Combined autofluorescence and Raman spectroscopy method for skin tumor detection in visible and near infrared regions

Abstract

The combined application of Raman and autofluorescence spectroscopy in visible and near infrared regions for the analysis of malignant neoplasms of human skin was demonstrated. Ex vivo experiments were performed for 130 skin tissue samples: 28 malignant melanomas, 19 basal cell carcinomas, 15 benign tumors, 9 nevi and 59 normal tissues. Proposed method of Raman spectra analysis allows for malignant melanoma differentiating from other skin tissues with accuracy of 84% (sensitivity of 97%, specificity of 72%). Autofluorescence analysis in near infrared and visible regions helped us to increase the diagnostic accuracy by 5-10%. Registration of autofluorescence in near infrared region is realized in one optical unit with Raman spectroscopy. Thus, the proposed method of combined skin tissues study makes possible simultaneous large skin area study with autofluorescence spectra analysis and precise neoplasm type determination with Raman spectroscopy.

Акустооптический Гиперспектральный Модуль Для Гистологического Исследования Микрообъектов

“…Для решения этой задачи гиперспектральные системы могут работать как совместно с эндоскопами для исследования биологических тканей in vivo [4], так и в составе микроскопов для исследований in vivo [5,6] и in vitro [7]. Оба подхода могут дополнить существующие методы диагностики рака, прежде всего, морфологическое и иммуногистохимическое исследование, флуоресцентную спектроскопию [8], лазерную автофлуоресцентную поляриметрию [9], спектроскопию комбинационного рассеяния [10].…”