Combined application of dazomet and Trichoderma asperellum as an efficient alternative to methyl bromide in controlling the soil-borne disease complex of bell pepper

Slusarski, C.; Pietr, S J

doi:10.1016/j.cropro.2009.03.016

Cited by 44 publications

(25 citation statements)

References 27 publications

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“…Biocontrol agents are becoming increasingly important in achieving these goals while simultaneously enhancing crop production. However, some problems such as relatively low efficiency and instability of biopesticides have limited their development so far (Kredics et al 2003; Slusarski and Pietr 2009). More biocontrol agents with high efficiency will be obtained with genetic improvement.…”

Section: Discussionmentioning

confidence: 99%

Transformation of the endochitinase gene Chi67-1 in Clonostachys rosea 67-1 increases its biocontrol activity against Sclerotinia sclerotiorum

et al. 2017

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Clonostachys rosea is a promising biocontrol fungus active against various plant fungal pathogens. In this study, the endochitinase-encoding gene Chi67-1, the expression of which is sharply upregulated in C. rosea 67-1 when induced by sclerotia, was transformed into the original isolate by protoplast transformation, and transformants were screened against Sclerotinia rot of soybean. The transformation efficiency was approximately 50 transformants per 1 × 107 protoplasts, and 68 stably heritable recombinants were assayed. The parasitic rates of 32.4% of the tested strains increased by more than 50% compared to 43.3% of the wild type strain in 16 h, and the Rc4-4 transformant showed a parasitic rate of 100% in 16 h. The control efficiencies of the selected efficient transformants to soybean Sclerotinia stem rot were evaluated in pots in the greenhouse, and the results revealed that Rc4-4 achieved the highest efficiency of 81.4%, which was 31.7% and 28.7% higher than the control achieved by the wide type and the pesticide carbendazim, respectively. Furthermore, the expression level of Chi67-1 was 107-fold higher in Rc4-4 than in the wild type, and accordingly, the chitinase activity of the recombinant increased by 140%. The results lay a foundation for the development of efficient genetically engineered strains of C. rosea.

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Section: Discussionmentioning

confidence: 99%

Transformation of the endochitinase gene Chi67-1 in Clonostachys rosea 67-1 increases its biocontrol activity against Sclerotinia sclerotiorum

et al. 2017

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“…Ten Trichoderma isolates were tested by D'Ercole et al (2000) and Trichoderma viride T46 and T117 resulted in the best protection with a reduction of the disease incidence of 30% in eggplant. Three strains reduced the disease with more than 80% in tomato, eggplant and pepper (Dutta, 1981; Narisawa et al, 2002; Ślusarski and Pietr, 2009). In the case of respectively Trichoderma asperellum B35 and Trichoderma harzianum T-35, the efficacy of control depended on several factors such as the field location of the experiments and the type of formulation (Ordentlich et al, 1990; Ślusarski and Pietr, 2009).…”

Section: Biological Control Of Verticillium Wiltmentioning

confidence: 99%

Desirable Traits of a Good Biocontrol Agent against Verticillium Wilt

et al. 2017

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The soil-borne fungus Verticillium causes serious vascular disease in a wide variety of annual crops and woody perennials. Verticillium wilt is notoriously difficult to control by conventional methods, so there is great potential for biocontrol to manage this disease. In this study we aimed to review the research about Verticillium biocontrol to get a better understanding of characteristics that are desirable in a biocontrol agent (BCA) against Verticillium wilt. We only considered studies in which the BCAs were tested on plants. Most biocontrol studies were focused on plants of the Solanaceae, Malvaceae, and Brassicaceae and within these families eggplant, cotton, and oilseed rape were the most studied crops. The list of bacterial BCAs with potential against Verticillium was dominated by endophytic Bacillus and Pseudomonas isolates, while non-pathogenic xylem-colonizing Verticillium and Fusarium isolates topped the fungal list. Predominant modes of action involved in biocontrol were inhibition of primary inoculum germination, plant growth promotion, competition and induced resistance. Many BCAs showed in vitro antibiosis and mycoparasitism but these traits were not correlated with activity in vivo and there is no evidence that they play a role in planta. Good BCAs were obtained from soils suppressive to Verticillium wilt, disease suppressive composts, and healthy plants in infested fields. Desirable characteristics in a BCA against Verticillium are the ability to (1) affect the survival or germination of microsclerotia, (2) colonize the xylem and/or cortex and compete with the pathogen for nutrients and/or space, (3) induce resistance responses in the plant and/or (4) promote plant growth. Potential BCAs should be screened in conditions that resemble the field situation to increase the chance of successful use in practice. Furthermore, issues such as large scale production, formulation, preservation conditions, shelf life, and application methods should be considered early in the process of selecting BCAs against Verticillium.

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“…Although soil fumigation with bromomethane is an effective control method, due to environmental concerns, it is scheduled to be banned worldwide in 2015 (Shen, 2011). A recent focus has been the development of efficacious and environmentally friendly methods using field trials at multiple scales (Bailey and Lazarovits, 2003;Slusarski and Pietr, 2009;Thuerig et al, 2009). Based on both effectiveness and environmental safety, one of the most promising control measures to replace bromomethane is the use of brassicaceous seed meal (BSM) as a soil amendment.…”

Section: Introductionmentioning

confidence: 99%

Impact of brassicaceous seed meals on the composition of the soil fungal community and the incidence of Fusarium wilt on chili pepper

Gentry

et al. 2015

Applied Soil Ecology

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Combined application of dazomet and Trichoderma asperellum as an efficient alternative to methyl bromide in controlling the soil-borne disease complex of bell pepper

Cited by 44 publications

References 27 publications

Transformation of the endochitinase gene Chi67-1 in Clonostachys rosea 67-1 increases its biocontrol activity against Sclerotinia sclerotiorum

Transformation of the endochitinase gene Chi67-1 in Clonostachys rosea 67-1 increases its biocontrol activity against Sclerotinia sclerotiorum

Desirable Traits of a Good Biocontrol Agent against Verticillium Wilt

Impact of brassicaceous seed meals on the composition of the soil fungal community and the incidence of Fusarium wilt on chili pepper

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