Combined approach of selective and accelerated cloning for microfluidic chip‐based system increases clone specific productivity
Caroline Desmurget,
Julie Frentzel,
Anastasiya Strembitska
et al.
Abstract:Improving current cell line development workflows can either focus on increasing the specific productivity of the cell lines or shortening timelines to reach the clinic as fast as possible. In this work, using the Beacon platform, we have combined two distinct protocols – early cloning with low‐viability pools, and IgG membrane staining‐, to concomitantly reach both objectives, and generate highly productive CHO clones in shorter timelines. Fast‐sorting approaches using low‐viability pools in combination with … Show more
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