptations and the potential for ergogenic outcomes. These actions are very similar to the targeted actions of non-steroidal anti-inflammatory drugs (NSAIDs), but without the side-effects of NSAIDs. This review will compare and contrast the known effects of curcumin and identify common design elements between existing studies. Through this critical review of the existing literature it is our goal to establish a set of best practices that could be applied to an athletic population that is interested in using oral curcumin supplementation as a recovery agent.

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Combined bead-based multiplex detection of RNA and protein biomarkers: Implications for understanding the time course of skeletal muscle injury and repair

Cited by 7 publications

References 34 publications

Dietary Polyphenol and Methylsulfonylmethane Supplementation Improves Immune, DAMP Signaling, and Inflammatory Responses During Recovery From All-Out Running Efforts

Dietary Polyphenol and Methylsulfonylmethane Supplementation Improves Immune, DAMP Signaling, and Inflammatory Responses During Recovery From All-Out Running Efforts

Exercise Mediates Heart Protection via Non-coding RNAs

Does Acute Improvement in Muscle Recovery with Curcumin Supplementation Translate to Long-Term Training?

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